Bacterial characteristics - Gram staining | Cells | MCAT | Khan Academy

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[@mmaking8664]

OH MY GOD. Lady, you are truly a gift to us Microbiology students around the world. WHERE CAN I FIND MORE OF YOUR LECTURES? I WANT THEM.

[@oms7963]

@@drewduncan7702 stfu

[@drewduncan7702]

Only if you sub first

[@crystalleitch1130]

AMAZING!!!!!!! I sat in lab for 4 hours and l learned more in this 7.24 min clip. Thnak you

[@sophiaherleyy7382]

...I’m half way thru my semester and you just explained everything in a 7 minute video that I’ve been trying to understand the past 2 months. My professor SUCKS! Tysm

[@colekena5018]

OMG This is the best video I have seen thus far on Gram Positive and Gram Negative staining. Thank you so much for thoroughly explaining this process.

[@trinapooh1988]

My goodness lady! Before watching this video, I was completely oblivious to Gram-Staining, and how it works. I'm so glad God used you to put this video up, because he KNEW I would have microbiology spring 2016

[@XxAlex530xX]

Free Floating same here. They teach us like if we know everything

[@Luceium01]

@@XxAlex530xX fr. its fustrating

[@AAA97391]

This is gold.... thank you!

[@VibeMD]

Thanks a ton. Can you please make a video for the functioning of the ear and also for muscle fibers with all the muscle fiber's bands and the "gliding" method by which a muscle moves

[@reyianjohngayomali5178]

Thankyouuu, I understand it really well..

[@zoeyzenger5850]

I want to point out that while yes, the crystal violet does eventually wash off from the Gram negative bacteria, it's the iodine that encapsulates the Gram positive first, so they don't get decolorized by the alcohol. That's when the last stain (the Safranin) would stain the Gram negatives. I understand this is an educational video, and I know some of it may have been a little rushed for the sake of getting the point across, but I believe that understanding the process of staining and how it actually works is much more beneficial to viewers than a simple, "it stays purple because that's just what Gram positives do."

[@kosgengster1991]

Perfect! Thank you! Succint and the illustrations helped a lot.

[@pancakez227]

Great video. Te only grips I had was the "wash it off" part. As we know. You stain the bacteria with ultra violet stain, keep the stain on for 30 seconds, rinse with distilled H2O, gram stain for 60 seconds, rinse with H2O, tilt glass slide at 45 degree angle, then apply ethanol to the stain slowly(one drop at a time) then rinse with H2O, Safran stain for 60-120 seconds, rinse with H2O, then blot dry, then observe over microscope. The application of ethanol is very important in my opinion. But I digress because you can't think of all the details, such as I can't either.

[@nevaehchee1670]

thank you, you cleared a couple things I never quite grasped.

[@louisiannesaldagna5807]

i havent done my online biology in a month and this still makes sense to me. this is amazing

[@RahulPagar]

kzbin.info/www/bejne/fGm9nX1ujqilja8

[@thoufeeqahamed5489]

Well explained, thank you soo much! :)

[@irenenatsis9240]

AMAZING LECTURE THANKS

[@ramroom619]

Thank you to whoever contributed in making this video

[@ruisucepi]

THANK YOU!!!!!!!!!!!!!!!!!!!!!! I'm like WTH ! Why is it so easy to understand here compare in class lol! thank you, again for saving me once again. I went back to school after finishing LPN (I used Khan to study, too) and passing nclex and

[@hopejensen3929]

I completely understood this!!! Thank you 10000000 times!!!

[@prettyxpetite]

wow, this was great! but i have to keep watching it over and over.

[@Brynnerbrynner]

So well explained!! Thank You!

[@mcawesomeytyo3312]

Oh thank you, you are a gift from god. Your video saved my career

[@hannahdivic28]

Every time I see thumbs down for these videos I get really concerned. Idk if they got something wrong or if people just don't like it

[@mahadnur746]

Thank you so much! This was really helpful :D

[@Jojo-kl6tx]

Thank you so much. Great explanation, well structured (also the drawings) and on point.

[@astethoscope6468]

Thankyou so much !!!!

[@MultiPunk777]

nica and cool explanation. now i know why it was colored that way. hm.

[@RahulPagar]

kzbin.info/www/bejne/fGm9nX1ujqilja8

[@arunmoses2197]

Excellent explanation, thanks!

[@nsuhoreidemmichael1211]

This is like 2 hours of lecture and yelling from my lecturer..do you understand class..! picture they say is worth a thousand words..thanks for d video..did a whole lot of good in my understanding..but u didn't tell the arrangements of the bacteria..single(one) doubles(two's) in chains(long or short)..in clusters(ie bunches)..anyway thanks a bunch..!

[@aviaxferg]

Thank you!

