Unfortunately, I will not be publishing the answer key to this video. It was created as a prelab assignment for students. By publishing the answers it would make the assignment irrelevant.
@HaneenReda-z2i19 күн бұрын
Thank you for vidio
@REBAHAmira25 күн бұрын
best video, thank you !
@ProfessorDrewCollop23 күн бұрын
Glad you liked it!
@MairaMirfathАй бұрын
Nice content quite helpful thank you for sharing the knowledge
@ProfessorDrewCollopАй бұрын
Glad it was helpful!
@مرتضىجبار-ه1لАй бұрын
Hello, I would like to contact you for a graduate research position
@ProfessorDrewCollopАй бұрын
I am honoured that you would consider me; however, I do not take on graduate students at Seneca Polytechnic.
@مرتضىجبار-ه1لАй бұрын
@ProfessorDrewCollop I want to know how to obtain DNA from bacteria using a PCR device
@nwalk4529Ай бұрын
great video thank you!
@ProfessorDrewCollopАй бұрын
Glad you liked it!
@ShivTeaАй бұрын
What do you mean by high quality chunks when it come to deciding on the samples you choose for the heart?
@ProfessorDrewCollopАй бұрын
When cutting an organ, such as the heart, for explant, you want to have a plan in place before cutting. Often, students can just start cutting up the organ creating a slimy goo, instead of a solid chunk of organ. For explant technique, I like to have defined edges to the chunks, as opposed to a liquid mash at the edge. With a defined edge, cells tend to migrate out from the chunk when they exit mitosis. If the cells do not migrate out and adhere to the dish, you will not be successful. Also, with explants, it is better to have a few really good chunks, than a dish covered in mashed up goo.
@yasinhatami4779Ай бұрын
Best, i love your videos❤
@ProfessorDrewCollopАй бұрын
Thanks. I’m happy to hear you enjoy them.
@harisonsang3800Ай бұрын
What's TAE?
@ProfessorDrewCollopАй бұрын
TAE is an acronym. T = Tris, A = Acetic Acid, E = EDTA. Tris is a strong base. It has as very high pH. The Acetic Acid is a weak acid. It has a low pH. Mix them together and it makes a buffer. A buffer stabilizes the pH, so it cannot go up too much or down too much. EDTA is a chemical that chelates divalent cations such as Mg^+2. Chelate = Binds to them so they cannot bind to anything else. Mg^+2 is a necessary cofactor for DNase, an enzyme that degrades DNA. No Mg^+2 = No DNase activity = Higher concentrations of DNA. Running electricity in water leads to electrolysis of H2O. H2O --> H+ & OH- The H+ ions can interact with the negatively charge DNA molecule and neutralize it. If the DNA is neutralized, it will not be pulled through the gel to the positive terminal. The buffer will bind to the H+ and OH- ions and buffer them, so they will not interact with the DNA.
@vijyandutta5325Ай бұрын
TAE (Tris acetate EDTA) is a buffer used for separation of Nucleic acids while doing electrophoresis
@biochemfusion2 ай бұрын
Fabulous respected sir
@ProfessorDrewCollopАй бұрын
So nice of you. Thanks
@mad1cajon2 ай бұрын
Can I ask what is the name of the filter? Orange 21?
@ProfessorDrewCollop2 ай бұрын
Not exactly sure the name of the filter. It is the amber filter that came with the IO Rodeo Large Blue LED Transilluminator. I believe the blue light is at 470 nm and the amber filters light to about 580 nm.
@mad1cajon2 ай бұрын
@@ProfessorDrewCollop Thank you, much appreciated.
@NKD_MaDlamini2 ай бұрын
Sooo cool
@sonu07912 ай бұрын
Good practical video
@aymentyba63352 ай бұрын
Can you please share the absorbance values obtained?
@ProfessorDrewCollop2 ай бұрын
Unfortunately, I will not be posting the Absorbance values. Students use this video to record down the % Transmittance and then they are calculated the Absorbances. If I post the Absorbance values, the students will not need to go through this part of the protocol. To calculated the Absorbance (A) from the % Transmittance (%T) use the following formula: A = 2 - log (%T)
@FLAtiringa2 ай бұрын
Thanks professor, I have some difficulties to correct my manual count in chamber vs automated in countess 3. Using countess I have double of the cell concentration compared with the manual count, how to correct it? Thanks
@ProfessorDrewCollop2 ай бұрын
Not knowing any other information, there would be no way for me to troubleshoot this. Are you diluting in Trypan Blue for both methods?
