Plasmid design (bacterial expression vector)

[@adindasaraswati1382]

I wept watching your video. If you're reading this, you're God-sent! I'm doing my Master's in Biotechnology, whereas I was a Chemistry undergrad. I know both are related, but in chem, we only went into a little detail about plasmid design. I was having a hard time while doing my Master's because I had no idea what I was doing in the lab (theoretically), but now I've got it! Thank you :) For whoever is reading this, if you've arrived at this video and are trying to learn more, then you're doing ok! Don't get too anxious 'cause as long as you know that you never stopped learning.

[@fatcammal]

I am also a chemistry undergrad doing a biology masters, I feel your pain

[@yeganefeizi328]

Your teaching is so amazing 👏 thanks for your time ❤️

[@hoangquan5913]

Thanks for sharing these videos for free, it's super helpful for Biotechnology section in my National Biology Olympiad!

[@vimalsingh6917]

Thank you so much for your excellent teaching. This is extremely helpful to me as a post grad student with no prior knowledge of gene expression and plasmids

[@ladyalexander2003]

Any thoughts that plasmids mRNA doesn’t actually belong in the human body and why so many people got sick from mRNA plasmid based vaccines? Knowing how zoonotic disease is caused why did they think using sick animals proteins and heavy metal adjuvants and chemicals would prevent disease surely all that toxic concoction would cause it wouldn’t it? Like they did when they thought feeding began cows sick proteins and caused BSE and nvCJD in humans?

[@vitoriaaraujo3020]

This video is gonna save my live in genetic engineering. Thank you!

[@leoszilard7542]

thanks for explaining the need for using different RE on either side of the GoI, I was wondering how to avoid the plasmid ligating to itself, but this is the first video I've seen address that as a possibility

[@ernestdrake1433]

thank you for your video! helps so much when starting a biotechnology experiment with plasmids!

[@JasonLee_1991]

Thank you for your video! It saved me a lot when I started my new experiments using plasmids!

[@katharinehubbard5043]

Glad it helped!

[@santoshgawali3175]

Very nice explanation and presentation, Thank you for this video.

[@eliranamar8497]

Thank you for uploading these great videos and sharing your knowledge with us! Really amazing ❤

[@marwanhesham7407]

Amazing explanation! Thank you so much!

[@marthamartha674]

excellent teaching , thank you

[@dr.mohamedel-telbany830]

Amazing 😍

[@oluwatayophilolatunbosun2062]

very good and informative. thanks

[@yeganefeizi328]

I'm really happy to find you 🥰

[@jackjohnson4586]

Thank you for your videos, they have been a helpful refresher!

[@ahmetenginpzc]

Thats perfect!!!

[@solomonderese9311]

Shukran, asante sana

[@hastaluegoo4647]

Hello! great video and wonderful contents, lots of thanks!!! i had one question: if you just cut into the EcoRI site and not other one, risking to random cloning, is there also a risk of re-ligation of the plasmid w/o insert?

[@ahmadfares5243]

Nice and clear, I have question its look like to what you explained. The question us , how can you desugne à plasmid cector to produce anti bogies ZMapp in chineese hamster overy in stable , thank you so much manger

[@gregsmith6756]

Does the gene of interest need sticky ends if only one restriction enzyme is used?

[@yimmeepop7283]

Great video! Thank you so much! I really need this.

[@mdzakariamorshed6778]

Dear katharine can u please make a video on crispr cas 9?

[@abumohammed9659]

Amazing thank you !

[@نادرتوانگر]

Thank you for your videos. Why do you put your pen on the paper? could you please put it away! Thank you.

[@katharinehubbard5043]

Thanks for the feedback - will try and remember to put pens somewhere else as I can understand it might be annoying!

[@nellymakeleni6717]

Thank you so much for this!.

[@mrighakalra6273]

Mam ,are MCS / Multiple Cloning Sites artificially created or naturally present in a plasmid..??

[@katharinehubbard5043]

Multiple Cloning Sites are artificially created during the construction of the vector (plasmid) - multiple restriction site are introduced at the appropriate site in the plasmid (e.g. downstream of a promoter) to allow for cloning of inserts.

[@mrighakalra6273]

@@katharinehubbard5043 thanks a very lot , mam

[@ashwinnair4816]

thank youu

[@Ombrenoirs]

Amazing, thank you so much!!!