Analysis of Protein Purification

[@Cosmuex]

I owe this guy a lot of money for helping me get grades high enough to earn scholarships and jobs

[@tenipham6665]

just a small donation is enough

[@queena7672]

and college owes us money forgiving us failed professors

[@roshansaba1977]

Wat kind of jobs sir.. M also looking fa one

[@elibenaron]

Very good explanation, as usual. Thanks for being free.

[@joshuatanner5891]

Finally someone puts all these concepts together! Thank you!

[@tanushreelatiyan6675]

Thank you.. you explained each and every concept related to enzyme purification..

[@KJKP]

Basically, if you watch this video more than once, the purification level of your understanding will essentially increase.

[@junczhang]

how can anyone actually dislike your videos!!!

[@premaagarwal3387]

thank you so much. u made my concept crystal clear.

[@martinsafarian4404]

How do you measure “total protein” and “enzyme activity”?

[@OksalinaAls]

how did u calculate the enzyme activity in step A ?

[@apiin4611]

So, the final yield and purification of this mixture is depended on the step "E"?

[@cherissadover912]

How would we be able to calculate relative concentration?

[@jgramacy1]

why does enzyme activity decrease as we increase the purification? wouldn't it at least stay the same?

[@rehabalsaleh166]

It will usually decrease during purification, because protein is lost in various steps, and protein is also denatured during manipulations. Actually, this wasn't my answer. I'v just googled it and wanted to share it with you.

[@ThemisGeorgoudis]

great work man! cheers!

[@jasonophee6153]

this video triggered my ASMR

[@kareemjeiroudi1964]

Perfect! Connot be better

[@janberksevim8481]

thanks for sharing.

[@lakindublin]

Where's the continuation of this video?

[@yahyajaber3401]

thank you 🌹

[@mohammedelbehery1655]

thanks so much, but if the purified protein does not have enzyme activity how could you calculate purification level and yield

[@entertainment4you25]

How

[@bessekhalidatunnisa5931]

how to calculate total protein?

[@Ronkerboy]

Thank you so much

[@Brickkzz]

Speed: 1.25x... much better

[@ShakespeareCafe]

brilliant, but I kicked it up to 2.0x

[@kimbokjoo6817]

👍👍😂

[@ZaheerAbbas-su6fr]

can you provide a video about how to extract the enzymes from natural sources from both plants and animals sources

[@nikitagone9038]

Thankuuuuuuuu soooooo much Sir

[@Abeliever000]

Thank you soo much :)

[@Procrastinerd]

Is purification level a quantity that only applies to enzymes?

[@arlsffa]

+Cognitive Philosophy To anything that you are measuring or studying I think.

[@ibrahimibnal-haytham5208]

Opoops, I thought the dislike button was a Download button. Ma bad

[@xBeeeefy]

saaaaeeeeeempleeeeee