Western Blot

( www.abnova.com ) - Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein. More videos at Abnova www.abnova.com

[@XSirApocalypseX]

We learn those techniques (Microarray, Western Blot, Gel-electrophoresis, Southern and Northern Blot) in university theoretical and later practical and it's interesting to see it how it works.

[@NoneroneousX5]

Wow. Real science one step at a time all lined up for us to see and made to look so easy. You had to have worked very hard too. thank you

[@CR-uu1rr]

Excellent and very clear. Detailed step by step. Amazing

[@nicklinkzelda]

@XSirApocalypseX - that's how it works at my uni, too! We only briefly discussed Western blotting in a grad level course I took and now I'm actually doing Westerns in a lab! It is definitely much more different than what I imagined.

[@Lucuskane]

Best video I've watched on this site.

[@reinaldofranqui1]

i have a test about this tomorrow i did it in the lab but this was waaay more comprehensive thanx!!!

[@nicklinkzelda]

Karyotyping sounds amazing... though it is probably contamination prone considering the process. That's not something they even mentioned you could even do at my university. Thanks for sharing that bit of into.

[@Giaca89]

Exactly, you have to remember that you have many strips as the number of the samples you have added in wells for electrophoresys! You just have to remember which strips correspond to your samples ;)

[@lovy42]

why haven't they put the chemiluminescent reagent directly on the membrane..?

[@salesmilton]

Is this test used for a single patient or multiple patients? If multiple, what is the maximum number of patients that can be tested.

[@samsaras]

yes very superb professional handling skills. i'm gonna go into lab work.

[@mouaynicholas]

lol we're doing this for our showcase project at cart. it's not that its hard, but you just gotta be careful on what your adding. for us, we have to start from scratch. making the blotting buffer, sample buffer, primary antibodies, secondary antibodies....yeesh. this is such a long project and a bit hard to understand.

[@priboechaat]

This really helped me! Thank you very much! Great video!

[@millie7995]

Would higher or lower percentage gels favour transfer to a membrane

[@danielhdoro]

Seems easy in the video. Way different in a lab like mine.

[@khadidjabelgardi8624]

who can help me I need principle and the pros and cons of this procedure

[@sonalverma905]

supperbbbbb ..!!!! nice explanation ..thanks a lot!! :-)

[@andiaprido2721]

That's good.

[@XSirApocalypseX]

@nicklinkzelda That's really interesting, but what I want to do is to do a karyogramm, we've genetics, als human-genetics. Sorry for my bad english.

[@kennetthkogo]

realy good

[@ivanwolff]

very complicated.

[@leminhduc93]

i like that centrifuger :)

[@prudvipeddireddi]

too complicated