2D Gel Electrophoresis (1) Protein Extraction

( www.abnova.com ) - Pure protein is the preliminary requirement of performing successful 2D gel electrophoresis. Our scientist showed you how to extract your proteins of interest by using a protein extraction kit followed by isopropanol precipitation and acetone wash steps. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight). More videos at Abnova www.abnova.com

[@tanvirrahman869]

verbal description will make this video much better.

[@learnersseekers904]

will this method work for plant tissue cell

[@bxm006]

Will this method work for any sample? Will it for bacterial cell sample? Will it work for plant cells?