@khushisaini95292 ай бұрын
What is the concentration of stock solution of BSA
@ProfessorDrewCollop2 ай бұрын
For this experiment you need to use 98% purified BSA. We buy the powder, so you can make up whatever concentration you would like. I believe for this experiment I used a 200 ug/mL stock solution of BSA.
@sherazkhan-sz8zo3 ай бұрын
Wow what an interesting lecture for studying microbiology and other related subject best wishes ❤❤❤❤
@mei-lani98633 ай бұрын
thank you this helped with my labs!
@Srirak-k3b3 ай бұрын
what nanometer u have used for this protocol
@ProfessorDrewCollop3 ай бұрын
I don’t understand your question. Are you asking me what wavelength was used?
@Collins_Ossai3 ай бұрын
Thanks a lot for this wonderful education. Please can these cell be isolated from test tube broth cultures as well?
@maleeshapriyanjana76043 ай бұрын
very good explanation!
@alkhansasalih73743 ай бұрын
Thanks for excellent video, what the code or brand of trypan blue that fit the machine, please?. Or not especific trypan blue?
@ProfessorDrewCollop3 ай бұрын
There is no specific brand we use. We have use commercial products and made it up from powder. With either version, watch out for particles in the trypan blue. I find they will always appear given enough time. These particles can through off the reading from the Countess.
@VedikaAnkitaAglawe-g2x4 ай бұрын
Thanks a lot for replying
@VenkataSiddharthPendyala4 ай бұрын
Hi, just a question. Can this process be applied to any bacteria?
@ProfessorDrewCollop4 ай бұрын
I am not a microbiologist, so I cannot definitely say it will work on any bacteria. There are alternative protocols that do exist. The purpose of going through each of the steps was to explain the reason we use each of these techniques. The great thing about science, is that it is always evolving. Once I discover a better technique, I switch to it after I have tested it myself. As a result, the way I run protocols in the lab is completely different today than it was years ago. One caveat is if you are working in industry. If you have a protocol approved for production, it cannot be changed with out going through the approval process again. So it is very important to have the best protocol before submission.
@ahmedharga8584 ай бұрын
How much is it?
@ProfessorDrewCollop4 ай бұрын
How much is what? The Coulter Counter?
@abayomiruthtitilayo49225 ай бұрын
Can you do a video of growing normal edible egg from scratch in the lab. Like ivf you take the egg from the hen, but you growing the egg in the lab without fertilizing it.
@ProfessorDrewCollop4 ай бұрын
If the egg is not fertilized there will be nothing to grow. That is what an edible egg is, an unfertilized one.
@abayomiruthtitilayo49223 ай бұрын
@@ProfessorDrewCollop yes that's what I want. Edible eggs. Can you teach one how to grow it. 😇😇😇
@ProfessorDrewCollop3 ай бұрын
This would be a question to ask an egg farmer.
@abayomiruthtitilayo49223 ай бұрын
@@ProfessorDrewCollop I know, but I thought scientist now have a way to grow edible eggs that is not fertilized. Guess it's my imagination, I was just so interested in most of your videos. That's why asked if it's possible to have a edible egg in a lab without a hen. But since it's not possible, it's fine. Thank for responding back, I really appreciate.
@ProfessorDrewCollop3 ай бұрын
There is a process to grow edible meat in the lab now, without killing animals. Search for "Clean Meat" or "Cultured Meat" to get more inform. A process to replace the creation of an edible egg is not cost effective. Hens are cheap and not worth the effort.
@PP-wh1wy5 ай бұрын
What if the counter shows cell number or concentration per mL but total volume of cells in the tube is 0.5 mL ? Your cell count won’t be per 0.5mL ?
@ProfessorDrewCollop5 ай бұрын
Concentration is irrelevant to the volume of your solution. The volume is only important if you are trying to determine the amount of the solute in your solution. As an example, you could have 0.5 mL of a 1000 cell/mL sample. It does not matter that it is only 0.5 mL, the concentration will still be 1000 cells/mL. As the concentration is a fraction, it can be used as a conversion factor to determine how many cells are in your 0.5 mL sample. Mathematically, it would be 0.5 mL X 1000 cells/mL. The mL units cancel leaving you with 0.5 x 1000 cells = 500 cells. Therefore you have a 0.5 mL sample, with 500 cells in it, with a concentration of 1000 cells/mL. Hope this helps.
@PP-wh1wy4 ай бұрын
Thank you for the clarification. Indeed was helpful
@prosistutesandlesbians5 ай бұрын
i have got to drink that
@ProfessorDrewCollop5 ай бұрын
I would not advise you drink it after the Biuret solution has been added. It is highly basic and contains copper sulfate and potassium sodium tartrate.
@Dominoes05 ай бұрын
How does this method compare to miniprep, eg alkaline lysis? Thank you!
