Confocal microscopy Basics

This video aims to describe how confocal microscope works and how it is different from fluorescence microscope

[@SaganIII]

I love the background sound “Boah”

[@animatedbiologywitharpan]

Ha ha ha....... that sounds funny

[@ASITV]

this sound improves my focus on confocal microscopy

[@georgesaide2953]

Very informative, and well said. Thank you!

[@harshoza1399]

Beautifully explained. Can you upload a video explaining Phase contrast microscopy? Please....

[@rydeen503]

This was great information. Thanks for taking time to explain how Confocal Microscopy works!

[@animatedbiologywitharpan]

Watch this one kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@animatedbiologywitharpan]

Please share among friends and help me to reach big audience

[@bsp127]

This was an excellent explanation of this technology. Well done. Thank you very much!

[@animatedbiologywitharpan]

Please rever to my another video for better explanation kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@animatedbiologywitharpan]

If like my explanation please share among friends and help me to reach big audience

[@JohnTaylor-nk8cp]

This is beautifully explained, thank you so much. Phenomenal video

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@Jindy2]

Excellent! Clear and concise. Many thanks.

[@animatedbiologywitharpan]

There is a second part to it .... watch my microscopy playlist

[@animatedbiologywitharpan]

Please share among your friends

[@saakshibhayana1532]

Very useful. Could easily understand for the very first time. Thanks a lot. :)

[@animatedbiologywitharpan]

thanks, more videos would be uploaded soon on confocal microscopy and image analysis

[@vladimirkesaev6818]

A good explanation for a quick first introduction. A minor inaccuracy on the energy level diagram. Thanks and continue, please.

[@animatedbiologywitharpan]

Thanks for your feedback and suggestions. Here is an updated version of the same video kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@sumitborhade3178]

The formula should be D= 1.22 lambda/ 2 X NA, right?

[@adamsmith467]

Very well explained

[@deepakkakara23]

Thank you, you made it easy to understand

[@animatedbiologywitharpan]

Please share my channel link in your college group

[@atefalmalky9268]

thank you

[@naomi5817]

Awesome explanation, thanks!

[@animatedbiologywitharpan]

Glad to know it’s helpful.... share among friends....

[@animatedbiologywitharpan]

kzbin.info/www/bejne/fHvFn5Kbjd90atE in case you want details

[@maiwennlilas9662]

Very good! thank you!!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@WillXing]

This is a great introduction of the principle of confocal microscope. However, there is a mistake from 10:00 when you calculate the pinhole diameter using point spread function: D= 1.22λ/NA - this is the size of the illumination point on your sample, not the physical pinhole diameter. For physical diameter of the pinhole, it need to be multiplied by magnification (of the objective), which give the optimal pinhole diameter D = M x (1.22λ/NA), where M is the magnification of the objective lens you use with your confocal (x60, x100,etc). It is worth to point this out in case mislead people who are studying or developing system related to confocal microscopy. Overall, great video, pretty clear and straightforward explanation. Thanks for sharing:)

[@animatedbiologywitharpan]

Thanks for giving a detailed feedback.

[@konradoization]

Thanks for pointing that out! - I was already wondering how such super-small pinholes with diameters of 500 nm would be realized mechanically.. Otherwise thanks for the great video arpan! :)

[@pacher72]

It helped a lot thank you so much

[@animatedbiologywitharpan]

There is another video in the playlist named confocal microscopy details......do watch that as well

[@animatedbiologywitharpan]

Might be helpful

[@hendahmad9510]

Well said Thanks

[@animatedbiologywitharpan]

Watch the second video about confocal for details

[@karcicegi5366]

Thank you so much

[@animatedbiologywitharpan]

There is an advance version of this

[@animatedbiologywitharpan]

Check my playlist...might be useful

[@abigailparry3264]

Literally a blessing

[@animatedbiologywitharpan]

Watch this part 2 of that video as well kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@animatedbiologywitharpan]

Share among friends and help them as well

[@MrNailbrain]

If an electron goes from S0 to S1 and then back shouldn't it emmit the same light as it absorbs? What happens to the 21nm worth of light/energy?

