Carbohydrate Testing | Testing Sucrose (Disaccharide)Benedict Test | Fehling Test|11th Grade Bio Lab

teste of sucrose
juice or sugarcane or sucrose solution
add a few drop of HCL and boil the test tube genitaly for 1 or 2 minute
this hydrolysis sucrose into glucose and fructose
make the solution alkaline with NaOH
now perform Benedict or falling test with this solution for the presence of glucose
Orange aur brick red PPT is observed in the test tube
positive test with benettic solution show the presence of sucrose in the juice or solution
#biologylab
Certainly, here's a more detailed procedure that includes the steps involving acid and base for breaking down sucrose into glucose and fructose before conducting the Benedict and Fehling tests:
*Aim:*
To test for the presence of sucrose (a disaccharide) by hydrolyzing it into glucose and fructose using acid, followed by testing with the Benedict and Fehling tests.
*Requirements:*
1. Sucrose solution
2. Hydrochloric acid (HCl)
3. Sodium hydroxide (NaOH)
4. Benedict reagent
5. Fehling solution A and B
6. Test tubes
7. Water bath or heat source
8. Bunsen burner (if required)
9. Pipettes or droppers
10. White tile or background surface
*Procedure:*
1. Prepare the sucrose solution: Obtain a solution of sucrose (disaccharide).
2. Hydrolysis with Acid:
a. Take a small portion of the sucrose solution in a test tube.
b. Add a few drops of hydrochloric acid (HCl) to the test tube.
c. Heat the test tube in a water bath or over a Bunsen burner for a few minutes to hydrolyze the sucrose into glucose and fructose.
d. Neutralize the solution by adding a small amount of sodium hydroxide (NaOH).
This will ensure that the solution is neither too acidic nor too basic.
3. Benedict Test:
a. Take a small portion of the hydrolyzed solution in a test tube.
b. Add an equal volume of Benedict reagent to the test tube.
c. Heat the test tube in a water bath for a few minutes. Observe any color change.
4. Fehling Test:
a. Take a small portion of the hydrolyzed solution in a test tube.
b. Add equal volumes of Fehling solutions A and B to the test tube.
c. Heat the test tube in a water bath. Note any color changes or precipitate formation.
*Results:*
Benedict Test:
Positive: Formation of a brick-red precipitate indicates the presence of reducing sugars (glucose and fructose).
Negative: No color change indicates the absence of reducing sugars (sucrose).
Fehling Test:
Positive: Formation of a reddish-brown precipitate indicates the presence of reducing sugars (glucose and fructose).
Negative: No color change indicates the absence of reducing sugars (sucrose).
*Trending Tags:*
1. #CarbohydrateTesting
2. #BioLabExperiment
3. #BenedictTest
4. #FehlingTest
5. #CarbAnalysis
6. #BiologyLab
7. #HighSchoolScience
8. #CarbDetection
9. #Biochemistry
10. #LabPractical
11. #BiologyEducation
12. #ScienceExperiment
13. #CarbIdentification
14. #BiologyClass
15. #BioLearning

[@GSB2760]

You do very well good job and make sure one day you get more importance 😊

[@LetMeExplainnowbharatpanwar]

Thanks for your love

[@aryarajsinhjadeja325]

It is really very helpful 👌👍

[@amita.s.chauhan2442]

thankyou sir aapka samjhane ka tareeka chamatkaari hai

[@BapiSk-o4o]

Bohot botoh thanks

[@Otaku_Saitama]

Tomorrow is my practical

[@lprlbiology5770]

Amazing sir

[@MrMNg-q6j]

What is the concentration of the solution?

[@monikachoudhary1516]

I am in bds and this is very helpful for me

[@LetMeExplainnowbharatpanwar]

Thank you dear. 😊

[@ZohaibNaich-di9ek]

Good sr❤❤❤

[@___-yb9fu]

Blue: No glucose Green: Trace amounts of glucose Yellow: Moderate glucose Orange: High glucose Brick red: Very high glucose

[@LetMeExplainnowbharatpanwar]

Absolutely right

[@rakeshod7447]

Aa pereticel no kayo che te kaaho sir please

[@sanjeev6185]

Sunder

[@prakashbhandari5824]

SIr in the description of procedure in benedict test results you have mentioned sucrose as a reducing. I am confident it is non reducing please rectify it

[@Anekhan1922]

Yes it is non reducing sugar.

[@LetMeExplainnowbharatpanwar]

Sucrose non reducing

[@dr.gurvinderpalsingh2146]

Sucrose is polysaccharide

[@LetMeExplainnowbharatpanwar]

No it's disaccharide