@ProfessorDrewCollop4 ай бұрын
The alkaline lysis technique is optimized for purification of plasmid DNA from bacteria. The alkaline environment keeps the DNA denatured. Bacterial DNA is supercoiled, so even when it is denature, the strands remain in close proximity to each other. The genomic DNA is bound to other macromolecules (proteins & lipids) and will precipitate out of solution during neutralization of the alkaline environment. Many of the kits you buy come with a filtration tube. This will filter out precipitated DNA and allow the plasmid DNA to run through the filter and into the lower collection tube after centrifugation.
@valeriaiarteaga46506 ай бұрын
thanks for your informative video! is it ok if I did not use a bunsen burner since I did not have access to one?
@ProfessorDrewCollop5 ай бұрын
The Bunsen burner is there to heat the air in the neck of the flask. Hot air rises, so the air in the flask will flow up and out, helping to prevent contaminates from floating down in to your flask. If you don't have one, you might just have some contamination issues, but hopefully you will get many sterile plates.
@mspreddi16 ай бұрын
there is no voice over the video, hard to understand what's being done. It is like kids playing with a toy. No use.
@ProfessorDrewCollop6 ай бұрын
You might want to check your audio, as there is voice over for 50% of the video. At the half way mark I state that I am just going to be running the samples for larger number of wavelengths and I stop the voice over at that point, as it would be superfluous to continue speaking.
@mspreddi16 ай бұрын
@@ProfessorDrewCollop Yes; indeed. Could you please share the plots of the final output, on x-y axis? Also, in practical terms, what characteristics can it measure in wastewater?
@ProfessorDrewCollop5 ай бұрын
Sorry, I cannot add the final graph to this video, as this is an activity I get my students to perform after watching the video. If you really wanted it, you could graph it yourself. Plot wavelength (nm) as your independent variable (horizonal axis) and Absorbance (no units) as your dependent variable (vertical axis). The Spectrophotometer measures absorbance of different wavelengths of light by a sample. As a Biologist, I do not work with waste water. I suppose you could analyze the turbidity of the water as a function of how many particles are in it. We have some Chemists and Chemical Engineers, within our department, that work with waste water. If you are really interested, I could put you in touch with them. The Spectrophotometer works best for a single known chemical, with a known wavelength of maximum absorbance.
@LeeFarmberg7 ай бұрын
Thank you so much for your video!
@simranbhangu54997 ай бұрын
Thanks for the video. Its very informative
@lxldigvijaylxl7 ай бұрын
Is there any simple method to test beta glucan
@ProfessorDrewCollop7 ай бұрын
Sorry, but I do not know of one off the top of my head.
@Nahhhbro137 ай бұрын
idk why i love watching this lol, it’s fascinating!!
@jonathana92367 ай бұрын
That was helpful
@kubrasivri29158 ай бұрын
I am currently doing a master's degree in the animal breeding department and I am also an agricultural engineer/zootechnician. And I am about to complete my master's degree by receiving a significant project scholarship in my country, which aims to manipulate the gender of chicks by injecting herbal and artificial materials with the in ovo injection method. I have provided long-term support to many projects using feed and animal materials on poultry. I am the first generation in my family to pursue higher education and I am looking for a PhD education with a scholarship. Due to my increasing interest in laboratory studies, I have also completed the laboratory veterinary health department and am in the internship phase. I am looking for an internship in a laboratory in the field of poultry nutrition or breeding for the summer term. I have a laboratory animals certificate and a B1 level English certificate. I followed some of your works on KZbin with interest and reached you. I live in Turkey and I do not want to leave the field in which I work with interest due to financial difficulties. I would be grateful if you could guide me. Best regards
@ProfessorDrewCollop8 ай бұрын
Sounds like you are passionate with what you do. That is so very important. Are you referring to the manipulation of the sex of the chicks? Gender is a social construct. Sounds like a very interesting project. What type of chemicals modify the sex of the chicks. They only gestate for 3 weeks. You must need to inject the eggs very early. How to you ensure sterility. I am unsure as to what kind of guidance you are looking for. I do not take PhD students, if that is what you are looking for. Happy to answer any more questions you might have.
@hiramzen8 ай бұрын
good day professor, why is the boiling necessary for the reduction of sugars to take place, what causes the color change in the tubes, and what are the reactions called in this test? i am currently researching for our chem project and there are some things we havent understood properly due to our own professor's poor teaching so i apologise for the lack of knowledge
@ProfessorDrewCollop8 ай бұрын
I am sure there must be ample resources to explain the reaction if you were to Google it. In short, the heat usually needed to open up the carbon ring in the sugars. In Bial's test, the sugar is first dehydrated (loses water molecules) to create a new compound. Pentoses create furfural, while hexoses create hydroxyfurfural. These products will react with Bial's reagent, turns it from a yellow solution to a coloured solution. Furfural will turn blue-green. Hydroxyfurfural will turn brown. The chemicals in Bial's reagent include orcinol, ferric chloride and hydrochloric acid.
