1) Adeno Associated Virus (AAV) - An Introduction

[@sangeethvaheesan3173]

Great video, very simple and to the point, makes researching further a simple process without complicating the process. Thankyou.

[@abmgood]

Thank you for your feedback!

[@kathyc3005]

Great explanation, thank you so much! I wish it was possible to mute the music.

[@abmgood]

Hi Kathy! Thanks for your comment! We do have closed-captioning if you wish to mute the music. Regarding the music sound level, we have taken your comment into consideration - our newer videos have less intrusive background music.

[@tinacole1450]

excellent

[@TheGanjam]

Excellent video. Many thanks. Could you please also add a video on how to quantify AAV by real time pcr step wise. Cheers ;))

[@abmgood]

Hi there, thanks for watching our video and commenting! We can certainly put your request on our list of future video plans - thanks for the suggestion!

[@孙梦茹-o9j]

Great video

[@izzaimran9419]

itd be really nice if there were subtitles for this video!

[@abmgood]

Hi Izza Imran, thanks for watching our video! If you click on the "CC" button (bottom right of the video), you should be able to switch on closed-captioning for the video (the button will appear with a red line underneath it). Thanks for your comment and hope you enjoyed the video!

[@goes.]

So is the transgene delivered in the adenovirus or the AAV? is the only genetic material in the AAV the rep and cap proteins?

[@abmgood]

Hi Gabriel, the video describes AAV as it was discovered and its interactions with Adenovirus. When we are looking at using packaged AAV virus carrying a transgene for transduction, the genetic material carried by AAV consists of the payload (typically a promoter and a transgene) flanked by the ITR sequences. The Rep and Cap proteins are expressed from another plasmid during packaging, but this plasmid does not get packaged into the AAV itself because it lacks the ITR regions which are only on the plasmid carrying the transgene. This provides an additional level of biosafety, requiring the packaging plasmids to be present in order for the virus to replicate.

[@xzaviormilton4111]

Can you do gene therapy with influenza a virus vector ?

[@aswang7890]

In the tropism slide, you verbally mention AAV 9 but did not describe its tropism.

[@abmgood]

You're right - sorry! We cover it in our AAV Knowledge Base though: www.abmgood.com/marketing/knowledge_base/Adeno_Associated_Virus_Introduction.php#TAS (See Table 1 - Table of AAV Serotypes and their respective Tropisms.)

[@aswang7890]

@@abmgood Thanks for the link. You have a typo on Parvoviruses in the Intro.

[@abmgood]

@@aswang7890 You're welcome, and thanks for catching that. We've fixed it now :)

[@bdhome2010]

I want to explore genetic engineering from basic to research level. Can you provide me a guideline?

[@abmgood]

If you're interested in exploring genetic engineering using CRISPR, we have a CRISPR knowledge base that starts off with introductory material (www.abmgood.com/marketing/knowledge_base/CRISPR_Cas9_Introduction.php) and later goes into more details regarding experimental design. We also have a CRISPR Crash Crouse (a 4-week online course) that's free to join at info.abmgood.com/crispr-crash-course, and the materials are geared towards prepping you to performing your CRISPR knockout experiment.

[@vitorboico7050]

Its very nice!!!

[@abmgood]

Hi there! Glad you enjoyed our video -- thanks for the comment! Let us know if you have any questions!

[@userou-ig1ze]

please mute music. please don't confuse shavers and microphones. Thx, the internets

[@abmgood]

Hi there, thanks for watching and leaving your feedback! Yes, this was an older video we produced and we have since taken your feedback and others' and improved our sound quality. For this particular video, you can also mute and turn on the captions for a slightly better experience.

[@mauriziocelentano7658]

The background music is annoying and covers the speaker's voice.

[@mjangtao]

great

[@abmgood]

Hi mjangtao--thanks for your comment! Glad you enjoyed the video!

[@NamasenITN]

Audio is suboptimal and English pronunciation is not always very clear.

[@dramirfarhadbahreini6477]

Waw I appreciate the luciferase enzyme!!!!!!! Dr Farhad Bahraini from Russia academy of medical sciences

[@GrantMag]

2831 Jaquelin Coves

[@LingCamillo-e9p]

Charity Grove

[@hxzllj]

Who else is here because the odd1sout

[@calebthepro2676]

Me