CRISPR is genome vandalism | Manolis Kellis and Lex Fridman

Lex Fridman Podcast full episode: • Manolis Kellis: Biolog...
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[@tanxyrogue847]

Most clear explanation of CRISPR I've ever heard

[@bsmith1310b]

Yes!

[@markstewart4501]

Out of trying to make better f'ing yogurt. WOW.

[@ticket67]

This guy is a real professional. Would be great to have more of this on youtube.

[@charliecasson1643]

True, very well spoken and highly intelligent, his podcasts are some of my favourites

[@ticket67]

@@charliecasson1643 The most important thing is that he really knows what he is talking about. A professional.

[@melodydeese1964]

You should buy their stock if it turns out good, may make a fortune.

[@questionable-ethics]

Can anyone recommended some resources to learn more about cripsr and its methods of use

[@BradHolkesvig]

If you think of everything as information, then it's easier to understand. Information is used to change other information. You can also think of a pond of water that is completely still. Then by throwing a stone in that water, it changes the appearance of the water in that pond. This is information changing information.

[@100ghillie]

Good analogy

[@rifz42]

I wish the link to full episode was time stamped to the end of this, so I can carry on with this part.. great show! thanks!

[@markstewart4501]

What a great idea. Agreed.

[@theflyingcrud]

This is fantastic! Such a clear and concise explanation, I'm blown away. Thank you for this!

[@eugkra33]

this yogurt industry is going to be a trillion dollar industry one day when it starts to cure aging.

[@markstewart4501]

Imagine buying yogurt based on the labeling of a human aliment...in a grocery store, or even prescription based yogurt. Haven't read that in my sci-fi books.

[@markstewart4501]

@@concernedliberal4453 future, future, future studies sponsored by yogurt providers will publish their self found results...so...yeah, the fruit may or may not count. The study will be heavily dictated by public opinion. SUPRISINGLY!!! They will show that we need to abandone the butter vs. margarine studies. Yogurt will then work its studies to outwork the benefits of chocolate, coffee, some exotic berry from the south pacific, and what size of glass of wine a day...works better than apples. Capitalism, financing vulnerability for cash strapped science for decades now. aka...the new truth...ah statement is science now.

[@kipling1957]

It's refreshing to see my field be a revelation to Lex, e.g., CRISPR-CAS9 technology, instead of always me trying to keep up the computer/AI stuff.

[@MM-qw4eo]

The whole 2hrs or so was pretty cool too

[@filipg2834]

The one question regarding gene editing I always had is how does the DNA modification propagate through your entire body? Where do you need to make this gene change doesn’t every cell in your body contain your genome? Do you need to make the edit in every cell at once?

[@rizizum]

For affecting your whole body you would need to modify the DNA of most of your cells, or just change the DNA of the egg cell you would come from. To change the DNA of most of your cells as an adult you would have to do something called gene therapy, which is not perfect at the moment as far as I know

[@BroCactus]

This is precisely the reason gene therapy is not safe or effective in most instances. It is easy to transfect a single cell in vivo with CRISPR or another gene editing technique. But most genetic diseases affect billions of cells throughout the body. Correcting only several mutant cells would result in something called mosaicism. The techniques which are effectively able to transfect all or most cells, namely viral vectors, are among the most risky.

[@kenkclam]

There are currently no efficient ways to edit gene in all cells in an adult. One can edit the embryo so the edits will be carried by all cells, but that is a bad idea becuz the edit will go to your offspring and will be in human gene pool forever. I think the aim should be ex vivo gene editing, say for your blood cells.

[@dandeelyonn]

Editing cells while they are still embryos is possible, but editing the cells of adults is tremendously difficult. It would be possible to edit stem cells and then insert them so that they produce healthy cells with genetic modifications- but right now, the amount of knowledge and safety research required is too much for it to be realistically feasible.

[@hamooon]

@@kenkclam ex vivi means to take it out of the body, do it there? Like take a bloodcell, edit it and have it multiply, then put the result back in the body? (I'm a pleb)

[@tomlloyd9364]

Beam doesn’t cut the genome, it chemically changes one side and nicks the other side to change each letter. No more cutting needed

[@tomlloyd9364]

@@eliudnjai that’s not cutting. Cutting physically takes out dna and replaces it. Beam changes what’s in the dna already.

[@kenkclam]

Cutting can be a confusing word as some regard nicking as cutting of single strand DNA. Maybe easier to understand if we say BEAM does not use CAS9 to create double strand break. It uses base editor to modify one DNA strand and Nick the other strand.

[@tomlloyd9364]

@@kenkclam whoever regards nicking as cutting should just learn the proper definition instead of having to re-explain using more words. The guy who said I just explained cutting was just a fucking moron, nothing can fix that.

[@YusufOthmanSheriff]

Thanks so much, I never had any idea of CRISPR is about

[@phil.4688]

I just used a variant of this to cut the links in my brain that knew 'stuff' about CRISP-R and "fixed it" using the explanation that was given to Lex. Much better, awesome explanation. Thanks for the assist, cousin! :D

[@mitcr6078]

He means non homologous end joining when DNA repairs itself naturally. However, homology directed repair is considered a DNA repair mechanism when you introduce a repair vector - an insertion of a piece of DNA you choose

[@ericbriggs7383]

Manolis is so damn good at explaining complex shit like this.

[@alpineflauge909]

thank you

[@nancyling8976]

I finally understand how CRISPR works.

