Restriction Enzymes

[@hellfrr]

Thank you so much for posting your videos. Very very clear explanation, great for understanding and for easy review later. You're saving me a lot of time and confusion. You're a wonderful woman

[@damlagumren888]

Thank you for all your support! Great video !!!

[@asarhh]

Awesome explanation 🎉

[@eliotthugh8894]

Another great video! Thank you!

[@xinyuyu6738]

You are my hero!

[@gto11520]

Great video !!

[@ruggerogabbrielli6831]

Did bacteriophages evolved not to have those palindromic sequences?

[@mdzakariamorshed6778]

really nice ,

[@RobertAndersch-j2g]

Hi is there wobble in the recognition of the restriction site

[@katharinehubbard5043]

Not usually - restriction sites are very specific so any variation in sequence will stop the site being recognised - this can be exploited in genetic engineering as if you want to add/remove a site you only need to modify a small number of base pairs

[@solomonderese9311]

I have a problem with your use of two different restriction enzymes to cut the palsmid and gene of interest, won't this affect the joining of sticky ends through hydrogen bonding before they are ligated?

[@katharinehubbard5043]

Using the two different enzymes allows for directional cloning. If you cut both ends with the same enzyme the insert has a 50% chance of going in backwards. The sticky ends will still work at each end with two different enzymes as long as the plasmid and insert use the right enzymes, but won’t work if the insert goes backwards as they won’t have the right hydrogen bonds (as you identify) - this gives you more control over your cloning. Hope that helps.

[@alhaeri1]

thanks

[@AmruMagdy]

Another great video! Thank you!