[@kvcrao7472]

MADEM - BEAUTIFUL EXPLANATION - K V C RAO

[@mohammadal-ghanim825]

from iraq i very happy thenks

[@lukob6368]

that lady is excellent at what she does

[@jennyenobay444]

me be watching this day before microbiology exam

[@23vamsib92]

Nice explanation really helpful

[@talhakhan3989]

Plz explain the staining topic for class 11 student

[@maham5856]

OMG,,,its so helpful it help me alot

[@talhakhan3989]

Would you like to explain the circulation chapter briefly

[@shahadab1693]

Really helpful, thank you ❣️

[@RahulPagar]

kzbin.info/www/bejne/fGm9nX1ujqilja8

[@NicolasGanea]

Perfectly explained

[@charlieakz4237]

Helped a lott

[@XxAlex530xX]

Why can't my teacher be this simplistic

[@ChompNom]

im really impressed by the explanation, the actual purple staining splatter on the drawings. This is next level

[@Ara2061]

totally useful😊😊😊

[@rafiknabil2144]

You are amazing Khan academy

[@jjayrockz123]

Thank you so much

[@pharmacyera3421]

thanks much.

[@jeannie7919]

what part of the bacteria structure holds the stain?

[@addisonjune]

pretty sure its the peptidoglycan. which is why the negative doesn't hold it well, bc its peptidoglycan is so thin

[@jeannie7919]

@@addisonjune thank you

[@dr.monikabhuriya10]

Thank you medam.

[@Itsahilali]

i think she might be the reason i pass my exam in 8 hrs

[@rkm7174]

Thank you 🌸

[@sevenways2029]

Spirilla is not the plural of spirochete. Spirilla is the plural of spirillum. Spirilla and spirochetes are 2 different shapes. Spirilla are a loose coil while spirochetes are a tight coil.

[@amjadilham4174]

What does the teichoic acid do?

[@md.lutfarrahmanroni2060]

so nice

[@asmajahan6079]

Best lecture... thax

[@khaledali2414]

WHAT SOFTWARE ARE YOU USING

[@houstoncambodia]

Awesome video, though I feel like I should point out that spirochete and spirilla are actually different types of bacteria.

[@jollyjoseph8596]

Love youuu

[@roselitodaynatatoy8387]

nice

[@onyiimale]

Please where are your other lectures?

[@destinybasedoll]

how long does it take to perform and analyze a gram stain ?

[@marksmadhousemetaphysicalm2938]

If you're a klutz in the lab like me, longer than you might like...if you're competent...a couple minutes...

[@ShaneshaS]

If you need a fast method you could always try with a KOH test

[@tropicalstormxox9444]

what properties does the wall have that makes the dye stay on?? does a thicker petidoglycan wall absorb the crystal violet more or something???

[@StudyWithM3E46]

Tropical StormXoX yes. The thicker peptidoglycan layer holds the dye from the crystal violet. That's what gives it the blue stain

[@rosarose9642]

The thicker the peptidoglycan layer the more dye it will absorb therefore making it gram-positive. The thinner the peptidoglycan layer the less It will absorb therefore making a gram-negative

[@KyongchaeYi]

damn I should watch this video when quarter began danmmmmmmmmm

[@AmadaMia17]

Such an soothing and un intimidating voice.

[@Shirkietheshark]

Thank you! :)

[@jiahali5455]

The videos are really good but I think if you are teaching to medicos you need to go a bit more into the technical terms... it is too superficial

[@mariedoricecollet7894]

Spirochete plural version spirilla????

[@backstreetfan2887]

That is an error. Spirochete and spirilla do not mean the same thing. If you haven't yet, google differences between spirochete and spirilla and also check out the wikipedia page Spiral bacteria.

[@yasminejb8073]

thanks aloooot

[@user-rc8mb3zf7y]

Example of gram negative bacteria? ?

[@A11L9E]

E.coli, Enterobacter

[@IsabellKay9965]

omg this ladies voice is so sooo fucking cute 😍😍

[@saadrehman362]

??? her voice is so normal lmao

[@icysamurai1485]

Trick to remember that gram negative has a smaller peptidoglycan layer and a lipopolysaccharide layer: For athletic performance, it's negative eat more fat and carbs (lipopolysaccharide) instead of getting enough protein (smaller peptidoglycan layer)

[@redgeKagaoan]

2nd

[@micahpaik5445]

where my boy sal at tho...

[@ThyThy-ly3pi]

pp

[@kodywalter8348]

This lecture is not 100% accurate sadly. A little vague when it comes to the staining techniques, to say the least. Good color differentiation in the diagram along with the black background was very enjoyable however.

[@linkh1435]

Sorry for this late comment, but can you explain the inaccurate parts for me? Thank you.

[@jeffbaldwin3317]

@@linkh1435 I would add the function of the decolorizer: how it dissolves the outer membrane of Gram negative cells to facilitate counterstaining. Otherwise great video!

[@clintonbortz1537]

Thank you

[@17ReeM17]

thank u very very very much

[@safahmz5561]

thank you sooo much