[@coconutfleetsleeper5717]

A concrete wall built by someone else is like a blank canvas to a graffiti artist, why buy expensive canvas if there's already premade available ones? some consider it art, other vandalism.

[@galaxy999in]

Wonderful explanation. Share and forward as much as possible. The birth of a new world.

[@noclue30]

Or the end of the world. How ever you want to look at it, because we are so caring as humans. Lol..

[@noclue30]

This all sounds fascinating, but not knowing much about it. Sounds like one more thing that could eventually end humanity.

[@gemini2in1]

Sounds like it. Or a way to wash a race out of existence or modify a race into existence.

[@noclue30]

@@gemini2in1 Lol.. or just screw it all up.

[@gemini2in1]

All in all. Nothing good in the end is coming from this

[@clray123]

The unsettling thing is that it's fkin around with systems we don't fully understand, and the consequence of getting it wrong is people getting sick or dying. It's a bit as if a software engineer started changing some important production code here and there to "maybe" fix some problem, with no backup or rollback plan whatsoever.

[@clray123]

@Raw Engineer For example, imagine a virus which does not really hurt people with Asian genome all that much, but wreaks havoc in other populations...

[@nicolasj8550]

CRISPR is already been used in clinical trials, and has shown some promising data with somatic cell editing in sickle cell disease and beta thalassemia. Early data, but nevertheless it excites me.

[@HassanWorld]

Well said, check my channel for videos on CRISPR companies.

[@LM01234]

CRISPR is referenced in a kids movie called Spies in Disguise

[@Eblis840]

Will Smith is supposedly in Scientology interesting

[@hamooon]

He's great at explaining this stuff

[@herauthon]

and there goes C19 - cut by memory

[@crowlsyong]

What is the 'street art' of gene editing then?

[@patrickschissler5860]

If CRISPR isn't a technological development you find to be existentially terrifying (or at the least morally abhorrent), then you are not thinking on a deep enough level to be charged with the responsibility to develop, refine, and determine the moral validity of developing such technologies.

[@noclue30]

100 % agree Why do most say excited and fascinated. I only think very scary.

[@patrickschissler5860]

@@noclue30 Because people have been indoctrinated into the religion of Scientism. Almost nobody thinks scientifically anymore. Have you noticed that almost all scientists are absolutely abysmal philosophical thinkers?

[@pablobronstein1247]

How dare you criticize science, repent by reciting seven Sam Harrises and be amazed that you are living star dust. Not a being with a "divine" spark but star dust, and that's brave and beautiful, so sayeth grand disciple Neil deGrasse Tyson.

[@patrickschissler5860]

@@pablobronstein1247 Fittingly, Scientism happens to be a religion that truly lives up to and surpasses the stupidity and malice that the new atheists erroneously attribute to Christianity, Judaism, etc. They know nothing of these religions, and yet stare down their noses as they blindly reassure themselves of possessing the rational faculties to know better what is true. The religious use science as it was intended; to probe the mind of God. The new atheists create a dogmatic and rigid religion attempting to use science to disprove the existence of God. With so many stagnating fields (evolutionary biology, theoretical physics, etc.), you'd think they would catch on that they were going about the whole 'apprehending reality' thing incorrectly.

[@ericf9479]

its so funny that jogurt can bring so much knowledge

[@pablobronstein1247]

This can go only right, I can see it.

[@melodydeese1964]

Hope it is good, I’ve bought some of their stock. Maybe I should get more. Hope it will be successfull. I need retirement money.

[@tomlloyd9364]

BEAM is crispr 2.0 though.

[@nicolasj8550]

I am long CRSP and EDIT for a few years now, keeping my eye on BEAM shot up lots in the past few weeks.

[@tomlloyd9364]

@@nicolasj8550 same here. Beam is better except if you need a package of new dna, then you’d go the crispr or car-t routes.

[@tomlloyd9364]

@@nicolasj8550 don’t forget NTLA

[@AtlasReburdened]

Ahh, so the beauty of CRISPR is that it's infinitely more accessible as a method of bio-terrorism. Here's a simple question I'm sure no bio-terrorist would eeeever ask. What happens if you modify a retrovirus to integrate a CRISPR 'kit' that seeks out the sequences responsible for building the molecular machines that do the repairing?...

[@CreatureOfGoddess]

Article acceleration of natural selection by trying to simulate exponential growth

[@TheEmergingPattern]

I would love to get my Vitamin C genes back on track! Can you fix it for me?

[@saturdaysequalsyouth]

So we will be gods?

[@juanfernandez1696]

Yes they just haven't figured out who's going to get all the money from it.

[@noclue30]

Bible say we are gods in the making a co-creator with God.

[@saturdaysequalsyouth]

@@noclue30 Of course it does, we wrote the bible.

[@noclue30]

@@saturdaysequalsyouth Very true, but i believe everything i read. That is why i write it all before saying it.

[@davidjensen2411]

Anyone remember the beginning of the film I am Legend?... #RefuseTheDnaInhibitor

[@sickmundb9828]

who owns CRISPR patent ?

[@kenkclam]

Broad institute 😂😂

[@cherokeerookie8973]

Guy has the Loudest gulps in podcast history

[@petersellers9944]

I love this Greek dude.

[@AndogaSpock]

Another way is to use Nanobots (not joking)

[@brandoncuster5219]

Unlexxxx oui use a natural Custer Cutter like moi 🤣

[@ronymararenhas931]

Cure HIV 🙏🙏🙏🙏🙏