1) Cell Culture Tutorial - An Introduction

What is Cell Culture?
➜ Cell culture is an incredibly useful in vitro tool in cell biology research. In this technique, cells are removed from an organism and grown in suitable artificial conditions for study. Live cell culture enables the development of model systems for understanding physiological processes in the human body, mutagenesis, carcinogenesis, drug screening and development, and much more. In this video, we will give you an overview of the categories of cells (Primary vs. Established Cell Lines), how to establish suitable growth conditions for your cells, and the principles of cell culture lab practices.
For more in depth information on Cell Culture techniques and for a list of the sources used, please visit:
➜ Knowledge Base: info.abmgood.com/cell-culture...
Thank you to all of the amazing community contributions towards translating this video into other languages including:
➜ Spanish
(To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.)
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[@Tarudox]

this guy is a fantastic talker. his voice is so soothing

[@smleguizamon]

I love everything about this video. Great refresher, calming voice, excellent images. Thank you for creating this!

[@abmgood]

Thanks for watching! ;) Glad that you found the video helpful!

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[@abinublitariswafimawarid3803]

Thank you for uploading this video. Very helpful for me who work with bacterial cell for 7 years and now work with mammalian cell.

[@abmgood]

You're welcome! ;) Glad you found the video helpful!

[@riasatmahmood4276]

5 min video explained a week of lectures thank you very much!!

[@abmgood]

Thanks for watching! Feel free to contact us if you have any questions.

[@andreagutierrez6816]

Thank you for helping me understand what cell culture is. I’m currently reading the immortal life of Henrietta lacks and came up upon this term.

[@abmgood]

No problem! ;) Glad you found the video helpful!

[@nekto6201]

It's very short, but interesting. Explains such difficult things in a simple way

[@abmgood]

Thank you for watching! Glad you found the video helpful :)

[@mustafaozturk4271]

Amazing! Lucid and lucrative. Thanks a lot

[@abmgood]

Thanks for your nice comment, Mustafa. We're glad to hear that! :)

[@sumitajegatheesvaran]

The best cell culture overview video on KZbin.

[@abmgood]

Hi Sumita, thanks for watching our video! Glad you like it - please let us know if you have any questions!

[@svetlanababiy1310]

All necessary information in short video! Thank you! I feel myself as a professional after watching that 😅

[@e_daddymarttini5497]

wow, actually really liked this video lol. Wasnt distracted by anything at all. Well done :D

[@abmgood]

Hello there, Thanks for watching! Glad you found the video helpful! ;)

[@mantelumgamingloilol123]

This is going to be great for my stem fair project

[@deniz4261]

I was looking for these informations, thanks a lot :))

[@abmgood]

Glad to hear that you found our video helpful! Thanks for watching :)

[@ayafares3447]

Even after 7 years your video is the best!!

[@abmgood]

Thanks for watching! :)

[@asgharbeigi9718]

Fantastic. This video has perfectly made and explains everything in a short but productive manner. Thanks

[@abmgood]

Glad you enjoyed it!

[@bardacha100]

Absolutely great ! Many thanks for sharing

[@abmgood]

Thanks for watching!

[@nourdiab4005]

Amazing video ! thank you so much !

[@abmgood]

Hi Nour, you're welcome! Glad to see that you enjoyed our video :)

[@miloradvlaovic]

Informative, concise and illustrative.

[@abmgood]

Thanks for watching! :)

[@yunacho551]

I love this vedio! so interesting and easy to understand Thank you

[@abmgood]

Hi Yuna! Thanks for watching and leaving a comment - glad you enjoyed our video!

[@NamasteNepal55]

thank U for sharing,,....

[@user-lp5qe1tv7d]

Thanks❤

[@vickyshaw278]

Great video! Thanks~

[@abmgood]

We're happy you enjoyed the video, Vicky! Let us know if you have any questions or comments.

[@alisonferdinand7600]

Amazing intro video! Thank you!

[@abmgood]

Thanks for watching!

[@ayafares3447]

Thanks!

[@abmgood]

😀

[@zeynepturk9091]

Thanks for sharing this great video.

[@abmgood]

Thanks for watching!

[@farahhusnan2467]

Good video

[@SaarimandRayyan]

Hi.. Thanx for the introduction. I am working on cell lines production in Pakistan. Can you know exactly the abbreviation of SH- SY5Y stands for? These are neuroblastoma cell lines but I am looking for exact abbreviation.

[@aarooran19]

good basic introduction to cell culture shan v

[@udaradayarthna6379]

Thank you very much.

[@abmgood]

No problem! :) Thanks for watching!

[@funny11744]

Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?

[@sirapongweawseetong6382]

I love this Video

[@abmgood]

Thanks!! :D

[@qioqio5662]

Thank you for sharing~~

[@abmgood]

You are welcome! Thanks for watching.

[@mydiary6827]

Wow awesome video

[@abmgood]

Thank you! Glad you enjoyed it! Please let us know if you have any questions about the material. Or, you can read more at our knowledge base: goo.gl/OtCzAJ

[@dougo6883]

Does anyone know of any good cell culturing books they have experience with? looking to purchase one.

[@sayeedalhawy1665]

This is Great

[@abmgood]

Thanks for watching. Please check out other interesting videos on our channel too!

[@xxxu1557]

thanks a lot

[@abmgood]

You're welcome!

