Plasmid Design - Promoters

[@Danishalig-v9j]

Hey, thank you very much for your series. Really you save me. I was thinking i chose wrong career but your video gave me another hope. I really wants to say thank you very much for such great work. You saved thousand of beginners.

[@gregsmith6756]

This is really neat. I am determined to learn this molecular biology. Thanks for the upload.

[@user-ce4li4fj6h]

Thank you very much for your videos! They are very well structured and helpful (from a chemist who learns bio)

[@laurarojas3283]

Muchas gracias profesora por explicar de forma tan clara. You're the best

[@abumohammed9659]

Please keep going you are gorgeous. Many thanks goes to to from form UEA Norwich!!!

[@axospyeyes281]

Hey, loved this series about plasmid design. I didn't quite grasp the concept of eukaryote promoters though... Could you maybe make a seperate more in depth video about those? would be greatly appreciated, coming from an aspiring biologist!

[@katharinehubbard5043]

Thanks - eukaryotic promoters are on my list to do!

[@ashwinnair4816]

@@katharinehubbard5043 thankss

[@zixinleaf6499]

so do I, I think the concept of the eukaryote promoters is difficult for me. Such many upstream factors. I want to learn more detailed information, Thanks!

[@overlander2771]

I love you!

[@abdoulayeassane2852]

How about promoter for viruses?

[@katharinehubbard5043]

Viruses are a lot more complicated! Some viral genes are expressed using a viral RNA polymerase, while other viruses exploit their host cells (either bacterial or eukaryotic) and express their genes using a host polymerase. It depends what virus you are interested as how it's promoter works/is structured. There is therefore a lot of variation in bacterial promoter structure and more than I can easily capture in a 15min video!

[@abdoulayeassane2852]

@@katharinehubbard5043 Thanks for your kind feedback. Let's say "cassava mosaic virus". It will be highly appreciated If you could share some good references with us, Thanks in advance

[@katharinehubbard5043]

@@abdoulayeassane2852 I've not worked on Cassava Mosaic Virus so am not an expert here! However, Cassava Mosaic Virus is a geminivirus which has a single stranded DNA genome. Like a lot of DNA viruses, geminiviruses don't encode their own RNA polymerase, but rely on the host (eukaryotic) polymerase for transcription. Because the genes are transcribed with a eukaryotic nuclear RNA polymerase, they have a similar promoter structure to eukaryotic genes - Figure 4 of this paper shows the presence of TATA box motifs in regulatory regions. Hope that helps! www.ncbi.nlm.nih.gov/pmc/articles/PMC6640248/

[@abdoulayeassane2852]

@@katharinehubbard5043 Thanks for your kind feedback