SDS-Polyacrylamide Gel Electrophoresis

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[@保禄-d9c]

about F=Fv：Because friction is more complicated, it is usually some empirical formula. In the past, the friction force you were in contact with usually referred to dry friction between solids. If you consider that the object is moving in air or liquid, the measurement will find that the friction force is related to the speed. It is generally considered that at high speed, the friction force is proportional to the square of the speed, and when moving slowly, the friction force is approximately linear with the speed.

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you can follow mine

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Done!

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thank you

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[@fatmamahmoud2689]

Thank you for the simple explanation and the clear diagrams. I was in a bad need to understand that type of gel electrophoresis.

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Loved the way you explain..every point. thanks for explaining it 👍 so well

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your lecture is so on point. Thank you so much for being helpful to me!

[@AKLECTURES]

Lisa Touyon you're welcome Lisa, glad to be of help :)

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one of the beautiful lectures i listened..thanks dear andrey

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Drhari Kartha Thanks a ton! :)

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The best explanation EVER! Thank you :)

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Fantastic explanation🎉🎉❤

[@BBC932]

That is a very good explanation. Many thanks

[@weakcandlelight7793]

great video! Please continue to make more.

[@lauradumo]

Best explanation ever, thank you so so much!

[@PillsHere222]

Not be a nerd, but SDS is missing one more carbon in the video (Dodecyl = 12). I only see 11 carbons in the drawing. I appreciate your work, and needed this very much.

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You are amazing! Thanks for your explanations

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[@AKLECTURES]

Thanks Samantha!

[@kathypychova1137]

thank you, finally understood physics :D and ofcourse SDS-PAGE... your lectures are great!

[@AKLECTURES]

+Kathy Pychova you're welcome Kathy :)

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what a good presentation. keep it up

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Very beneficial lecture. Thanks so much....

[@kikiignatiadis9212]

This was informative and awesome. Thank you!

[@alexrytikoff9627]

Great explanation. Little review of physics was helpful

[@AKLECTURES]

Alex Rytikoff Thanks Alex, always like to use physics when applicable! :-)

[@tayzarlinn7619]

AK LECTURESplease show me the link of denatured polyacrylamide gel DNA .

[@AKLECTURES]

tay zar linn google it.

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Great explanation!

[@jhondelv8891]

Overall good lecture....... 8:20

[@bacanimichael]

great explanation

[@Artas1984]

He is right about the electric field being constant, BUT! Actually the voltage is not constant! In order to maintain a constant electric current, which generates ''almost'' constant velocity, the gel-electrophoresis machine increases the voltage during the process in order to resist the ever increasing impedance values inside the medium (anyone doing gel-electrophoresis can see that). Actually during the migration the voltage more than doubles! Does not matter in the end... Excellent video anyway!

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Excellent! Well done!

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simply outstanding

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Accidentally today is the birthday of this video 😂

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just what i needed ! thank you very much !! ^_^

[@AKLECTURES]

adiga202 You're very welcome! :)

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[@AKLECTURES]

lovelyy arora thank you! :-)

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thank you for this video.

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your videos always help me, but I only need you to zoom in the board, so tht we can see whts written

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brilliant lecture!

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f begum thanks! :)

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Dope lecture

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totally awesome..thnks very much..

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Hands off

[@abbierieder5807]

How is the equation F_f = f*v derived? I know F_f = mu*Fn but not sure how you're getting that derivation.

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Thank you very much ...

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your the best

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Great! Thanks!

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You are the god

[@deepikadav]

understood it very well in just the moment :D :)

[@khaledcampa9593]

Thank you!

[@ProfoundAscension]

Great video, you are very easy to understand. Does SDS page disrupt disulfide bonds?

[@jonvonstein3390]

+M Abdul You are breaking the sulfide bonds with DTT, BME, or TCEP before you put it in the well.

[@ridsbutterfly9850]

Thanks a lot Sir..

[@johnwaynard1506]

It occurs to me that the structure formula of SDS written on the white board is incorrect (one "CH2" is missing in the dodecyl fatty acid)... But otherwise: great explaination!

[@lourdeskottakali434]

Thankyou

[@Gooch_cruiser]

I always thought the reason ads page was due to size not charge was because the charge to mass ratio for all proteins is the same with SDS. 10 amino acids = every other amino acid gets sds/ = arbitrary net charge of 5. Divide that by 10 = 1/2. 30 amino acid... 15 charge.. 15/30 is 1/2 .Thus all proteins have the same exact charge/mass ratio.

[@SforScience]

sds add charge uniformly to each and every amino acid so that they can separate based on their charge ratio

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thank you so much for these videos!!!!!! thank you thank you thank you thank you.... oh yeah... THANK YOU :)

[@namra12345]

Thanks a lot sir

[@Soriyou3]

Thanks for a great video. I have one question. How is the force of friction = f*velocity? Isn't frictional coefficient dimensionless so the unit does not come out to be Newtons?

[@moishie20]

thanks men

[@moririn9923]

can we obtain the separated protein from the gel

[@abomashhoor583]

amazinnnnng. can i ask a tow question: 1. whats the deference between 6x and 2x loading dye and which i should use ? 2. which voltage is optimal to run one caste of gel, i usually use 65 v. ?

[@GabrielThomassen]

the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.

[@GabrielThomassen]

the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.

[@maxwellnjati1756]

The friction comes from the gel?

[@mahermohammed7448]

plz i have a question , would you tell me the nature of denaturing take place by SDS on protein which doesnt effect on the conformation of protein

[@stephanieangela470]

Hi I'm using potassium sulphate buffer in my sample. can it works with it or does it has to be tris HCl? thank you

[@finnvanschijndel7422]

thnx homie:)

[@lirenseparediano3504]

Try to explain in many languages, for example in spanish... Nice videos !!

[@Lejonet120]

try to teach enlish :)

[@junyoun4470]

Can anyone please explain Why frictional force is => frictional constant(f) X velocity (v)? Far as i know Frictional force is equal to frictional constant(f) X Normal Force (N)

[@herrmanselcher1632]

Thank you so much

[@amirhosseinshafieian3951]

I think there is a problem, the coefficient of friction must be without dimension, while here you when you multiply f with velocity it does not make sense, would please clarify it???!!!!!

[@Wanderer789-v8o]

What is the role of mercaptoethanol???. Plz reply

[@menassies3224]

Farida Chy the presence of mercaptoethanol disrupts the disulfide bridge bond found in the protein when mercaptoethanol is not present the disulfide bond in protiens stay intact hope that was helpful

[@lilna7444]

Mercaptoethanol is a reducing agent that cuts disulfide bonds within proteins in order to disrupt the 3-dimensional shape of proteins, a process that is called "denaturation".

[@andreabanda3605]

Can someone explain how I can use this procedure to determine which membrane proteins are integral vs peripheral?

[@Luvmiami1]

What a boss

[@aditilad3173]

Please make a video on Native page