Why your plasmid runs weird on an agarose gel: DNA topology meets agarose gel electrophoresis

[@31-canaan44]

great video for learning about electrophoresis!! it's simple, fun, and yet full of knowledge.

[@thebumblingbiochemist]

Thanks so much - so glad you found it helpful!

[@sophiebaldwin5380]

this is super interesting! I’ve been learning something similar at university lately. Great video! :)

[@thebumblingbiochemist]

Thank you so much! And best of luck with school :)

[@arkanshsharma850]

Best explanation on youtube

[@arkanshsharma850]

nice work really appreciate your efforts thanks for the awesome explanation

[@thebumblingbiochemist]

Thank you so much!

[@dr.zainababdulameer4655]

Thank you that is great, is there a difference between gram-positive and negative plasmid?

[@thebumblingbiochemist]

The plasmids will have different elements (different forms of promoters, etc.) depending on their compatibility with the bacterial type. Practically, the biggest difference in the lab comes from needing to use harsher lysis methods to extract plasmids from gram-positive bacteria.