Cytotoxicity Assay

Here you will see how to perform a cell toxicity assay using the InCell Analyzer and two fluorescent dies, Propidium Iodide and Hoechst.

[@arnoldfederico3030]

Would the highest concentration in the cells after adding 50uL into 100uL be 66uM and not 33uM?

[@shyworthacademy4151]

I need protocol..

[@AmirSabaghian-x7u]

Great idea, Please keep on this good work.

[@AnneAnne506]

But DMSO in cell culture causes cytotoxicity in comparison to cells with no DMSO or treatment as a control too.

[@kosheeka]

Yes, you are correct. DMSO exhibits cytotoxic effects. Compared to untreated controls, it can disrupt cell membranes and induce cell death, necessitating careful optimization of its concentration for specific cell lines and experiments.

[@mgjimvel]

Thanks!!!!!!

[@elcylambertua]

Thanks for the video! How do you film it, do you have a special hat ?

It’s all done with a Go-Pro camera. There is a head band that you can attach the camera to, so whatever you are looking at, the camera will point in the same direction.

[@elcylambertua]

@ thank you !!!!