Diagonal Electrophoresis

[@kajalaggarwal5748]

Thanks sir...this video has very effective explanation!!

[@issaabujeries2912]

You're Awesome! Thank you!

[@chenellec3983]

Theoretically this makes a lot of sense... however, realistically you won't know exactly where your protein has been cleaved prior to running it on a diagonal PAGE. Wouldn't you have to purify and sequence the protein fragments following the diagonal PAGE to determine the approximate location of the disulfide bond?

[@Beadesigner023]

Thank you soooooooooo much

[@ahmedibrahim-mj2om]

Thank you

[@akhilb7649]

good one

[@junczhang]

AWESOME

[@paulturcott6816]

I was under the impression that with SDS-PAGE the proteins are trapped in the gel, making it useful for reference and characterization, but not for physical separation and further purification?

[@paulturcott6816]

+Paul Turcott It seems like gel filtration chromatography would be a better option for this? By the way, I love these videos man. I'm using them to brush up on basics before I go back to grad school ;)

[@doraexplorer4372]

THANKSSSS AWTSS GEGE

[@bioBeeLANT]

nojs