Filtration and centrifugation of plant or microbial extracts//Whatmann filter paper//Centrifuge

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Dr. Raki's Just Biology

Dr. Raki's Just Biology

Күн бұрын

Пікірлер: 54
@abhijeetdarade7819
@abhijeetdarade7819 3 жыл бұрын
It's a commercial knowledge,and u r giving with free of cost Great person with great explanation Clearly audible understandable easily to digest
@Dr.Rakesh.B
@Dr.Rakesh.B 3 жыл бұрын
Thank you means a lot :) Just these small words keeps me going 🙏
@roshan71920
@roshan71920 Жыл бұрын
Well explained, currently I have started working on some plant extracts ,It was really very helpful.
@PremKumar-jz1ud
@PremKumar-jz1ud 8 ай бұрын
thank you so much anna
@yeroosanfiroomsa6321
@yeroosanfiroomsa6321 2 жыл бұрын
bro thank you .. really i like the way u explainl
@diegobianchi909
@diegobianchi909 Жыл бұрын
Hi Dr. Raki. I work with beneficial fungi of plants, generally considered safe for humans. I am interested in characterising the extracellular extracts with ethyl acetate. How would you suggest to operate to obtain the culture filtrate? Can I work on the bench Or do I have to do this in the safety laminar? To separate the broth, do I have to use the filter sterilisation with syringes (to remove the spores as well), or can I just filtrate with a Whatmann filter paper and think about getting rid just of the mycelia? THANK YOU
@Dr.Rakesh.B
@Dr.Rakesh.B Жыл бұрын
Its always advisable to work in laminar though ur sample is safe you can avoid contaminants from outside if your working in LAF. Filtering the spores would give you more exact information about crystals since your going for characterization spores might be a hindrance
@muhammadafan5054
@muhammadafan5054 Ай бұрын
For centrifugation we use rpm or rcf?
@sabasharif8969
@sabasharif8969 4 ай бұрын
Sir I have prepared extract so which size of filter paper I will use. There are many sizes so which size you will prefer??
@maqboolahmad9301
@maqboolahmad9301 Ай бұрын
Can you explain the process precipitation and production of auxin(synthesis) collection of auxin
@aartiuprikar5343
@aartiuprikar5343 2 жыл бұрын
sir I prepared different solvent extract silver nanoparticles... sir please suggest me that how the uv spectra performed of different solvent extract and I need the silver nanoparticle extract solidify or direct liquid solution is used for uv and which solvent used as a blank
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
Mail me rakeshb96@yahoo.com I'll send my silver nanoparticles paper
@mansirohilkar
@mansirohilkar Жыл бұрын
Can u please tell me .. that whatman paper can we use from both side ?
@Dr.Rakesh.B
@Dr.Rakesh.B Жыл бұрын
Yes
@user786sss
@user786sss 2 жыл бұрын
It is compulsory, Mentha extract have to stay 48 hours in room temperature ???
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
Mentha or methanol? What 48 hours ??
@user786sss
@user786sss 2 жыл бұрын
Mentha(Pudina/Mint) extract with solvent methanol.
@user786sss
@user786sss 2 жыл бұрын
It keeping 48 hours in room temperature , is that my question.
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
Yes it will evaporate all.the solvent then you can add known amount and redissolve. It's must if u do. For lower work at UG and PG we don't do so properly.
@sandart8070
@sandart8070 2 жыл бұрын
My doubt is the I use the methanolic solvent to the extraction of plant material but after extraction how to separate the ethanol and my plant extract when I used the your technique that is not sufficient because in the extract ethanol also be present so how I separate
@lilinito9510
@lilinito9510 2 жыл бұрын
The pellet is not horizontal with the tube Is it due to the angle of rotor ?
@kukankitasingh295
@kukankitasingh295 9 ай бұрын
