Bacterial Transformation

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This video demonstrates how to perform a bacterial transformation using the Bio-Rad pGLO™ bacterial transformation kit. The CaCl2 method is used to transform E. coli HB101 bacteria. Proper aseptic technique, heat-shock method, and sample labeling are shown.
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[@Nuttynatttt]

we have to do labs like this because of covid

[@VitorPereira-xy6bx]

ik :/

[@michaela9499]

girl same!

[@gxttersnipe1482]

low key upset i can't be doing a real lab

[@zaworudo3918]

lol and you can't even ask the professor questions. Just got to wait for them to respond to your messages a week late.

[@micahzile9430]

big sad

[@uberawsome3696]

As an amateur this is very useful

[@zaworudo3918]

Man I'm going to fail this class.

[@ElChavFam]

Same

[@vishnupullela3146]

😂

[@guacardo491]

maybe me too

[@Pablo-on7xm]

Just did it today it was easy, I'm in 7th grade💀

[@charan.ravuri7624]

Same situation

[@croberto7]

UofT BIO230 gang

[@Nicole72591]

still loving the coloring book!

[@reiani8283]

why does he low key sound like ethan from h3h3 lmao

[@DBS608]

Is this procedure works with this strain and/or the same plasmid only? Or this procedure can also works woth other strains and/or other plasmid too?

[@mattm345]

I’m a student worker and I’m trying to isolate the plasmid itself and we can’t seem to do it, I’m trying to find examples on line to see what part of it were messing up

[@cutepapaya4886]

Clarkson Bio II Lab Gang

[@proy7181]

Heat shock treatment right..

[@vardhanbivalkar822]

Does this plasmid give the bacteria the green fluorescent gene making them glow?

[@connorribera4198]

Yes

[@connorribera4198]

It contains the protein and the antibiotic resistance

[@kellyijang3542]

I hope it was just a display error and not a lab error at 03:31 when they said "transfer 250 microliters" but showed 520 microliters.

[@DBS608]

Its 250 ul only.

[@praveenrathore315]

Nice

[@cleo7663]

does anyone know why the petri dish should be placed upside down in the last step?

[@mriaz7289]

probably to avoid condensation from dripping onto the bacteria?

[@cleo7663]

@@mriaz7289 oh that makes so much sense. Thank you!

[@mriaz7289]

@@cleo7663 welcome!

[@rubinaaltaf999]

Great video!

[@jeremyprzygoda115]

Shoutout to everybody in Mr. Croteau's DNA Science class watching this

[@thekyloution3515]

Harrison Riggs was here

[@brookeholmes9223]

His glove is too big

[@davidfrancis2139]

Who is here from Philip's assignment?? RRC!!!!

[@icabero]

Is the process the same for yeast?

[@icabero]

Besides different growth media

[@نونه-ن3ف]

I don't understand English. Im speake Arabic

[@Born2beasavior17]

No results!!

[@mbangisenimuelelwa5880]

Wat is Lb stand for in plate

[@seabasszamarripa8252]

Luria Broth. It’s a type of nutrient that helps the bacteria grow!

[@_mustafaS]

NCSU gang

[@kaelinsherman7232]

Mrs. Zee bio gang

[@نونه-ن3ف]

lm speak Arabic