Affinity Chromatography Explained

Рет қаралды 35,091

Cube Biotech (Cube Biotech)

Күн бұрын

Пікірлер: 25

@apearson79 17 күн бұрын

A cute animation that does a good job of describing what is happening :)

@b26awesome Ай бұрын

Thank you! - A biomed student in biochem

@agoldendream 10 ай бұрын

studying for my MCAT thank you!

@CubeBiotech 10 ай бұрын

Good Luck !

@Dooshduhloosh Жыл бұрын

I do this In my lab with his-tag for bacterial pesticidal proteins. this explanation helps clear things up. if im not mistaken the reason we conduct dialysis buffer exchange after is to remove any excess imidazole from our target protein as it is toxic to the insects we test and may interfere.

@CubeBiotech Жыл бұрын

Glad that we could help. And yes this sounds reasonable. Normal Imidazole concentrations in His-tag elution buffers is 500µM. This almost certainly must be washed away first through dialysis for any downstream animal tests.

@safiraandeetasaree Жыл бұрын

Great video! Love the animation so muchh!

@CubeBiotech Жыл бұрын

Thank you very much as well :) Glad to see that the effort pays off.😊

@vanesa5275 5 ай бұрын

such a cute video and great explanation!! thank you

@CubeBiotech 4 ай бұрын

Thank you :)

@rolfandco.4766 6 ай бұрын

such a cool and interesting video! NEVER KNEW THIS!

@CubeBiotech 4 ай бұрын

Glad that we could help you there. Did you mean something specifically?

@rolfandco.4766 4 ай бұрын

@@CubeBiotech just the whole process in general! I had to do an oral presentation and this was one of the main topics!

@lucianehernandez2778 11 ай бұрын

So helpful!

@CubeBiotech 11 ай бұрын

Thank you :)

@beatrizcalasensedecampos6991 6 ай бұрын

Super cute and helpfull, thank you! ❤

@CubeBiotech 4 ай бұрын

Thank you :)

@056vatsalapandey3 4 ай бұрын

can someone explain me the affinity and specificity logic

@CubeBiotech 4 ай бұрын

It is a trade-off. Higher affinity means higher vield, but that usually comes with less purity/specificity. Because if you make it easier to bind, false targets also get a chance to bind. And in the other direction this applies as well. If you make it harder to bind - higher specificity, you get purer results but also less yield.