Primary Culture of Chicken Embryos - Step 3: Dissection

[@Nahhhbro13]

idk why i love watching this lol, it’s fascinating!!

[@杨邦邦-o4g]

Thanks for sharing! I have another question though. Could you tell me how the hypothalamus is separated from the chicken embryo?

[@ProfessorDrewCollop]

Unfortunately, I do no have this knowledge or skill. The brain from the chicken embryo is very small and jelly like. I am not sure if isolating the hypothalamus would even be possible at this stage of development.

[@kyeommieluvr]

Hi Professor! I have a question. How did you dispose the egg contents after culturing? Do you have any references for that? Thank you.

[@ProfessorDrewCollop]

I believe you emailed me this question already. If not, everything is autoclaved when we are done with it. In terms of references, I don't really have one. The protocols tend to evolve semester to semester as I learn new things or experiments don't work.

[@shahzaibshahid8178]

You explained every step really well. I just have one question though. Can you guide, how can we extract aortic arch from chicken embryo????????

[@ProfessorDrewCollop]

Hi Shahzaib, that might be a bit of a challenge, as it is extremely small. The vascular coming off of the embryonic chicken heart is easy to spot when dissecting the heart. In fact, no scalpel is required to remove the heart. A tug on by the forceps on the vasculature is all that is required to extract the heart. Why are you looking to extract the aortic arch?

[@kyeommieluvr]

@@ProfessorDrewCollop Hi Professor! I have a question. How did you dispose the egg contents after culturing? Do you have any references for that? Thank you.