Yeast-two-hybrid screen (Y2H)

[@shemaiahmoore5904]

Y2H is so much easier to comprehend now. Thank you so much for covering this topic!

[@LeonEllisZ]

This is the best Y2H video I've come across that actually makes sense!

[@matthewmangus6929]

This video is a national treasure.

[@marwaatef4898]

Thank you so much for your clear and unambiguous explanations.

[@femiadepoju1389]

This is one of the best videos on Y2H. Thank you for making this.

[@ajahan9105]

Brief but clear enough to understand!

[@themoodz9490]

Easy to understand explanation. Thanks Henrik's lab!

[@abeer8733]

Very well explained in a super simple way. Really liked it!

[@blountyhunter1966]

Great intro into Y2H, clear and concise! Thanks!

[@alpaslanaksoy533]

Thank you very much Henrik! You are doing a wonderful job with your videos.

[@henrikslab]

Thanks!

[@Unokatziba]

This is the best Y2H video on youtube - so easy now to understand, thanks a lot!

[@medielijah]

Clear, concise, helpful! Thanks!!

[@alexandertomlinson4517]

This was so helpful as I am doing a project right now. Thank you!!

[@hadileghaddar]

So clearly explained! Thank you!!

[@grayceseeto]

Helped in my understanding thank you

[@sina1234m]

Vielen Dank, das war richtig gut erklärt!!

[@Kaffeetasse9]

danke! endlich mal ein verständliches video :D

[@ChinchillaHappyLife]

Very clear explaination! Big thanks

[@swativerma6983]

You are a life saver. Thank you

[@wahahacoke]

Best explanation ever!

[@sevodar]

finally i got it . thank uuuu . Allah bless you ❤

[@graemelaubach3106]

Very helpful, friend. Thank you muchly!

[@satyavadlamani9420]

Nicely explained. Thanks a lot! Is there a particular transcription factor used for this? Or a criterion for choosing the transcription factor?

[@henrikslab]

Good question! So the main criteria is that you have to use a strong transcrption factor which initiates transcription of your reporter gene. In that case here they used Gal4p transcription factor: link.springer.com/protocol/10.1385/1-59259-762-9:247

[@weightelk]

Awesome, thank you for the clear explanation

[@pallavikamble2931]

Very well explained!!!thank you

[@swayambasu8597]

Hey thanks a lot for this brilliant video but I have a doubt here.... we take 2 components of the transcription factor AD and DBD and bind the 2 proteins one to each.......how can we say the function of the 2 components does not get altered when one of the proteins is attached to it? Pl help me understand

[@kki5061]

Thank you. Wonderful explanation.

[@peerzadazubairahmad464]

clear very much

[@purplesloth94]

Thanks so much for this helpful video!

[@黃傑-v9j]

It’s very helpful, thank you so much.

[@popitrock20]

thank you so much, so clear and concise!

[@55prasenjit]

Excellent explanation ❤

[@bioxplaining_by_anuska]

Very nicely explained.

[@Ashu-hy7br]

Just amazing👍👍👍👍

[@suriethaesterhuizen1802]

This is a great explanation, thank you so much!

[@Jake_M_Warren]

wow that's a very elegant system. Does it mean then that the proteins need to be taken up into the nucleus in order to be tested, is there limitations in terms of size and charge?

[@YJ_167]

so helpful, ThankYOU

[@lillininaw]

Richtig gutes Video, vielen Dank!! 🙏🏼

[@henrikslab]

Freut mich wenns geholfen hat!

[@nagylottii]

Thank you so much, it helped me so much!

[@thathzu2961]

Thank you ❤

[@ponktiaddrita6274]

Thank you so much.

[@juliariordan6082]

Great video thanks!

[@sajasamy8891]

Well explained thanks ❤❤❤

[@b.a.sbadassugar5007]

Every other source i found doesn't mention anything about Gal4AD recruiting the polymerase, they just mention that transcription happens. Do you happen to have any sources that show that GAL4AD does indeed recruit the polymerase?

[@aliilchizadehkavgani6663]

very good explanation, thanks

[@vinodsinghbhojak7383]

amazing

[@qny1286]

clear explanation

[@lanaamin3605]

Thanks alot, it was very useful

[@subhankarsinha448]

Nice presentation, thank you, make more videos on rdt

[@killianpapail9324]

thank you very much bro

[@ariyanshuvo5256]

Perfect❤

[@shridirehsis8560]

Thanks man

[@achmadsyarifudin2976]

Easy to understand good job

[@Kaur_2343]

Thnx a lot sir...it helped me a lot in undrstanding this concpt💖💖💖

[@henrikslab]

Perfect!

[@zalim2productions]

Very clear thank you so much

[@Rosessansepine]

Thank you it's really clear !

[@suhailahmad4120]

Thank you

[@futurogelatina4395]

Thank you so much!

[@matevzkosutnik7573]

OK so i have 2 questions i hope im not to late 1. how can you bind a protein to DBD or AD 2. what are the usses of this also i very much like your channel

[@henrikslab]

Matevz Kosutnik thanks for your question 1. you synthesize a DNA fragment where both sequences are connected and encode a protein with is ´chimericˋ meaning a novel protein out of two individual proteins which are linked via peptide bonds. 2. you can test whether protein A is interacting with protein B (if so, you generate a reporter gene expression)

[@meghanadhbanavath8850]

Good explanation

[@VanClemme]

Hi nice video! Hab eine Frage Also dient dieses System lediglich für die Erkenntnis ob Protein a und Protein b interagieren?

[@henrikslab]

Ist im Normalfall wirklich ein Verfahren um Interaktionen von zwei Proteinen zu checken, ja!

[@londonberry2180]

Does the bacterial adenylate cyclase two-hybrid (BACTH) system also utilise this concept?

[@Biochem656]

Thanks a lot

[@cloudtheavegner1000]

RNA Polymerase binds to the promoter and transcription factors bind upstream of the promoter.

[@marinbelz3448]

GJ!

[@annarauchberger7815]

Thank you!!!

[@user-er6mu5se6m]

U r great

[@nekoschneko7145]

danke Bruder ich küss dein Auge fürs video

[@pablonomas]

love u

[@tanvirshovon8246]

Ooooooossaaaammm

[@matteobarca1050]

Thank you so much! I liked your video and it was at 666 likes, so sorry if i ruined it!

[@henrikslab]

You did not! The goal is definitely a 1000 likes :D

[@anupamunnikrishnan8727]

i love you

[@henrikslab]

You can discuss this with my girlfriend :D

[@ilknurargum3326]

Thank you so much, ths video helps me a lot!

[@deepakmalik3746]

Thank you

[@jacobkiriakos8321]

Thank you

[@dhivya2k474]

Thank you