Two-Dimensional Gel Electrophoresis

[@MrMetalHead1100]

One of the best explanations Ive heard on youtube for any molecular biology technique. Great Job!

[@lavendar1358]

A youtube science teacher without an asian accent with a clear and concise explanation? I must be dreaming..

[@lukestephenson1168]

He realised there was a market and he is taking full advantage of it :D

[@nadinecampbell3717]

Love you. Just pure brilliance across such a vast field. Bravo!

[@angadmahanta8807]

the main objective of all the aklectures videos is to get all your basics and concepts clear

[@medinlab141]

How can you be always so clear? I love your videos!

[@RachySarahForver]

This man deserves my degree.

[@jitukumsa225]

Never ever stop making videos. Thank you so much.

[@rainajung185]

Omg your lectures are awesome! Ive been watching all the gel electrophoresis videos and its so clear and also in details i dnt have to waste all my time looking for info on the internet anymore. Thank you!!

[@vikcheban923]

What a clever way to distinguish between different types of proteins

[@astradaniels]

This is very helpful for helping me understand material in my molecular biotechnology class.

[@harshvardhansingh1240]

simply thank you, you are the best teacher in the universe, you make things so easy , your every vedio is amazing!!, thank you for making us so knowledgeable

[@LuisRomero-mj6td]

Thank you, it was a good explanation. Please keep working, there are so many people that need it.

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[@mekz_n3505]

you are an absolute legend man

[@arlsffa]

U have no idea how much You´ve been helping me !! Thank You soooo much ! Since I found you, I fell in love with Biochem again lol

[@raquelmorellkessler3029]

Amazing explanation I understand everything with your Videos!!

[@yaseminacar4329]

Thank you for this clear teaching. This video helped me for preparing my exam :)

[@Raka96876]

Which exam ?

[@yaseminacar4329]

@@Raka96876 Genomics and proteomics

[@Raka96876]

@@yaseminacar4329 Are you doing Ph.D in genomics ?

[@yaseminacar4329]

@@Raka96876 it was my undergraduate exam. I am doing PhD in molecular pharmacology

[@rishimakhanlal8905]

Best explanation of 2D-PAGE!!

[@alexandremondaini]

Congrtulations for your great work and easily understandable teaching !!!

[@rashi715]

very helful videos ... thank u ... simple explainations to tough topics....

[@thehindureturn2339]

Thank you very much sir 💝 My concept is clear Love from INDIA # BHARAT

[@jaydeepadhikari]

This is so awesomely exlained.. Thank you very much!!!

[@themussseee]

Wonderful lecture finally understood this

[@shijimasorce]

Thank you for this. You do a much better job at explaining this than my professor. Likewise with a few other videos you have made. I just subscribed and gave your videos a thumbs up. :)

[@pavitrabhat9978]

Very Helpful.. Thank you so much sir..

[@jiaronglin8693]

You Are the BEST! ALWAYS!

[@TwiSTeDBeAnS]

It's a technique of separation and visualization, but not used as a method of purification, as purification implies that the protein is in it's native conformation. SDS denatures all of the proteins present so that they could not retain any activity and lose their binding properties.

[@MisssCooCo]

Omg! You are a life saver! Thank you so much!

[@KingDanny1123]

Thank you so much! very clear explanation!

[@AKLECTURES]

tammy wong you're welcome Tammy :)

[@kathrinasalud3755]

I love the way you discuss.. by saying "We" hehe

[@naomimankrado8510]

Where will I be without your videos? Thank you !

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I ould have failed without this channel and the channelShomu's Biology

[@okbazahra6298]

You are the best , thank u so much , your videos are so so helpful :)

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+okba zahra hi:)

[@okbazahra6298]

+Felipee Rincon hello :)

[@xRay5454]

Excellent video

[@suerose8297]

thank you so much, because this video I really understand this topic.😊

[@AmalDhivaharSURBT]

This is brilliant. Thank you.

[@chaimataifi8799]

Very helpful , thanks a lot teacher.

