A UHPLC/HPLC Method Development Strategy with Complementary Stationary Phases

In this seminar, we review the importance of chromatographic selectivity in RPLC from a theoretical and practical perspective and how this relates to analyte resolution for method development. With an understanding of selectivity, and using a variety of chromatographic data, we discuss phase design principles and how it is possible to introduce functionality to enhance selectivity through mechanisms such as hydrophobicity, π-π, dipole-dipole and shape selectivity interactions.

[@achyutjagdhane9683]

thanks sir , i got a crystal clear clarity on subject , very nice explanation , thanks a lot for beautiful guidance .