very wonderful explanation, thank you so much Sir!
@animatedbiologywitharpan2 жыл бұрын
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@Joel-fs5zhАй бұрын
this video is better than anything i've seen during my time at an american university, thank you
@animatedbiologywitharpanАй бұрын
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
@animatedbiologywitharpanАй бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@zacyo78516 күн бұрын
indian propoganda
@m.sivapriya16482 жыл бұрын
In this technique is based on structure analysis or functional analysis of recombinant bacteria
@pepsicolla1232 жыл бұрын
Thank you Sir, it's very informative
@animatedbiologywitharpan Жыл бұрын
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@aryanmusic92893 ай бұрын
after doing all the process u mentioned, what if the colonies doesnt grow, any troubleshoot can be done? please tell all the possibilities
@animatedbiologywitharpan3 ай бұрын
This page will give you a good idea about transformation trouble shooting www.thermofisher.com/in/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/bacterial-transformation-troubleshooting-guide.html?open=transformants#transformants
@nouraalq.6062 Жыл бұрын
amazing video
@animatedbiologywitharpan Жыл бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@David190508 Жыл бұрын
Thanks! I was wondering if I could get the powerpoint for this?
@animatedbiologywitharpan Жыл бұрын
Sure
@keepgoing66302 жыл бұрын
Great information, Thank you sir
@animatedbiologywitharpan2 жыл бұрын
Please share my channel link with your friends and juniors
@yuhengli6433 жыл бұрын
What treatments did not produce transformed bacteria?
@animatedbiologywitharpan3 жыл бұрын
If you just put the dna and bacteria in a tube and don't give headstock then transformation won't happen
@cristinaguanolemacoba77612 жыл бұрын
Best vid ever!!
@animatedbiologywitharpan2 жыл бұрын
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@shabnamnaderifar Жыл бұрын
thank you
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance
@johnparajo69163 жыл бұрын
Does only 1 plasmid enter the E.coli cell? Or do many of the same plasmid enter the cell?
@animatedbiologywitharpan3 жыл бұрын
Many many plasmids
@johnparajo69163 жыл бұрын
@@animatedbiologywitharpan If for example 8 plasmids are outside of the cell during incubation, do 8, 4, 3 etc plasmids go in? Or only 1?
@animatedbiologywitharpan3 жыл бұрын
Let's say 10 plasmids are there in the solution so ideally 10 should go inside ( assuming transformation efficiency is 100% ) . But in reality it's near 60-70% so 7 would make it and 3 would not . In reality we use plasmids in nanomol concentration. Now you can actually calculate the number of plasmid used for transformation.
@mightiestmouse88333 жыл бұрын
Great explanation!
@animatedbiologywitharpan3 жыл бұрын
Please share among friends and help me to reach big audience
@mightiestmouse88333 жыл бұрын
@@animatedbiologywitharpan I'll be sharing this video with other students in my biotechnology program!
@torlarsen22122 жыл бұрын
@@mightiestmouse8833 I also study biotechnology! High five 🙌
@keepgoing66302 жыл бұрын
Awsome explanation. would you like to share the Powerpoint?
@animatedbiologywitharpan2 жыл бұрын
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
@animatedbiologywitharpan2 жыл бұрын
I am so sory but the powerpoint is chargeable and not available for free
@animatedbiologywitharpan2 жыл бұрын
You can buy this powerpoint by paying me rs 100 via super thanks
@dhatchi7116 Жыл бұрын
Thank you 👍
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance . namaste 🙏
@artkitokri.....3809 Жыл бұрын
Sir please tell me, how to prepare plate with antibiotic?
@animatedbiologywitharpan Жыл бұрын
Prepare agar plates. Dissolve antibiotic in water. Mix into cooled agar. Pour, solidify. Seal. Store airtight. Maintain sterility.
@artkitokri.....3809 Жыл бұрын
@@animatedbiologywitharpan Thanks sir
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance. I am unable to reach many students. Please 🙏 help
@sj1h8294 жыл бұрын
What is the role of time in experiment? ex) 2min, 30min.... I want to know exactly......
@amandadiko23864 жыл бұрын
2:50 for 2 min 4:00 for 30 min The explanations are give at those time points
@ambseyyy2 жыл бұрын
thank you!
@animatedbiologywitharpan2 жыл бұрын
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@mustafanoor5123 жыл бұрын
thank you so much
@animatedbiologywitharpan3 жыл бұрын
please share my channel link with your friends and help me to reach big audience.
@ANKITKUMAR-fh9ze4 жыл бұрын
What is the role of putting bacteria initially on ice.
@animatedbiologywitharpan4 жыл бұрын
Just to give it a heat shock
@shoomayyy2 ай бұрын
I am saving my ass before exam 😂❤😂
@animatedbiologywitharpan2 ай бұрын
i am the ass saver. please share with friends and save their ass.
@GOLDEN_GAMING_3333 жыл бұрын
Thanks alot
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@jasminehasan70842 жыл бұрын
THANK U!!
@animatedbiologywitharpan2 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@muhammadalilvhd3702 Жыл бұрын
There was this mcq that confused me i.e the transformation efficiency increases if plasmid size increases upto ____ Bp
@animatedbiologywitharpan Жыл бұрын
What were the options?
@ahsanishaq87148 ай бұрын
UpTo 15kb ..after that efficiency decreases
@AdityaRaj-zk9eh11 ай бұрын
thanks sir
@animatedbiologywitharpan11 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@rabiagulsahin60533 жыл бұрын
Thanks
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@rabiagulsahin60533 жыл бұрын
@@animatedbiologywitharpan ı will :)
@ingkarissatayeva964 жыл бұрын
Thank you!!!!!!!!!!!1
@animatedbiologywitharpan4 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@priyanshasingh748 Жыл бұрын
this is exactly how i do in my lab
@animatedbiologywitharpan Жыл бұрын
😇
@animatedbiologywitharpan Жыл бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@priyanshasingh74811 ай бұрын
sure@@animatedbiologywitharpan
@kausalyaasarawanan18663 жыл бұрын
Thank you sir
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@1024DS3 жыл бұрын
what is the temperature at which bacteria can takes up the plasmid?
@animatedbiologywitharpan3 жыл бұрын
It's a heat shock ,it should be kept at 4C for 5 minute then 42C for 90sec and then 5 minutes in 4C .this shock would allow the entry
@jimmyramos62963 жыл бұрын
tested and proven ( TRANSFORMATION PATTERN ) Ingenuity Mags Process
@sachina55633 жыл бұрын
👍👍
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@samreetkaur260 Жыл бұрын
Sorry but drop the accent - it is irritating Your channel will grow
@animatedbiologywitharpan Жыл бұрын
Thanks for your suggestion, it's my inherent accent