COPII Vesicle Formation

This animation shows the formation of a COPII vesicle and its transport to the Golgi apparatus.
Interesting talks about this topic:
Randy Schekman (Berkeley) Part 1: Studying Protein Secretion in Yeast (iBiology)
• Video
Randy Schekman (Berkeley) Part 2: Biochemical Reconstitution of Transport Vesicle Budding (iBiology)
• Video
Randy Schekman (Berkeley) Part 3: Human Diseases of Vesicle Budding (iBiology)
• Video
The Golgi Apparatus and ER to Golgi (Anterograde) Transport Part 1 - 4
• The Golgi and Anterogr...
Further readings:
COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. (Barlowe et. al., 1994)
COPII and COPI Traffic at the ER-Golgi Interface. (Szul and Szrul, 2011)
Assembly, organization, and function of the COPII coat. (Hughes and Stephens, 2007)
COPII and the regulation of protein sorting in mammals. (Zanetti et. al., 2012)
Music: The 126ers - Secret Conversations
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Ribosome Studio is an animation studio based in Switzerland. We produce high-quality scientific animations, medical animations, and mechanism of action movies. For more information go to www.ribosomestudio.com

[@ivanbykov7649]

That's the coolest and the most stylish animation I've seen so far in cell biology videos. Everything was super clear even though there were no verbal comments. Excellent work!

[@violinsheetmusicblog]

Nature is so beautiful!

[@Poetry_Maiden]

I actually shed a tear watching this :') thanks for the video, it's awesome to watch such beautiful animations while studying.

[@Ribosomestudio]

This animation shows the formation of a COPII vesicle and its transport from the ER to the Golgi apparatus.

[@jaimereyes6288]

Thank you, your video is helping me in my studies! :D

[@user-qx7wx2zo3g]

James Reyes so am I ! How clear the video is. Thank you very much

[@sab8249]

What a beautiful animation!! It is very useful to see proteins in a realistic formation to understand how these mechanisms truly work. Thank you so much!

[@baraskparas9559]

Fantabulous. A movie is worth a million words.

[@alessandrobroli5691]

Thank you so much this makes it so tangible !!! Thank you thank you thank you. Love thèse animations so much

[@hcsantiago]

Simply awesome. Thank you for doing this... with the music, it turned poetic.

[@millard4303]

My god,,! This animation made me cry,,,,so lively,,so real,,so touching,,,and to see how those molecules pulled up the membrane to make a vesicule ,,shocked me,,,how basic life knows that much engineering,,, also that transporter who simply walksssssss ,,my godddd,,i have usmle and im overwhelmed by these realities

[@huntermansfield9615]

This video changed my fucking life. Kevin Macleod's music brought the essence of life into my soul as I witnessed a simple protocol of life unravel into a great complexity. Thank you, my life makes sense now

[@cecehemmerle5123]

extremely well done, thank you for this visual

[@glukeye9278]

beautiful

[@ellamokrushin6458]

This is beautiful!

[@silverhairpin7569]

What an amazing animation. Thank youuuu

[@omrhp]

thank you very much! really helped me visualize :)

[@mohammedvalikarimwala4700]

Just next level! 💯💯

[@TommyZommy]

Saved me 3 hrs before my exam lol! Thannk you

[@patycarrascozuniga6331]

This animation is amazing!! ;')

[@elinenorin4242]

That was beautiful

[@shreyashah9598]

Nice animation!

[@sandiestsolid08]

no lie that was pretty lit

[@miiaaalvarez]

The BEST thank you!

[@jakepicker7363]

This animation feels like an early 2000s Sitcom intro

[@dilansor4838]

Beautiful

[@noranmohammed8879]

So exciting Thank u

[@rheaverma6295]

WOW, thx sir 4 da video

[@CherubCow]

That is so beautiful. You can't tell me that there's not a Flying Spaghetti Monster! Look at FSM's noodly appendages helping to form COPII vesicles!! Such complexity could only arise from spaghetti intervention!

[@ZombieNinjaTurtle]

Ramen!!!

[@crow33215]

Haha yes! It's more logical to say that this happened just because! Ramen xD

[@iancoats9946]

If vesicle formation was an anime this would be the intro

[@d.deepak]

good video, try to add voice notes or subtitles of what happening there in a cell at that particular time

[@TheToeProject]

Was this done in Blender? Incredible work! I would've added the text in your video editor afterwards, though.

[@jaquelinevanesalopez198]

Thankssss 🥰

[@NarekAbelyan]

COOL !!!

[@tovahwilliamson244]

the music is actually "Secret Conversations" by The 126ers!

[@Ribosomestudio]

You are right. Thank you for letting us know. We changed it in the description.

[@AlmeAedo]

I'm in love

[@manishalohan4005]

and all my doubts come to an end😁

[@tamannaalam2938]

motor proteins are BAE

[@SeagullBox]

high as hell and this video just made me believe in god

[@clarafrancobenitez3801]

Videazo

[@elijahw.h.1134]

what anime is this

[@Farmacia-hi1dl]

FORMIDÁVEL!

[@aphps]

😱😱😱😱

[@darrendsz9459]

Nice video to watch, but I have learned nothing from it.

[@violinsheetmusicblog]

SEC12 phosphorylates SAR1. This causes SAR1 to attach to the membrane of the ER. Then, dimer groups SEC23/SEC24, and SEC 13/31 aggregate around it. They cause it to curve and pinch a vesicle out. Then, SEC23 dephosphorylates SAR1 back to GDP, causing the entire complex to fall apart.

[@Grindelwald6]

thx

[@lyndonnewins1089]

Sec12 is a GEF (Guanine nucleotide exchange factor) not a kinase, it exchanges Sar1-bound GDP for GTP. Sar1.GTP can then bind the ER membrane by the shallow insertion of its N terminal amphipathic alpha helix. 23/24 and 13/31 don't aggregate, they bind each other to form COPII cages which shape the ER membrane to make vesicles. Sec23 is the GAP (GTPase activating protein) which accelerates the hydrolysis rate of Sar1, a GTPase (it doesn't dephosphorylate Sar1 since it residues of Sar1 were never phosphorylated). This converts Sar1.GTP to Sar1.GDP, the soluble/non membrane-associated form which is thought to trigger vesicle uncoating.

[@thetrollpatrol8799]

That is so beautiful. You can't tell me there's not a God. Nature is insanely complex

[@bluecheesybob]

Good one, troll.

[@janakakumara3836]

All of the structures you have seen in the video are created because they are encoded in DNA. We know that DNA mutates randomly resulting is small changes to these structures. If the differences help the organism survive and reproduce those changes are passed on. Otherwise the organism dies. In this way, over billions of years, the DNA has picked up the instruction set of things that encode for modern living organisms. The trace of of the changes in DNA has been observed in closely related species. It is facinating that our Universe can create such complex things via a process of mutation and creative destruction and refinement, but none of it was designed by an supernatural being. We know enough to know that it does not need to be designed.

[@Somenath_Sen]

Kindly do not insult the tireless work of the cell biologists who have given their whole lives in describing intracellular trafficking by saying things like these.

[@loudequalsfunny1496]

Uhm, just by seeing that video you can really tell that there's no god. No need for a creator. Cells have developed on their own. Thank evolution,not god

[@mateozapicogarcia6929]

​@@loudequalsfunny1496 Slow down, he was just commenting how awesome nature is, without insulting anyone.

[@reenamalhotra3164]

Nice but for a new student difficult to understand so give notes

[@filibertoaugustobautistamo9305]

Mi

[@fernando8661]

Tiene un error este video