Base Excision Repair | DNA Repair Mechanism

[@spicytuna08]

oh my God. i am from non-biology background. cannot believe our human has this jaw opening cellular dna mechanism. amazing how this was even discovered. thank you

[@hussainbiology]

glad to know that...

[@yazanabandah1677]

Ap Endonuclease cleaves the 5’ end .. and Lyase cleaves the 3’ end .. then DNA polymerase and DNA lygase play role. You forgot to mention the Lyase part! “Base-specific Glycosylase removes altered base and creates AP site (apurinic/apyrimidinic). One or more nucleotides are removed by AP-Endonuclease, which cleaves the 5′ end. Lyase cleaves the 3′ end. DNA Polymerase-β fills the gap and DNA Ligase seals it. Occurs throughout cell cycle.” GEL PLease! (The steps gylcosylase, endonuclease, lyase, polymerase, ligase) The quote is from FA USMLE step 1, biochemistry section (2 section), page 40.

[@Vinayak_Nair]

Thank you for this CLEAR explanation!

[@caterine4604]

THANKS HUSSAINNN !!! YOU ARE THE BEST !

[@hussainbiology]

Thanks Emila for appreciation...Glad to know that it helps.....

[@aussiejake4792]

excellent explantion! Thanks so much

[@ayeshahameed2859]

i just want you to know that all of your videos are awesome... keep it up... and help us by making more clear videos like that :)

[@hussainbiology]

Glad to know that my work helps you and i really appreciate your words ........It really boosts my confidence ..... Thanks Ayesha 😊

[@namratagupta207]

Thanks for precise and clear explanation 🙏

[@hussainbiology]

You are welcome ✌️

[@keerthichandranchoutapalli5153]

Your videos helps me a lot during my exams and makes easy to understand the topics short and very clear.. I really appreciates your hard work... Keep it up... Try to make videos in regarding to every concept in biology because some hard portions we are finding your videos... Once again thank you for your great work ✨

[@hussainbiology]

thanks for appreciating my work.... keep sharing

[@kannankamal5446]

Video excellent bro,but voice is not clarity,make sure for that

[@smahi4479]

Always been a big fan of your work.... Can you please do a video on Nucleotide excision repair?

[@sofiaahnen5076]

thank you! super helpful and clear!

[@user-nx1kk5gi2w]

T hank you

[@user-xz5un7vq4m]

Thank you for this through explanation!

[@beighmubashir4902]

Today I got to know that u r from Kashmir...I was thinking that u r Pakistani,,...I am from lolab valley kupwara and I am studying outside state and your vedios has helped me a lot from previous few years....Love u bro and now I am much proud on myself that whatever I achieved, some credit goes to u also ....Love u man 😊...proud of uhh

[@hussainbiology]

Great 👍 first of all thanks buddy for appreciation .... and where from u got to know i am from Kashmir ?

[@beighmubashir4902]

@@hussainbiology comment sections se pata chala bhai ...thanks to you I almost topped my batch after watching your vedios ..

[@harshavardhini6359]

Superb explanation

[@debbiehariette6335]

fantastic video, thank you :0)

[@hussainbiology]

you are welcome ✌️

[@pranalitupare3661]

Amazingly explained

[@hussainbiology]

Thanks Pranali for appreciation...Glad to know that it helps...

[@merve_n]

Thank you so much!!

[@mainakDXN]

Good one . Thanks

[@user-wh5df2cg2q]

very well done mate

[@ansari.zeba0748]

U r doing great...keep it up

[@hussainbiology]

Thank you so much 😀

[@temgodwin4033]

great explanation

[@Nishahappyhub]

Thanku sir🥰

[@hussainbiology]

my pleasure... Glad it helps ✌️

[@kushagarjuneja9996]

WONDERFUL!!

[@hussainbiology]

Thanks dear for appreciation

[@rainbowchicklol123]

This video is awesome!

[@hussainbiology]

Thanks Sarah for appreciation.....Keep supporting 😊

[@a45sankhadiptoghosh64]

Because of methylated cytosine converts to thymine by removing amino group and adding one h2o molecule

[@vinitaantil396]

Great work ... Keep it up...

[@yashusharma8781]

Plz recombination repair topic pr video bnado sir..🙏🙏🙏

[@hussainbiology]

Haan jaldi

[@crisprcas3537]

Thank u

[@hussainbiology]

Thanks buddy for appreciation....

[@a45sankhadiptoghosh64]

I learned that from your one previous video

[@BKmansha]

Nice nice very nice

[@hussainbiology]

Thanks Mansha for appreciation...

[@vishnuvish1647]

Vedio was helpful

[@srimanc.r9538]

replication, transcription and translation

[@hitkarshkushwaha2434]

good !

[@hussainbiology]

Thanks Buddy....appreciate ur support

[@Micro-life]

Sir wil u plz make a video on reversion mutation?...it is complicatd, evn im nt undrstng why reversions r used to tst mutagenicity.... plz sir make me clear?😊

[@hussainbiology]

I will definitely help you out.......

