What was the use of the 255 number in the normalisation formula?
@RashadSaleh929 күн бұрын
Thanks very much.
@RashadSaleh929 күн бұрын
Amazing video thank you very much
@RashadSaleh929 күн бұрын
Thank you so much that was so very well explained and straight to the point.
@ahmetenginpzc14 күн бұрын
Thats perfect!!!
@JacksonSeibert15 күн бұрын
Truly well done, thank you for your insight!
@Ombrenoirs16 күн бұрын
Amazing, thank you so much!!!
@chandramoulikaponnam917420 күн бұрын
how to design primers if we want to add a tag to protein?
@samcumdi22 күн бұрын
My 6th grader asked me about Spectrophotometer, "what does it do and what is it used for?" Aside from telling him, ''It is a light-related instrument'' - I didn't have much to say about it. What better feedback when he returns from school😊. No School like KZbin, Thanks Katharine Hubbard.
@AlissonCruzRivas22 күн бұрын
TY SM , DOING GODS WORK
@drchauhanrps28 күн бұрын
Thank you for this great video! Very informative!
@sergiumitrea643729 күн бұрын
Hello from România, i havent 110 apple tree with agrobacterium tumefaciens, i remove all trees , after how much years i cant plant apple tree in that soil near 2meter to the tree how have agrobacterium. Thanks a lot. And how i cant clean the soil...thanks...a lot
@asarhhАй бұрын
Awesome explanation 🎉
@hellfrrАй бұрын
Thank you so much for posting your videos. Very very clear explanation, great for understanding and for easy review later. You're saving me a lot of time and confusion. You're a wonderful woman
@szxnvАй бұрын
:)
@rrobatАй бұрын
Great tutorial! And about the volume of the reaction? Do you think we should consider it?
@vidhiverma7306Ай бұрын
Please can you tell the tool to design this type of primers?
@shenya2057Ай бұрын
Thank you so much ma'am, this video really helped me a lot :)
@MarcusElonNesoncАй бұрын
Thankyoy
@Geezweez788Ай бұрын
This is Varsity stuff but How i wish KZbin was around when I was in high school 😢
@XinyunQiuАй бұрын
Here is a general structure of a primer combining these components: 5' - Leading Sequence - Restriction Site - Gene-Specific Sequence - 3' When designing primers for cloning, amplification, or mutagenesis, it's important to include specific sequences that ensure the primer's proper function. Below are the key components to consider when designing primers for these purposes: The primer above includes: Leading sequence: GCG (3 bases to enhance restriction enzyme efficiency) Restriction site: GAATTC (EcoRI) Gene-specific sequence: ATGACTGACTGACTGAC
@user-ce4li4fj6hАй бұрын
Thank you very much for your videos! They are very well structured and helpful (from a chemist who learns bio)
@ZahidKhyzerАй бұрын
very nice , can you please help tell , can i use this for Gems Spectrometer
@rotemzilberman20352 ай бұрын
I guess after watching this video I just HAVE TO subscribe to this channel
@evaramos73322 ай бұрын
You are adding in the reverse primer (when you write It 3'-5') the sequence of the MCS in 3'-5' but that sequence that you added is actually the MCS written in 5'-3' (i ve searched the sequence). So, do you have to change the order of the MCS into 3'-5', dont you?
@SARATHATHEVIUPM2 ай бұрын
AMAZING.. WONDERFUL CLEAR EXPLANATION
@Glacierlune2 ай бұрын
Just imagine if a cell had a cloraplast and a mitochondria. They would be passing oxygen atoms back and forth.
@katharinehubbard50432 ай бұрын
Plant cells have both!
@Glacierlune2 ай бұрын
@@katharinehubbard5043 Lol I knew that at one time. I wonder why I forgot. I usually don't forget many things about biology. (But I have had some meurcury poisoning that has took a lot of my cognitive functions away, healing now)
@anthonytan61432 ай бұрын
Incredible video thank you so much !!!
@xinyuyu67382 ай бұрын
You are my hero!
@SamsonAruna-pp2se2 ай бұрын
You are a life saver. You explanation made it look like a simple thing to know.
@ruthanasreemoe3 ай бұрын
Can I just use test tube instead of quivet
@katharinehubbard50433 ай бұрын
Test tubes might work but you’d have to be a lot more careful about positioning them - the readings you get will be influenced a lot by the curvature of the tube, so unless you are confident the tube is in exactly the right place each time your data might not be very useful. Cuvettes are square so have flat sides that you don’t have to be quite so careful about
@ruggerogabbrielli68313 ай бұрын
Did bacteriophages evolved not to have those palindromic sequences?
@arijitmukherjee58463 ай бұрын
Can you please explain how to choose the wavelength in the spectrophotometer for calculating absorbance
@domhnall64923 ай бұрын
I Lov it
@Huy-tu7mx3 ай бұрын
Thank you for sharing! Very informative! You're such an effective educator!
@dr.mohamedel-telbany8303 ай бұрын
Amazing 😍
@MarinaG-f8w3 ай бұрын
Hello, I'm writing a lab report involving chlorophyll determination but I'm having trouble finding a study that uses these exact conversion factors and formulas. Could you please send me the link of where it was taken from? Thanks!
@harithhazim4534 ай бұрын
When an expert teaches something, life becomes easy. Thank you!
@vitoriaaraujo30204 ай бұрын
This video is gonna save my live in genetic engineering. Thank you!
@mahmoudofficial-e5w4 ай бұрын
that perfect as hell you must get much much mre mybe if you make playlist according to lippincot or harper , will be ammzing
@akshathabanadka98084 ай бұрын
How it g/L equivalent to microgram/mL. Isn't it mg/L?
@eloisepearce79494 ай бұрын
Thank you so much! Please could I ask how you would estimate an Amax value based on the experimental data?
@christinaliu31584 ай бұрын
very good explanation,watched about 5min and then I know this is what I needed. Also saved another 2 or 3 vedios for my paper (pharmacology😢) Many thanks
@cemalltr33854 ай бұрын
one in all, everything we must know is here in the video, I appreciate you <3
@batcryalok4 ай бұрын
Great video. Thanks.
@mikaelaprim42735 ай бұрын
Katharine.God bless your mother
@dr.satyabratasahoo56445 ай бұрын
Nice
@LeonardoBoroni5 ай бұрын
you saved me with this amazing video: clear and complete! <3
@hoangquan59135 ай бұрын
Thanks for sharing these videos for free, it's super helpful for Biotechnology section in my National Biology Olympiad!