[@hajard6828]

very helpful, thank you so much!

[@ridsbutterfly9850]

you made it too easy.. thanks

[@mahya8450]

It can Not be better, thanks a lot ❤

[@teeteebaby09]

You are AMAZING! THANK YOU!

[@jyotijadhav9830]

Super explanation sir

[@Ku7zu3]

Thank u this video very helpful

[@ennaidevadla]

I learned a lot. Thanks!

[@jeannezhou5949]

very clear, Thank you VERY MUCH.

[@adindakadar9239]

I love this. I love you. Thank youuuu!

[@kimbokjoo6817]

Respect ✊🏼

[@anythinggoes4588]

Create your own religion! This got to be scientifically religious the way you explain so clearly. Thanks

[@charlesndandala9009]

Thank you, useful.

[@marionmutundu6086]

very helpful... Thankyou

[@amanda_dela]

you saved me from my supervisor !

[@KYADA1NONLY]

Amazing!

[@user-Kufamed_student]

Great✨

[@superoxidedismutase5757]

wouldn't the SDS apply a strong negative charge on all the proteins and affect horizontal motion towards the negative end in the Isoelectric focusing component? Assuming both SDS-Page and Isoelectric focusing is occurring together.

[@gershonanim6072]

They r done seperately

[@bhawnakhanna8884]

Nope... they aren't been done together...first you do the isoelectric focusing and then SDS page

[@veliborcabarkapa4271]

excellent

[@purvanshivakil2531]

Thank you !!

[@MrMetalHead1100]

Im assuming after you run the 2D gel, you can then do a westernblot as you would with a typical SDS gel and then treat with antibodies to determine different post translational modifications of a protein for example?

[@anne8nOtrn]

thank you so muchhh!! :D

[@joannaelhaj3571]

Thank you very much for your video. I have a quick question, when the pI is added to the SDS page, will the mass and size increase as you’re going down the SDS page?

[@milicialina3193]

Are proteins taken out of the pH gradient gel to apply SDS or is this done inside the gel?

[@zazaza8917]

Yo, thamk you very much mister

[@user-kr7ww2gb3f]

Question (might be stupid but ok xD) : with the 2D gel electrophorisis can you find out how many polypeptides,from each protein, there are at your first solution? No right? You just separate them based on these two different properties

[@biochemical846]

i have a presentation on isoelectric focusing on tuesday. i face problems in some points i cannot understand immobilized ampholytes, 2D electrophoresis is type of isoelectric focusing capillary method and SDS are also part of it. how to load sample in gel can you explain me

[@marianavalenzuela2986]

Is this Native PAGE electrophoresis?

[@aritradas4558]

But how do we separate those proteins which may have the possibility of having the same PI value as well as the same molecular mass. I.e they can't be distinguished by 2d technique?? Please advise.

[@aravindvenkateswaran5294]

This is to check stream purity. For separation, I would recommend using them in chromatography and looking at affinity chromatography in particular.

[@tenzlha]

can you reverse the steps and perform mass separation first and PI separation second. Why or why not?

[@thebro4860]

You can´t IP separation must be done first, because it separes based on the IP so you want the protein to be on its natural form and with it´s natural charge (+ or-), once you do SDS you denaturate proteins wich makes them to lose their dimensional conformation, they are long chains and SDS wich is negative binds to them, giving all proteins a negative charge and thats why now you can separate them ut of their size instead due att all of them have the same charge, wich is negative. So IP first then SDS always.

[@muhammadahmad-ft7fv]

nice done (Y)

[@sylvestermungombe824]

So those people who disliked this video think they can make a better lol

[@prasannaarun03]

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[@Aldream]

i still dont get it

[@kooroshesbati8761]

I wonder how you denature proteins after isoelectric focusing to work in SDS-page, if you take gel from first step the proteins are in their isoelectric point and wont move.

[@reinieradema6143]

holy this guys talks faaaaaast...

[@shubhragupta187]

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