Bacterial Smear Preparation

[@ericagovea9203]

Why aren’t you using any gloves ? Is it PPE not required ?

[@deerduster]

Na

[@kenlordnavarette8734]

What do you think would be easier to prepare bacterial smear from, liquid or solid culture?

[@abrarbhatti8978]

Send your whattsapp number

[@mood.my202]

هل وجدت الجواب؟

[@L4v4molly]

It's easier to obtain colonies separately from solid media. It's also more likely to carry unnecessary parts from the liquid media.

[@user-wk2gp3tu8h]

Thank you ❤️😊💯

[@ayatosakamaki92]

I'm confused , is the first drop of water compulsory???

[@Cleome-h4t]

Water is required only if its a solid culture from a plate but not for broth cultures

[@freefirebluezone]

​@@Cleome-h4tthnku

[@edidiongekpo]

Great video!

[@DatChoklateGurl69]

why didn't you have on gloves?

[@iradukundasylvie1148]

I guess it's not necessary

[@cassiloca]

you don't need them

[@kvnkvn9191]

Not necessary because culture is created so no contamination chances in sile by hand

[@ramkay9706]

youre dealing with fire, glove is not necessary

[@fluffandstuff6089]

Could cause a burn if the glove melts on your hand

[@althehore20]

Good ❤

[@yeppy013]

What is the purpose of air drying before heat fixing? Can't the heat fix do that as well as to dry the slide quicker?

[@angelaasencion397]

The water will boil out and the bacteria will be destroyed.

[@eclip9]

Heat damage them so you need to be careful already you can dry it on the flame but with keeping a good distamce in order to prevent it from getting hot

[@isiomawizzy3669]

Air drying preserves the morphology of the cell but heating distorts it.

[@MohammedAyaanQureshi]

Thankyou. Doc

[@maryamadarbeh2323]

Why it’s important to air dry the smear before heat fixing?

[@tayelorg8605]

Hi, I'm a microbiology student in my second year. If the sample isn't dry first you are likely to boil the bacteria instantly and all your cells may lyse destroying them.

[@LifeWithLonwabo]

why do we heat the mouth of the tube

[@wetherly]

Creates a hot convection current sterilizing the air and pushing out any unwanted microbes. Gotta keep the tube sterile at all times too for purity sake

[@beomnyang]

good job susan

[@sicelogemane9249]

Why the alcohol is used to clean the slide and not water?

[@SaraAli-cl8qs]

Perfect

[@epic11introvert77]

इस there bacteria in water and air will not affect it

[@محمدشاكرفليححسن.2]

good

[@lister2504]

Good video

[@zuluzero4659]

Where is ur lab coat where is your gloves?

[@CynicalDuty]

chase the bag, don't worry about what i'm doing

[@zuluzero4659]

@@CynicalDuty yes but your contaminating the results so thats wrong. Your also a threat to your health. I have got my youtube money hun x there is a protocol and h&s coshh for a reason if you work for any creditied lab or school and see you doing this, you will get reprimanded.

[@CynicalDuty]

@@zuluzero4659 bust down, thotiana

[@zuluzero4659]

@@CynicalDuty Uh, ayo, I been a bad b*tch, cockiana (Cockiana) / And I'm still winnin', Pacquiana (Pacquiana) / Percocets, popiana, killin' everybody beat / Thank you next, Ariana (Ariana)

[@tayelorg8605]

@@zuluzero4659 I'm not too sure which school you are referring to but at Curtin in Perth, in second-year Microbiology we don't use gloves. It is optional. As for the Lab coat yes it is standard PPE and we all need one. The results are not contaminated by bacteria on our hands nor can the bacteria on the stain infect us as it has been made bacteriostatic from use of the gram staining reagents. The bacteria on the slide are in too significant number to be contaminated by our own natural flora. Note also how they have been heat fixed first so our natural flora would just be washed off the slide if it was present.

[@noorjahan2246]

What she took from test tube??

[@2009abody]

Thank you

[@jesslynwong4666]

NO GLOVES ?! OH NO

[@senaityemanne422]

No gloves?

[@fluffandstuff6089]

Glove could melt and cause a 3rd degree burn

[@Oh-ou4lp]

ok where is the rest

[@asmajahan6079]

Thax dea

[@Hu_Ns1]

thik smear With out gloves 🤔

[@brunoasavaz8773]

「コンテンツを調整する必要があります」、

[@chandannishad4167]

Vedasi mam ki aaesi ki tasi . 😁😁👍

[@phali6253]

سلام الله على الله اذا افتهمت شي 😂🤦

[@طيبةيونساحمدحسن]

هههههه

[@y64]

ها ضبطتها؟ 😂

[@chandannishad4167]

Samaj Nahi aarha hai . English girl'. Jara ap Hindi me batiya . Ok 🍻😝😝😝