Hi, sir currently i m working on the green synthesis of silver nanoparticles from plant extract can you help me please i will be very thankful to you .🙂🙂
@binitayadav8165
@binitayadav8165 8 ай бұрын
How to extract Bisphenol A (phytoremediated) from Plant?
@aabsheensaba6532
@aabsheensaba6532 3 жыл бұрын
I want to know how to prepare nanoparticle from plant extract?
@hakchangphananalanni7000
@hakchangphananalanni7000 3 жыл бұрын
Sir.. I have prepared plant extract using methanol and ethanol solvent.. Now I got semi liquid thick viscous extract... So, what diluent should I used to dilute this crude extract since it is not soluble in water.. Some said DMSO is best diluent but it is toxic.. Sir please suggest me what diluent should I used? Thank you sir in advance😊
@Dr.Rakesh.B
@Dr.Rakesh.B 3 жыл бұрын
You should use the same solvent , methanol and ethanol only. Since u used these solvents only in the first
@tilakdhoble2231
@tilakdhoble2231 27 күн бұрын
Thank You so much !!
@Dr.Rakesh.B
@Dr.Rakesh.B 25 күн бұрын
You're welcome! Happy to help.
@ruksanachowdhury1337
@ruksanachowdhury1337 2 жыл бұрын
Can i centrifuge volatile oil to separate residues
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
Yes you can centrifuge them at around 10,000 rpm for 10 min and separate the residues and collect the oil
@easychemistryofficial
@easychemistryofficial 2 жыл бұрын
Bro I made extract from seeds and it is an oil extract now how can I make its dilute solutions as in ur previous videos I have seen that if we have 1mg extract then we add 1ml solvent then what about oil?
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
You can add organic solvents which are soluble to your oils like chloroform acetone. Check the solubility practically or by reading papers
@easychemistryofficial
@easychemistryofficial 2 жыл бұрын
@@Dr.Rakesh.B Sir I want to ask about calculations... how much solvent should be add...
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
1:10 ratio for extraction. After evaporation add accordingly to make a known concentration. Watch the video on methanolic extract of plants for more info
@easychemistryofficial
@easychemistryofficial 2 жыл бұрын
@@Dr.Rakesh.B ok thanks
@aabsheensaba6532
@aabsheensaba6532 3 жыл бұрын
Very helpful
@AzizKhan-yt4xc
@AzizKhan-yt4xc 3 жыл бұрын
If we dont have rotory evaporator. What will be the alternative.
@Dr.Rakesh.B
@Dr.Rakesh.B 3 жыл бұрын
You can use hot air oven and dry it but the solvent will be evaporated so it can't be re-used
@lilinito9510
@lilinito9510 2 жыл бұрын
Thank you so much
@priyayadav2051
@priyayadav2051 3 жыл бұрын
Plz sir make a vdo on how to prepare sample for gc ms analysis...
@Dr.Rakesh.B
@Dr.Rakesh.B 3 жыл бұрын
It is already there in the channel priya. You can follow the same method how we made methanolic extract. Same method to be followed.
@priyayadav2051
@priyayadav2051 3 жыл бұрын
@@Dr.Rakesh.B ok sir...thanks a lot
@bibijarina9404
@bibijarina9404 2 жыл бұрын
Please do vedio for ftir sample for both extract
@kelechiaguguo9138
@kelechiaguguo9138 2 жыл бұрын
Pls. How do I calculate concentration?
@Dr.Rakesh.B
@Dr.Rakesh.B 2 жыл бұрын
What the video in my channel for methanolic extract preparation follow that for calculation
@rukaiyaalmin4334
@rukaiyaalmin4334 2 жыл бұрын
sir i need help to you how i contact to you?
@SNAVANEETHAKRISHNAN-k5y
@SNAVANEETHAKRISHNAN-k5y Жыл бұрын
procedure for the isolation of single compounds used in TLC Methods
@seemakedar3865
@seemakedar3865 3 жыл бұрын
👏👏👏👏👏
@kukkukukku9060
@kukkukukku9060 2 жыл бұрын
thank u
@sanidas6627
@sanidas6627 3 жыл бұрын
Thank you soo much Sir, I have an questions regarding antimicrobial screening. Can you share your email Sir
@Dr.Rakesh.B
@Dr.Rakesh.B 3 жыл бұрын
Rakeshb96@yahoo.com
@aneelazafar8495
@aneelazafar8495 Жыл бұрын
Very helpful
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