[@ummuhaunijamalmohamed5497]

wow Amazing

[@abmgood]

Thanks for watching!

[@089_maulanadzaki5]

Thanks

[@abmgood]

Thanks for watching!

[@mayankjain2961]

do you provide technical know how to establish tissue culture lab

[@popaalexandru3971]

Do the infected cells perform the same as their non infected counterparts? I mean, do immortalized cells maintain their function?

[@abmgood]

Hi Popa, thanks for leaving a comment! Yes, for most cases, immortalized cells hold the same phenotype and genotype to their parent tissue, meaning they retain their original function. This reason, along with their extended replicative capacity, is why immortalized cell lines are more suited for tissue culture studies than primary cells. Hope this helps!

[@sidraayub4864]

Which app did u use for making the vedio??

[@abmgood]

Hi Sidra! We use Adobe After Effects to create our videos.

[@drameerkhan1248]

in which cell mitosis is occur adult embroynic cell or well differentiated cell????

[@abmgood]

Hi Ameer, if you want to know the difference between embryonic and adult cell with regards to their proliferation, embryonic cells proliferate rapidly and differentiate into specialized cell types which can be categorized into three kinds: 1) Terminally differentiated cells eg. cardiac muscle cells that are no longer capable of cell division. (If lost, cannot be replaced via natural repair mechanism). 2) Adult differentiated cells kept in the G0 phase and divide as required for replenishing the dead cells such as skin fibroblasts, cells lining blood vessels and epithelial cells of most internal organs, such as the liver, pancreas, kidney, etc. Most cells belong to this category and some form of injury/need to repair cells induces these cells to divide. 3) The third category is pleuripotent stem cells (less differentiated cells) which include blood cells, epithelial cells of the skin and digestive tract lining which have short life spans and must be replaced by continual cell proliferation. These divide to produce cell line lineage-specific cell type and once they commit to a specific lineage, they do not divide anymore.

[@JasmineDCarter]

Cell culture the same as induction?

[@dmitripastushenko1039]

Bruder muss morgen darüber ein Referat halten aber ich verstehe Nichts. Danke Bruder

[@alternation9124]

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[@pixie_laluna]

What's the difference between cell culture and colony formation assay ? (I don't have background in biology) :(

[@abmgood]

Briefly, cell culture is the process in which cells are grown in controlled conditions outside of its native environment, whereas a colony formation assay is an in vitro cell survival assay based on the ability of single cells to grow into colonies. Please check our Knowledge Based for more information about cell culture and cell culture assays and techniques: info.abmgood.com/cell-culture

[@pixie_laluna]

@@abmgood Thank you ! I'll check your recommendation.

[@AL-si8lx]

hi can you please give me some examples of secondary cell lines ?

[@abmgood]

Hi Ajay, thanks for commenting and watching our video! When Primary Cells (cells which you get directly from tissue) are grown in vitro and use up all the available space or media for growth, they will stop proliferating. In order to keep the cells in the proliferation phase, we need to take some of these primary cells and grow them in a fresh dish - a process called sub-culturing. The resulting population of cells in this subculture are called secondary cell lines. So, there are as many examples of secondary cell lines as they are tissue/cell types in the body! If you want to learn more about cell culturing, we have an entire knowledge base here: goo.gl/3LR25m

[@AL-si8lx]

Applied Biological Materials - abm Please give me any 2 example of secondary cell lines, finite cell lines and infinite cell lines if possible. just the names

[@gracietam95]

Thanks so much ABM for the link! Finally, I came across into the very right website!

[@Daoro123]

So, DMEM, RPMI and F12 are defined media right? There is no need to supplement them with FBS correct?

[@abmgood]

Hi Diego! You’re correct in that DMEM, RPMI and F12 are chemically defined media. However, they are usually supplemented with FBS because FBS provides vital macromolecules, growth factors and immune molecules.

[@sophia3770]

i have a question, so is this the same thing as cloning? im curious

[@abmgood]

Hi Sophia, thanks for leaving a comment! Cell cloning is the process of getting a population of genetically identical daughter cells from one parent cell - if you are able to start cell culture from one cell in a dish (a common strategy to isolate a single cell is to make a very high dilution), the subsequent cells will all be identical clones of this original cell. This video is discussing cell culturing in general and not specifically about cell cloning technique. Cell culture is simply a technique to take cells from an organism and grow them in vitro (an artifical environment).

[@aijazali.junejo6195]

Please give protocol

[@abmgood]

Hi Aijazali, can you clarify what protocol you are referring to?

[@sanaeallam3817]

Thanks a lot can i contact you I have some questions please !

[@sanaeallam3817]

like I need to know what raw materials are needed at this stage to produce a highly effective antigen for vaccines?

[@abmgood]

Hi Sanae, Please contact us at [e-mail] with your project details and our tech. team will further assist you ;)

[@omstygomsty]

Perhaps this is a daft question but has anyone here cultured there owns cells in a homemade lab?

[@komalpavitrakar8339]

How to do that?

[@aftabshaukat9407]

where is the link

[@STEM671]

RETRACTÄBLE COSMATICS ; DÜE PRÖJECTS 7:44

[@danieloulhint7914]

Great video however you talk very fast. Slow down!!

[@prasanthchakrapani8105]

Thanks