Primer Design: Important Considerations and Tips for Good Primer Design

[@abmgood]

Here are some helpful time stamps for our Primer Design video: 0:41 Primer Length 1:06 Considerations for Melting Temperature (Tm) and Annealing Temperature (Ta) 2:40 GC Content 3:16 Avoid Repeats in Your Primer Sequence 3:39 Avoid Complementary Sequences Within the Primer 4:06 Avoid Secondary Structures in Target Sequences 4:31 Summary 5:07 Avoid List

[@berndelignie8002]

Depends on what pcr as for taqman assays, you can easily go 15-25bp

[@bytesizebiotech]

What is the reason for no more than 3x GCs in the last 5 bps? I've never had an issue with doing so. I always read that it's "At least 3, and up to 5 base pairs"

[@abmgood]

The reason to prevent having more than 3x GCs is to lower the chance of self clamping on the 3' end. Thank you for sharing your success with our viewers!

[@tourismhotspots]

Nice informative video. Thanks.

[@polinaskrobova5636]

Hi! What secondary annealing means?

[@faanutk7861]

hairpin and selfdimer are secoundry annealing

[@MohammadMahmoud-ji1jc]

I have created arms PCR primer; might I perhaps email it for revision?

[@abmgood]

Yes of course! Please email your inquiry to [e-mail], and our tech team will look into it! :D

[@MohammadMahmoud-ji1jc]

@@abmgood thank you

[@elvisnnaemeka6722]

You should have used an example to buttress your points

[@abmgood]

Thank you for your comment! We will definitely think about that next time we are doing a similar content. :D

[@nurulainafandi4267]

What is meant by Tm of product?...what is meant by word product?????

[@alfomeg588]

melting temperature

[@bio366geethasankar7]

🙏🏻

[@tayyabanazim100]

❤