[@chantalusai3242]

There is an error there, there should be other vibrational states higher than S1 and the blue arrow should land there, hence the Energy difference. 😅

[@rockhardX]

Set for my presentation thank you

[@animatedbiologywitharpan]

Do watch another video on confocal from my microscopy playlist....it’s a detailed one...share among your friends and help me to reach big audience

[@animatedbiologywitharpan]

Feel free to get in touch if you need help

[@nabhasjyothis4373]

This helped me a lot..thnks

[@animatedbiologywitharpan]

There is a detailed video on same topic and I suggest you watch it

[@animatedbiologywitharpan]

Please share among your friends and help me to reach big audience

[@shailenderrajput3500]

U my friend is a treasure which must be save

[@animatedbiologywitharpan]

Ok I see

[@animatedbiologywitharpan]

i dont completely understand the comment is sarcastic or genuine. But anyway if you find the content useful please share among your friends and help me to reach big audience

[@dipayanbairagi616]

saved me from spending one hour on a book chapter.

[@user-nx7hs7kz3l]

agreed! >

[@nithyasahayarubi3666]

Thank you so much sir

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@nithyasahayarubi3666]

@@animatedbiologywitharpan ofcourse sir

[@jessimay987]

Does anybody know the max resolution and magnification for this type of microscope?

[@animatedbiologywitharpan]

Theoretically it can separate 2 points that are 200nm apart

[@animatedbiologywitharpan]

Practically with higher na objective it and if you use 488 lazer to illuminate do it would be roughly 300 nm

[@jessimay987]

Thank you!

[@beardog6569]

Dude has mad drawing skills

[@animatedbiologywitharpan]

Please share my channel link with your friends and juniors

[@amitkhatti6807]

Well explained

[@animatedbiologywitharpan]

Soon I would upload a detailed video on confocal

[@animatedbiologywitharpan]

kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@yiyiyao7165]

great explanation and very helpful! but the noises in the background are a little distracting

[@animatedbiologywitharpan]

kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@animatedbiologywitharpan]

This video is the second part and better one

[@animatedbiologywitharpan]

Don’t forget to share among friends and thanks for suggestions

[@lotharmayring6063]

a normal achromat is confocal if you use a pinhole

[@Bio-tec]

Reduce back ground disturbance.

[@user-hd7pn2is3k]

if both my professor and you drown in water i shall save you first

[@animatedbiologywitharpan]

Eugene Moon please share among your friends

[@animatedbiologywitharpan]

Eugene Moon but you should have watched the advanced version of this video not this one ....it too much basics .Please watch the following kzbin.info/www/bejne/fHvFn5Kbjd90atE

[@user-hd7pn2is3k]

@@animatedbiologywitharpan i needed to learn from the crude basics, but thank you anyways :) i will watch your recommendation too thanx!!

[@sakshisood7108]

Please use ur own accent..U already have a good one..Otherwise good video..Thanks

[@animatedbiologywitharpan]

sakshi sood I appreciate your feedback.... it would help me to improve in future

[@lg691]

"Microscopy"

[@lotharmayring6063]

confocal must not be fluorescence

[@abdalkaderali7176]

Not clear

[@animatedbiologywitharpan]

kzbin.info/www/bejne/fHvFn5Kbjd90atE Please watch this video...it would be clear

[@hitikapuri7388]

So irritating background noise.....aaahhhhhhhhhh

[@animatedbiologywitharpan]

There is another video which don't have this back ground noise called " confocal microscopy details "

[@hitikapuri7388]

Thanks... tomorrow is IIT jam exam....

[@animatedbiologywitharpan]

All the best

[@hitikapuri7388]

Thank u so much..