Thank you so much, sir. Your pace of teaching is spot on. At no point does it feel rushed, yet I feel engaged and interested at all times. Keep up with the good work!
@gappythegoat53974 жыл бұрын
I like this professor, he's better than my teachers, and more fun.
@ss_jacko Жыл бұрын
Ok
@GameUnCrafter11 ай бұрын
Starting a new job in biochemistry and have only done this once. I appreciate the video.
@zillenoor99067 ай бұрын
i always avoided you due to your channel logo, it always teased me
@gcu33544 ай бұрын
😂😂😂😂
@lemmon-up4er3 жыл бұрын
Man 3 biochemistry an 3 organic medicinal Chem classes later I've never hear what I did so well...thanks Dave you explained its use so mater of fact. I had instructors from France, India, and China and they d just give equipment and show you how to operate it but not how it's operating.
@dk_mrkz7 ай бұрын
r u taking both biochem and medicinal chem? how r u?
@csodhi99694 жыл бұрын
Ah Professor Dave... The Jesus of Science, WAIT HE SHAVED
@GRosa4 жыл бұрын
Jesus shaves, hallelujah! 🙌🏻😎
@junkaccount25354 жыл бұрын
Went from Professor Dave to Professor Daddy overnight. Kinda looks like a buffer penguinz0
@hamzairaqi58832 жыл бұрын
The best teacher ever ❤️
@erzas55610 ай бұрын
Thank you so much for this clear explanation. ❤
@andrewc99984 жыл бұрын
We did gel electrophoresis in my first freshman biology lab.
@GRosa4 жыл бұрын
Neat, was always curious about how it worked. 🧬🧫👏🏻
@kimangerson3865 Жыл бұрын
hi professor dave! i noticed a mistake at ~1:50...the cathode is actually positively charged and the anode is negatively charged! you have this reversed in your explanation!
@IntellicastFacts Жыл бұрын
Common misconception… Anions are negative, and cations are positive. However, in gel electrophoresis, the cathode is negatively charged, while the anode is positively charged. (BIOLOGY) You are, I believe, referring to a galvanic cell. In a galvanic cell, anodes are negative and cathodes are positive. (PHYSICS)
@IntellicastFacts Жыл бұрын
Nothing switched, it’s just that there are different perspectives in the two subjects (physics and biology). The SURFACE of an anode is positive and the SURFACE of a cathode is NEGATIVE. However, the CORE of the anode is negative and the CORE of the cathode is positive.
@dennisjohnson8753 Жыл бұрын
Very good explanation
@poopoo95564 жыл бұрын
thank you dave
@GhostOfPerona4 жыл бұрын
I did this once in a school excursion. We used our own dna for this.
@mpumelelomandyoli25392 жыл бұрын
us too
@racheline_nya4 жыл бұрын
last time i was this early, he did not know a lot about the science stuff
@steve-vt5zu4 жыл бұрын
Your videos make everything make sense!! The intro still kills me
@iloveacademicsandalsomygir11312 жыл бұрын
i love professor dave
@ss_jacko Жыл бұрын
Tysm
@elozJ11024 жыл бұрын
Excellent video...
@imenebenamira28514 жыл бұрын
you are just awesome; the lesson was so clear i can't thank you enough and i ask you to keep on doing video in molecular biology thanks a lot
@joshmemarrynagdey16392 жыл бұрын
Very Very helpful. Quick understanding
@ss_jacko Жыл бұрын
That's what I do best😉
@sciencenerd76392 жыл бұрын
brings back fond memories from biochem lab
@zaid19904 жыл бұрын
New look 😍😍
@redhammer92736 ай бұрын
Omg it's professor Dave ❤
@LongDrive-ze9jw4 жыл бұрын
The professor is quite smart. 🤯
@Max-cn6sl3 жыл бұрын
Good shit, Dr. Dave!
@jananishambhasivam79524 жыл бұрын
You are awesome sir... Tku soooo much.
@KristoffDoe4 жыл бұрын
Professor Dave: "this technique can also be used to separate mixtures of proteins according to electrical charge" SDS-PAGE: "Am I a joke to you?..."
@atsuserlom12583 жыл бұрын
That’s very true, any objections to that?
@flookd55162 жыл бұрын
Non-denaturing PAGE.
@warrenterzol57254 жыл бұрын
Shouldn't the anode be the negatively charged electrode ?
@zhousiling68644 жыл бұрын
Because there's an electric charge applied, the anode is the positively charged electrode :)
@arihansen55562 жыл бұрын
Its called the anode because its where anions move, its unrelated to the charge, it would make sense to be cathode as its cationic but its where anions move so we call it an anode, similarly cations move to the cathode
@aidaht1 Жыл бұрын
Was studying for my exams and suddenly saw this video, it's great!! Your channel and videos are amazing, thanks for making these contents❤️🔥 Keep striving!
@chekein4 жыл бұрын
Where were you a few days ago I just had a test question on this 😣
@rahulsachdeva2728 Жыл бұрын
Great and precise
@ss_jacko Жыл бұрын
One of my techniques😉
@PromiseChiamaka-i5d Жыл бұрын
Thank you sir
@hamdimohammed25413 жыл бұрын
A video about 'Gene fingerprinting' or more about Southern Blotting would be much appreciated, thank you.
