Yeast two hybrid system | Protein - protein interaction

The yeast two hybrid system is used to study,
protein protein interaction.

[@peachyuumii9320]

You have been saving my semesters the entire graduation period and i cannot be more thankful. I sincerely hope your channel grows and you have the much deserved sucess ❤

[@riyamazumdar3849]

So beautiful explained.All doubt cleared about yeast two hybrid system. Y2H system is used to study protein - protein interaction. Suppose we need to study about the interaction between Protein x and Protein y, then the 1st step is to ligate these two protein in two separate plasmid with Gal 4 AD and Gal 4 BD(Gal 4 AD and Gal 4BD are transcription factors which forms a heterodimer and bind in the promter and switch on the transcription and a reporter protein is formed which can either be a fluorescence gene or Lacz gene). 2nd step, Both the plasmids are transformed inside the yeast cell.(Note: Gal 4 AD & Gal 4 BD should be knockout from the yeast genome) Now, the yeast will form Gal4 AD & Gal 4 BD along with Protein x & Protein y in such a way that, only when the gal 4 AD & gal 4 BD will come closer to form a complex and bind in the promoter region when the two proteins Protein x & Protein y interact and form a complex. So, if the two proteins interect the reporter gene will give rise to reporter protein,so we will conclude that the Protein x & Potein y interact with each other.

[@lynnvanboxtel3258]

love this explanation. i missed a lesson and was stressed but it is so clear now. even better than the notes of a classmate.

[@kevintan1141]

Ur explanation is even better than my so called Professor! Well done!

[@mohammadfahmawie162]

thank you so much for this amazing explaination to this topic

[@quantumcry8051]

Explained very well!

[@Me_Kohinoor]

Thank you so much for saving my day to understand this topic ❣️✨

[@prernavinaik]

Thank you so much such an easy explanation 💕

[@catherine_chipa]

Wow thank you!! This is amazing

[@bismaseher7800]

Awesome explanation sir Thank you sooo much..

[@chitramayamongar984]

Hello admin you covered all topics such as agarose gel electrophoresis,plasmid isolation and enzyme kinetics, can you also make for transaminase and carbohydrate metabolism Biochemistry lab part please,it will be very useful. Further more I am your big fan because I was about to quit biomedical science degree because I didn’t understand biochemistry until I found your vidoe😅.Summarizing all in 2 min video is a big thing.I will be more grateful if you can make video on hormone,vitamin and nutrition,control and interrelation of metabolism,genetic engineering biochemistry lecture parts,it will be very grateful for the future students who will watch your video,😊I cannot do anything in return but I will prompt your video to have 1M subscribers.❤

[@c.wredacted2589]

Thank you so much it really helped me alot✨✨

[@esharajput1301]

Hy your explanation is soo easy to understand , can you please make a video on crispr cas9 as soon as possible 🙏

[@sevodar]

Nice video but Sir i think you switched between the AD and BD ( Binding) sites on the sequence

[@dereksniper]

good & simple for review

[@vineypreetakkal6402]

Thank you