@apinyasripijit92059 ай бұрын
Hi Sir, I would like to ask this will contaminate with RNA?
@ProfessorDrewCollop8 ай бұрын
If you are concerned about RNA contamination, you can treat the sample with RNase.
@minhdao34289 ай бұрын
It hurts to see that you lost 1 drop of precious DNA at 10:06.
@ProfessorDrewCollop9 ай бұрын
Astute observation.
@restrictedendonuclease43409 ай бұрын
Sir i want to perform some experiments on a muscle .... So i took out muscle from a chicken (not from embroy like after killing it) and i kept it in normal saline as a physiological solution and after adding a pinch of glucose ...will this setup of my will work and if it does how can i make muscles contract in vitro
@ProfessorDrewCollop9 ай бұрын
This experiment is performed to make cell lines. You need fresh tissue that is not contaminated. Adult tissue does not work great, as the cells are bound tightly together and the cells are not in a state of constant cell division, like in the developing embryo. You will then need to disaggregate the tissue into individual cells, if you want to make a cell line. Without a circulatory system, the solid tissue will die as a result of a lack of oxygen and other nutrients. If you want to stimulate the muscle to contract, that is a completely different experiment. You need to harvest the muscle and find the nerve that stimulates it. Then you can apply an electrical current to activate the muscle fibers. I believe I performed an experiment like this on frogs back in third year Physiology. That was a course that changed my life.
@nareshbarik53849 ай бұрын
Thank you
@funny117449 ай бұрын
What method would you use for bone marrow isolation from the femur of that small chicken embrio ? The femur is too small to use a needle to extract the bone marrow. What method are used ? Thanks
@ProfessorDrewCollop9 ай бұрын
I have no experience attempting to extract bone marrow from a chicken embryo. Sorry, I cannot help you on this one.
@funny117449 ай бұрын
how many days has the embryo ??
@ProfessorDrewCollop9 ай бұрын
I assume you are asking about the age of the embryo. Once a chicken egg is fertilized the embryo will hatch about 3 weeks later. The embryos we used for primary culture in the lab are 2-weeks past fertilization. The embryo in the videos is slightly more than 2-weeks old by a day or two.
@Darkokofi10 ай бұрын
Thank you
@magicalcatwizard10 ай бұрын
Would there be a difference in results if yogurt and milk were to be used?
@ProfessorDrewCollop10 ай бұрын
You could used different types of yogurt to in an attempt to see the relative levels of protein in them.
@kyeommieluvr11 ай бұрын
Hi Professor! I have a question. How did you dispose the egg contents after culturing? Do you have any references for that? Thank you.
@ProfessorDrewCollop11 ай бұрын
I believe you emailed me this question already. If not, everything is autoclaved when we are done with it. In terms of references, I don't really have one. The protocols tend to evolve semester to semester as I learn new things or experiments don't work.
@DistrictCapitol11 ай бұрын
Hi there, thank you so much for your informative video. Would you mind sharing your protocol for BHK cells on the Countess please? Many thanks.
@ProfessorDrewCollop11 ай бұрын
Are you looking for the protocol on how I load the hemocytometer, or are you interesting in my gating protocol for BHK cells?
@DistrictCapitol11 ай бұрын
Your gating protocol please. I have started setting my own gating protocol and have been comparing results to our manual counting method. I have no other means for comparison, and I contacted ThermoFisher but they didn't provide much support. Thank you. @@ProfessorDrewCollop
@ProfessorDrewCollop11 ай бұрын
I had the same issue with ThermoFisher. I contacted them to get their gating protocol, but they offered no support. I really don't understand why they could not hired someone to go through the most popular cell lines and create protocols. Here is the protocol I am using for BHK. Not sure if it is perfect, but it works for my needs. I optimized it using a Coulter Counter and a manual hemocytometer to compare. Within the Countess appropriate range, it seems give approximately the same count numbers.
@ProfessorDrewCollop11 ай бұрын
Sorry, the image would not add so here it is typed out. Count setting both checked for Auto FL Threshold and Auto Lighting Size Gating = 12 - 28 Contrast Gating = 0 - 255 Roundness Gating = 0 - 75 Let me know how it works for you. Good luck.
@DistrictCapitol11 ай бұрын
Thank you very much @@ProfessorDrewCollop I'll try it out asap. Many thanks!