[@Micro-life]

Hussain Biology ...advance thnks sir😊😊😊

[@Micro-life]

Hussain Biology ...thnku sir fo sharing😊😊😊😊

[@hussainbiology]

My pleasure 😊😊😊😊

[@bibek8945]

You didn't clearly explain the work of Ap endonuclease. DNA glycosylase removed the base... And what did the Ap endonuclease do?

[@FOODIESWITHAQSA]

AP endonuclease cut phosphate sugar backbone and create a nick

[@bibek8945]

@@FOODIESWITHAQSA now i get it... Tq

[@JS-hh9zs]

One question though. How the DNA glycosylase know which base (in this case uracil), are the wrong uracil. After all, there are uracil also in immature mRNA. Why don't the glycosylase also cut the uracil from the right nucleotide? how it recognize the wrong uracil?

[@elahefa8433]

Do we have a short-patch and long-patch repair in prokaryots ? Is it kinda BER ?

[@hussainbiology]

Yeah….it is kinda a BER

[@MelomaniacEarth]

do you provide any notes in any ur website or in pdf format...it will be really helpful!!!

[@hussainbiology]

Apologies.. the website is not functional anymore and as of now I don't provide any notes

[@MelomaniacEarth]

@@hussainbiology its fine sir...the video itself was informative 🙂

[@navitahooda6427]

sir kya apki videos NET k liye b usefull hoti hain...?

[@hussainbiology]

Hi Navita.......Yes i try to make advanced level videos and I hope you will find them useful for CSIR net.... My Channel is totally based on CSIR NET.....

[@navitahooda6427]

Hussain Biology thanq sir

[@hussainbiology]

You are welcome.....

[@bijayachettri8689]

Sir you've deleted video on nucleotide excision repair ??? It was in your channel and i couldn't find it ..will you let me know you deleted or its there???🥺

[@hussainbiology]

No no.. i don't think i have made a video on that topic..... Ya toa mai he bhool gaya hoon

[@hemjoshi1724]

Sir u don't have nucleotide excision repair lecture video i need this

[@hussainbiology]

Apologies.... I haven't made a video on that yet but i will definitely consider your suggestion .

[@hemjoshi1724]

Hussain Biology sir is it possible tomorrow because I have exam ur previous lectures helped me in understanding the topic so I need for this also if possible

[@hussainbiology]

Sorry Hem.......It is not possible to make a video and edit it within few hours...

[@hemjoshi1724]

Hussain Biology ok sir

[@hussainbiology]

Apologies,,,,,Couldn't help

[@nabeela6467]

sorry what is AP Site?

[@hussainbiology]

apurinic or apyrimidinic site. Where Purine or Pyrimidine is missing.

[@ComandaKronikk]

@@hussainbiology I've been told by my professor that AP sites are strictly for missing purines

[@hussainbiology]

@@ComandaKronikk Actually apyrimidine site is very rare since it requires high acidity to cleave off the pyrimidine from the site where as Apurine site is easily formed in the cell at lower acidity or even under neutral pH. that is why your Professor has told u it is mostly Purines.

[@emad9703]

Where is nucleotide excision repair???

[@funkkern8512]

base excision what 😳

[@cozmonauts932]

Rapier

[@BIotechnologywithAk]

❤❤

[@Thewiseenigma]

Sr nucleotide repair ki video kb banaoge 2 sal ho gai

[@doc.3li]

👏🏻👏🏻👏🏻

[@basharatali927]

you are from kashmir? I can tell from your sound..lol😁

[@hussainbiology]

Indeed i am Kashmiri...

[@basharatali927]

which district? srinagar?

[@hussainbiology]

@@basharatali927 Baramulla Pattan

[@basharatali927]

ok sir BTW I am a sheena dard from Gurez valley bandipora ..

[@hussainbiology]

That is great......Means i have now subscriber from Gurez also...,😊😊

[@a45sankhadiptoghosh64]

I think the hydrolytic deamination results in cytosine to thymine not uracil

[@LinguisticLuminosity66]

It's not gounine it's guanine.... correct the pronunciation Mr. 🙂

[@hussainbiology]

Apologies....i accept i am bad at pronunciation but the point is... u understood still

[@honeykushwah7200]

Plz teaches in Hindi

[@wessel175]

your pronounciation is a bit of. You pronounce repair as rapier. Good pronounciation is like r eee p eehhhhh r.

[@karishmadeegan8297]

90 percent of his audience is Indian so I don't think pronunciation is a major concern for him. Yes it is slightly off but it doesn't need to be perfect. It's still understandable for most of his viewers :)

[@janhavikuvar173]

Tere se pucha??

[@Snaomib]

L take

[@kannankamal5446]

Video excellent bro,but voice is not clarity,make sure for that