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Baculovirus expression Vectors: AcMNPV

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Dr. PREM-PRIMER Biotech Lectures

Dr. PREM-PRIMER Biotech Lectures

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The specific baculovirus that has been used extensively is Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) • AcMNPV: Autographa cal...
The production of recombinant baculovirus expression vectors involves the following steps
1. A gene of interest is inserted into the MCS of Transfer vector and propagated in E. coli.
2. Next, insect cells in culture are cotransfected with AcMNPV DNA and the Transfer vector carrying the cloned gene.
3. Selection of recombinant AcMNPV genome virus particle

Пікірлер: 2
@noreenbashir3936
@noreenbashir3936 6 ай бұрын
Can we use the baculovirus expression system to express the avian viral proteins?
@Dr.DNA-Primer
@Dr.DNA-Primer 6 ай бұрын
Any gene can be expressed in baculovirus expression irrespective of the origin of gene. Try to subscribe channel to get regular updates.
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