Agar series video 2 managing condensation

[@wdinkins281]

I’m sure this is great info and I picked up something from watching the video, but I can’t hear you over the music. Hardly anything at all.

[@mushroomcult]

Yah. I'm learning the video stuff. It takes a lot of time. I do more on TikTok

[@waynekaczmarek946]

Myself also

[@vazap8662]

That's a great explanation thanks. I'm going to test using my sous vide today, along with an electric air heater to warm up my sab. I have tried letting my plates vent off condensation before putting the lid on, inside my sab, they were visibly very clean but did get some contam. Not 100% sue that was the cause but I'm cautious aout doing this..

[@mrpeanutbutter6094]

This happen to me plenty of times, I wish I could able to share pictures of how condensation really effects bacteria on a Agar plates. You should wait when the agar haas cool down to 60-50 c. Then continue to pouring it to the agar plates. Another way is to let the lid open and wait for the steam to disappear. Do this right after the agar temperature has drop down to the desired temperature.

[@mushroomcult]

good tip. you can send pics on instagram or over my email. www.mushroomcult.net

[@mrpeanutbutter6094]

@@mushroomcult ok thanks for the link, I send it to one of your email listed in the website.

[@davids11131113]

Can you just put a well colonized grain of rice on an agar plate is that ok? Or should I try to just get a bit of mycelium off of it instead? I’ve got 8 rice bags almost fully colonized, and 10 agar plates I want to keep this strain going and looking for the best way.

[@mushroomcult]

Great idea. This is a great way to save a cultivar and to quality control your grain spawn. Make sure you use aseptic technique to transfer the piece of rice

[@davids11131113]

@@mushroomcult oh for sure I just got my North Spore NocBox 👍

[@rossanafurtado513]

Awesome! Gonna try for sure

[@mushroomcult]

Please do!

[@rossanafurtado513]

@@mushroomcult just did, it worked. Thank you! 🙏 😊

[@RobertViani]

What about pre warmed/heated plates or jars and adding agar via a self sealing port?

[@mushroomcult]

Warming the plates is a great idea. That will reduce condensation to some degree. I'm not sure that adding the auger through an injection Port will decrease condensation. It could increase risk of contamination depending on your technique

[@sporemuse]

Thanks for the helpful tips!! ⚛️

[@pnat_live]

So, my situation is that I watch this video late, lol. I just poured 20 plates a couple of days already. How can I fix the condensation for the moment?..NEW SUB!!

[@mushroomcult]

You can dump the water out when you use the plates. If you have a flow hood you can let them dry in the air flow. Also store the plates up side down.

[@benink5690]

Quick tip about sous vides, they are usually meant for 4-12 hour runs. It will get to exact temp 100%. But it takes a long time. Might be best to take from pressure cooker hot and put in sous vide to come DOWN to temp rather than up. obviously an ice bath would make this faster back n forth.

[@mushroomcult]

Exactly. The sous vide a is only for bring the temp down after sterilizing. It can hold the temperature longer if needed

[@mimitruong543]

HELP! So my plates are under a lamp for incubation and there is a layer of condensation on the lid. I've emptied out the water, but it's still condensing.

[@mushroomcult]

the issue is probably because the lamp is applying heat to the plates. most of the time there is no need to increase the temperature of the plates for incubation. normal room temperatures are perfectly fine. between 60f and 75f are perfectly fine.

[@mimitruong543]

@@mushroomcult ok so I don't have to incubate my inoculated plates? Thank you for replying quickly👍

[@jarrodsfight4204]

I am having an issue with my actual agar sweating, and it ruins everything. Some times it does sometimes it doesn't in the same batch, and ideas on stopping this?

[@mushroomcult]

Are you getting a firm gel? When does the sweating occur? What is your recipe?

[@dchaney954]

This is no frills straight education I love it

[@mushroomcult]

That is my goal

[@maxplank1196]

Please turn down the music

[@Arlet11369]

Could also make sure your plates aren't cold when pouring.

[@mushroomcult]

Good point. It's all about temperature

[@MyHealthyBirds]

Hi do we need sterilise that plastic petri dishes before agar?

[@mushroomcult]

no. they should be sterile before you open them

[@jamesnutt898]

Top self information my friend, I have been doing something very similar to what you are doing and the results are perfect 🥰💞

[@mushroomcult]

Thanks for your feedback!

