Yeast artificial chromosome (YAC) | What are the components of yeast artificial chromosome?

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Very good video. You don't miss a point to explain. Like also the positive and negative sites. Really helped me with exam preparation in gene technology. Go on like that :) really cool

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this video helped me more in my research, really thank you so much Dr.

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thank you so much for your videos🥳. I have a phD in Biohemistry and I have been watching all your videos to study for a very important test that I will have to take! Portuguese is my first language, btw😅 . Still, science is still universal! Your explanations are very clear and straight up to the point. Congratulations. Please keep recording!🥰

[@animatedbiologywitharpan]

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@@animatedbiologywitharpan sure thing, I will definetly share with friends who are studying with me for the same test. I think the problem in Brazil might be the language barrier between portuguese and english (Brazilians prefer videos in portuguese because english is not spoken here). That's why your ilustrations and worflow are so important , and you do them very nicely. Please don't stop recording, even if not so many people are showing up in the numbers. Your explanations have been really valuable to me and to everyone who seeks knowledge. You have all my respect and admiration. Thanks a lot, Arpan.

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That’s a great job, and it would be greater if you add subtitles.

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Thanks for the feedback...... I would take care about it in future

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Life saver ❤ .......I have exam at 11am

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[@nidhinaren]

Won't recombinant colonies be white? Sup4 is inactivated, hence pink colour won't be seen

[@dinaal-ezzi9441]

Thank you. For the insertion of gene of interest in yeast, we can use homologous recombination or only multiple cloning sites?

[@animatedbiologywitharpan]

through multiple cloning site only but checkouy my video on gateway cloning which use the principle of recombination

[@jimayla7446]

Very helpful sir. I have a presentation this morning on vectors

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Also my today

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Always happy to help

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Ur work really helpful for me. So thank you

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[@nouhailalamrabti9543]

when we are talking about yeast , its eucaryotic , so why we have a circular DNA , it must be linear no?

[@arthurc7365]

It is Linear after the space between the bam H falls off

[@fatemah-lc8od]

very good explanation , thank you for your videos

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Excellent explanation

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thank you sir, this section was very informative and easy to understand .

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Glad to hear that... I try to keep it short as I don’t wanna bore anyone...

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I am not any sir ..... just Arpan is fine

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[@mukarambhat]

As per Gene Cloning & DNA analysis by T. A brown 6E(P111), the presence of the insert DNA in the vector can be checked by testing for insertional inactivation of SUP4, which is carried out by simple color test, white colonies are recombinants & RED are not recombinants. While as per Analysis of Genes & Genomes by Richard J Reece: YAC in chapter Artificial chromosomes RED colonies are recombinants. Now what's correct

[@titikhyanath8361]

Red are recombinants. Insertional inactivation of SUP4 gene prevents suppression of ADE1 and ADE2 gene mutations. So now they will get mutated. Adenine synthesis will get interrupted and a red intermediate accumulates.

[@ammuponnu9601]

Thanks sir it very help ful and nice visualization ✨️

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@@animatedbiologywitharpan ok sir ✨️

[@magdalenaarendt5680]

Why do we have to digest vector with BamHI to make it linear? Can't it stay circular?

[@Navya20046]

Did you found the answer of this question?

[@magdalenaarendt5680]

​@@Navya20046 I guess it's because linear vector resembles Eukaryotic chromosomes which are linear and have telomeres at the ends. Maby if we wanted to use it in mice it would have to resemble mice chromosomes to work properly.

[@Navya20046]

@@magdalenaarendt5680 but if we want to use it in prokaryotes then... will we leave it as it is means circular?

[@magdalenaarendt5680]

​@@Navya20046 Yes I think so. I found intormation thet a circular YAC vector is able to replicate in E. coli due to the presence of bacterial ori and bla gene and is able to propagate in yeast as a linear molecule containing all necessery chromosomal elements: CEN4, ARS1 and two TEL which are functional in yeast after linearization.

[@Navya20046]

@@magdalenaarendt5680 OKkk thanks 🙂🙂🙂

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Excellent video i had ever seen

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such an out standing explanations

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@@animatedbiologywitharpan yes ofcourse sir. Please uplode more videos

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Very nicely explained

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[@muskansinghal4785]

Why centromere and telomeric sequence is important in YAC ??

[@mariamkhatib2379]

Thank you Dr But is there a video explaining the principle of selection of recombinant clones specifically in pYac 2 vector?

[@animatedbiologywitharpan]

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[@fenerbahcefan7902]

2:37 In real life this insertion (ligation) part never works. Instead you spend days to weeks troubleshooting. That's why I moved on to more efficient but slightly more expensive cloning methods like NE HiFi and Gap Repair. Of course, science being science those don't always work either. After 5 years in the lab and a worthless masters degree, I decided to go to medical school. Science wasn't worth it for me. I would've lost my mind if I had become a PhD student.

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I agree with you. Things are much difficult in reality than in pen and paper

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Great video sirji

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[@gautammoon9801]

Can you make these videos a little slower and also add a screen when you tell advantages and disadvantages like hearing those details quickly is difficult,if they are written it would be very helpful, And Amazing effort far better than any other explanation videos

[@animatedbiologywitharpan]

Thanks for the feedback.....it would be kept in mind for future....for now if you think the speed is fast you can slow the speed of video from settings

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Sir could you please add the English subtittle also

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Where is the link sir for further study about bac

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Basavesh BN due to low sound quality it was removed but would be uploaded soon

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Basavesh BN you can study about back from old primrose book

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Cool. Thanks!

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[@Rafi-tv5gc]

thanks for understanding yac

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[@mr.techfreak7436]

You have a mistake in this video Recombinant colonies are white in colour and u have mentioned that they are red in colour.

[@krupalisolanki7546]

Red colonies are non recombinants

[@wanhiskandar6270]

Mean?

[@aslu4921]

​@@wanhiskandar6270idk wt she meant here maybe insertional inactivation

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SHORT BUT AWESOME

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Sure dada

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After staying abroad I have some accent.... sorry if it bothered you... but it’s not something that I am trying to fake in order to portray myself as a cool guy..

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