@mr.mohsen6069 Жыл бұрын
An accurate and interesting explanation, thank you
@steptimusheap88604 жыл бұрын
I remember this one from Bio!
@PIERRETMENDY-xp3bn4 жыл бұрын
Beautiful ❤️ lectures I'm going for an exams on Biochemistry on this topic part 〽️ Gel electrophoresis! More lectures on it
@Science_Through_Art4 жыл бұрын
Sir plz can you make a video on drift velocity because it's a quantum phenomenon
@ss_jacko Жыл бұрын
Ok I will
@chely952 ай бұрын
Can you run through interpreting the bands
@davidsmithsmith56794 жыл бұрын
Can we make a plane, boat , car race around the world where both teams have to build them from scratch.... Flat earthers vs Normal people.. who will win? P.S this is not a plan to drown flat earthers XD
@mittalpatel8433 жыл бұрын
How to interpret the gel results? How to know which plasmid has insert or self ligated or why are some results showing RNA?
@flookd55162 жыл бұрын
3:05 & 3:15. The left hand lane contains DNA of fixed sizes to use as a scale. In checking correct insertion you can run a lane with the unaltered plasmid as a comparison or (better) digest some of the new plasmid and check to see if the right size insert appears.
@lmtrevino74 жыл бұрын
Wow can you make a flow cytometry or maybe DNA RNA Extraction or PCR
@flookd55162 жыл бұрын
You can extract DNA/RNA from a cut-out section of an agarose gel by dissolving it.
@vaibhavkumarkawale29924 жыл бұрын
Sir iam big fan of you
@ss_jacko Жыл бұрын
ty
@clone544 жыл бұрын
But bigger DNA also has a bigger negative charge, and should have a bigger force acted on it. Why doesn't it make it move farther?
@A360-j5w4 жыл бұрын
Even though it has bigger negative charge it will have more molecular weight.
@sjholmesbrown4 жыл бұрын
Because the negative charges are evenly spaced along the DNA backbone, and the number of charges are proportional to the length, the DNA receives a very even force per unit length. As a result, the migration is affected mainly by the difficulty in sieving through the gel pores. Where the charge of the molecule has a big effect is in Native-PAGE protein electrophoresis, since a heavily charged protein will be pulled through faster. In SDS-PAGE (the first picture Dave showed with the bright blue sample wells was SDS-PAGE) the protein is solvated by negatively charged detergent, so it acts mainly on size.
@nooraaliraq4 жыл бұрын
I am going to have exam in martin physical pharmacy next month especially in biotechnology. Chapter ...... i need help in this exam ... can any one tell me what online lectures will help me ... 🙏 😢😢
@shayekhabedin44624 жыл бұрын
LEGEND
@kittiesstan04 жыл бұрын
Wish my teachers were as good as u 😭
@ss_jacko Жыл бұрын
haha
@Hyumanity3 жыл бұрын
Very cool - thank you :)
@ss_jacko Жыл бұрын
Np
@westwoodoralsurgerydentalgroup2 ай бұрын
amazing
@MR-zr9se3 жыл бұрын
the location of plasmid DNA in agarose gel can be visualized by ethidium bromide or bromophenol blue??!
@MR-zr9se3 жыл бұрын
What’s the difference between them?
@flookd55162 жыл бұрын
EtBr will (with UV) but bromophenol blue is just a marker dye that tells you the gel is running OK.
@jeffhambu9106 Жыл бұрын
Mi recommend yu watchem emia too🤝❤
@yashmehan39444 жыл бұрын
i am the first viewer! yay!
@amalitad64592 жыл бұрын
Isn't anode is negatively charged?
@ordinaryindividual62462 жыл бұрын
Nope, the positive anode attracts anions toward it, therefore it is a positively charged electrode :)
@tas91609 ай бұрын
Isn’t anode (-) and cathode (+)?
@fereshtehm227 ай бұрын
I think you confused anion with anode.
@rajmoulighosh53755 ай бұрын
No cathode is negative and anode is positive. Exactly the opposite charge of what cations and anions are
@KLSanthosh-m3qАй бұрын
100 th comment ❤ professor 🎉
@pericycle Жыл бұрын
Thomas and his friend inspired intro......
@razasyed575 Жыл бұрын
all hail professor martin davidson
@Blubb50004 жыл бұрын
I know some of the words.
@chittibabu65334 жыл бұрын
Sir I'm not getting video s in gmail
@ProfessorDaveExplains4 жыл бұрын
The videos are on KZbin, bud. If you subscribe and click the bell for notifications, it will tell you when I upload.
@LFG-28 Жыл бұрын
👍🏻
@lincolnoliveira30413 жыл бұрын
Ltc
@krishaangela85524 жыл бұрын
omg the hairrrrrr
@skooterbooters4 жыл бұрын
1.25 speed
@anig.34624 жыл бұрын
I hated high school biotechnology thanks to the psychotic teacher I had who sprayed us with di water :(
@kimbokjoo68173 жыл бұрын
Who sings the intro every time
@saitokajaki23083 жыл бұрын
How do we know if the food product is GMOs or not by using gel electrophoresis?