[@Pamutsetse]

Bro thank you for the video. Keep up the good work. I’m going to try it in 30 minutes.

[@mushroomcult]

Did it work?

[@FreshfromtheFarmFungi]

awesome work! Love the idea - is there a maximum limit to the volume of the water heater? Also, I recommend getting a laser thermometer that way you can see the temperature decrease in the agar without contaminating it - MUSHLOVE

[@mushroomcult]

I will plan on getting a laser thermometer so I don't need to guess any more. Im sure there is a maximum water volume but it is not in the produce instructions i may run a trial to find the max. the container i used in the video preheats to 124°f in minutes so i bet it can handle a pretty large volume.

[@metlwrker4285]

Your music drowns out your very soft spoken words so much that I can"t follow what you're saying

[@themyceliumnetwork]

i love your videos but can never hear what you are saying. my speakers are at 100% when I only have them at 5% to 10% on other videos

[@mushroomcult]

I need an producer and editor.

[@mushroomcult]

I need a producer and editor

[@danielboomers]

why is there music so loud playing?

[@mushroomcult]

Production Error

[@RealMTBAddict]

Vertical video?

[@mushroomcult]

i do what i can

[@ISawYouMushroomHead]

Thanks for the video

[@mushroomcult]

You bet

[@L19-o7r]

"Auger"

[@mushroomcult]

i do what i want.

[@infectedpuddle1638]

He has a point. What you are pronouncing is a tool used to dig holes in the ground for fence post, decks and houses.

[@realkingofantarctica]

Senjougahara best girl

[@mushroomcult]

good

[@search620]

why the music soooooo fucking loud?

[@arseybotter]

apppaling sound quality. This was made in 2019... they made a better job of sound in 1930.

[@mushroomcult]

do you know how to fix this? im just learning this AV stuff. thanks

[@shainemaine1268]

@@mushroomcult It almost sounds like you've "scooped" your mic's EQ (turned the mid all the way down with treb/bass all the way up)... also maybe kill the tunes when you're talking, it just makes it worse. I really need this info, but could barely understand it... How are you recording audio?

[@shainemaine1268]

Alright this is just impossible.... did you watch this before you uploaded?

[@mushroomcult]

yes . it sounded different and louder before uploading. i dont see a way to standardize or change the volume.

[@nazlisuleyman4535]

Dude just get to the point, you could have made this video in 30 seconds, I didn’t come for the definition of condensation!!!!

[@mushroomcult]

thanks for the comment! I am trying to lead people in how to think about condensation rather than just prescribe what to do. I am always trying to keep my videos as short as possible. i get frustrated when a 20 min video could have been 2 minutes. I will try harder to condence. thanks please let me know what you don't like about the other videos too.

[@shainemaine1268]

Yeah we get that, but go ahead and assume people know what condensation is... If they cant do a oogle search on a term they were taught in third grade, this probably isn't the hobby for them anyhow.

[@jamesbumgardner1469]

@@shainemaine1268 why do a Google search when it's all right here in a short 7min video 😁 the mushroom community makes the weed community look like the friendliest place on the planet. So much elitism and condescension its nauseating.

[@nowirehangers2815]

Thank you. I heard you just FINE X

[@mushroomcult]

great. any questions?

[@ludahfang559]

What did you do edit on your phone? 👎

[@mushroomcult]

its a learning process. do you have any recomendations?

[@Germanboy567]

All these babies in the comments, Jesus

[@mushroomcult]

I appreciate the criticism. do you have any criticism of my other videos?

[@kkawa6533]

get a better mic, also background music is annoying. your voice is competing with the music

[@mushroomcult]

thankyou. i appreciate your observation and solution. i will be working on this. any other criticisms?

[@johnriperti3127]

Please remove this annoying music ! We can't hear you

[@mushroomcult]

Yah it's pretty bad isn't it.

[@johnriperti3127]

@@mushroomcult Still a very good and informative video. Thanks for that. It's easy to complaint. You are not the only one to do that.

[@jakereeves8172]

Invest in a real microphone bud. Otherwise good video!

[@mushroomcult]

thanks for the criticism. i will look into a good mic. thanks

[@bbhoustontx]

he has a soft voice .... piss off!