Sequencing Amino Acids by Proteolytic Cleavage

[@Toothfairy1018]

explained better than my professor. You're a life saver bro!! Thank you so much for your videos!

[@viktorholicek8201]

Haha I like how you start cracking up when you say there is only a 22% chance it will work!

[@wem3121]

I love all of your videos! I mean, recently everytime I want to revise any biology subject my first direction would be AK LECTURE to understand; and then I'd start reading the lesson of my teacher. THANK YOU very much.

[@akuchiha777]

Use ads and monetize ur videos sir , your a life saver

[@jessicastrohmeyer5786]

This man is the only reason I have any idea what is going on in biochem

[@ruaaissa1245]

I wish you were my biochemistry professor.

[@Stephanie-tx9mi]

you are amazing!! my biochem mark skyrocketed thanks to you

[@maddymc4648]

Very organized thought process. thanks!

[@sabaalshoubaki7095]

praying all next generation of biochemistry professors will be just like you

[@summertate8525]

Missed my Biochem lecture the day we went over this topic. This video really helped me! Thank you!

[@ivy_2828]

this is so helpful for my biochem module thank you😭

[@chengshenhansu6799]

you are amazing!!!!! can't survive in uni without you

[@simaralaboy1013]

I would be failing my BioChem class if it wasn't for you!!! LOVE LOVE LOVE!!!

[@frankaddeo6132]

Thank you so much for the contributions, your explanations are spot on. I appreciate your efforts!

[@ranevergarden6430]

you are actually my biochem prof

[@LUKE-eu8iv]

omg finally I am loving to hear you say "What that means is~" LOL

[@kareemjeiroudi1964]

Amazing explanation

[@cooliozhu]

Hello AK, thanks for your great lectures. If possible, can you step to the side at the end of your lectures to leave the board by itself so that we can screen capture the notes?

[@andreeanasca89]

thank you so much! I finally got it thanks to you!

[@andrewmurphy5403]

Great video. Thank you!

[@tinatina-tu4mb]

Methionin and leucin doesn't have a ring structure so chymotrypsin is only active on aromatique amino acids: tyr, phe and trp

[@DancinSurfer777]

You are correct that M and L do not have ring structures, however the specificity of Chymotrypsin is not the same as Trypsin, for example. In my Biochem book by Garrett and Grisham it says: "overtime, chymotrypsin also hydrolizes amid bonds involving amino acids OTHER than F, Y, or W. For instance, peptide bonds having Leu-donated carboxyls are also susceptible." It is similar for Met as well. Hope this helps :)

[@SLFOODtech]

great. Thank you very much

[@Kantsuhere]

Thank you

[@graceharris3650]

This was very helpful!

[@Remixam321]

super helpful video thank you soooo much

[@fahamidapuspa2569]

Can we also call this overlapping of protein sequence? Thanks for letting us know this!

[@wenderrodrigues1234]

Meu Deus, ele é perfeito!

[@nayabkhan5178]

This is amazing! Thank you so much :)

[@cakelytie]

methionine has no ring structure,so why does chymotrypsin cleave it?

[@gomezalejandrog]

because it cuts hydrophobic/bulky residues. Except Proline for some reason.

[@emanfathy888]

you saved my exam . tnankssss,,, I just have a problem with the board I can't see the words ,,, too smalll.... but thanks anyway...💓

[@ladanbigdeli8201]

I believe Chymotrypsin only cleaves at W, Y and F amino acids!

[@sarangas8688]

very useful sir

[@arkanshsharma850]

Going into the depth of each subject i mean at undergraduate level how many lives have you lived to gather that much information ?

[@Remixam321]

in the table under "cleavage site", is there a difference between carboxyle SIDE and carboxyl END?

[@nighthowlerx]

Thanks :D

[@youguesshuh3063]

Life saver

[@sudhasingh3798]

Sangers reagent??

[@juice28rsx]

great!!!!!

[@rahulbalaji3059]

The mathematical probability explanation

[@tomasturner9767]

Thanks for the great video :) I do not understand how the cleavage solves the problem of probability. Assuming I have a protein of 120 amino acids, and split them in 3 polypeptides of 40 amino acids. I have a probability of sequencing each correctly of 29,6%. The probability that all three are sequenced correctly I believe should be 0.296^3, which gives 2.6%. This is exactly the same as 0.97^120. Maybe I misunderstood something, if so please correct me, or maybe the accuracy of edman degradation decreases with the number of amino-acids, which would explain why we do the cleavage

[@arocks1234567]

the 0.296^3 isnt mathimatically sound you cant combine the 3 of them being done in 3 fragments as the same as them being done in the 120 aa chain. if you've done binomial dist you can connect the concepts its not the same as .97^120 because that's not how we did the Edman degradation

[@error_in_the_matrix4473]

thanks sir

[@jessrobson3029]

[@organic_angel]

MY PROFESOR LITTERALLY DID ONE EXAMPLE ON THIS IN LESS THAN A MINUTE WITH OUT EXPLAINING THE CONCENPT BECAUSE SHE SAID THIS IS NOT TAUGHT! THAT YOU CAN ONLY LEARN BY YOURSELF THROUGH PRACTICE AND SHE IS HAVING THIS ON THE EXAM AND I DONT UNDERSTAND AT ALL BECAUSE AGAIN SHE DIDN NOT EXPLAIN THE CONCEPT. LOL WHAT A JOKE, STUDENT LOANS GOING TO PROFESSORS WHO LITERALLY CAN'T OR REFUSE TO TEACH CONCEPTS, RATHER PAY YOU.

[@sabinahasanova7198]

Thank you