DIY-TEC: LC Jar Lids (The Mushroom Network)

[@TheCombatKitchen]

Great video! Really enjoyed the thorough walk through. I did have a question: Why do you need the lure lock if you seal it with silicone?

[@TheMushroomNetwork]

Hi there and thanks for the feedback! Great question! Honestly this has been the most successful for us. We have tried several other DIY versions but adding the heat silicone helps secure the luer locks from being disturbed when you go to draw your liquid culture. We will be doing an updated video that will explain things in better detail and will be in proper order when drilling the holes.

[@ChuckFreeman0102]

Question about your syringe method. When do you put on and take it off? I know it won't fit in the pressure cooker or I imagine we could leave it on. Thank you and great content.

[@TheMushroomNetwork]

Hey there! Thanks for the question. We do not pressure cook the syringes themselves. Instead, we use single-use sterile luer locking syringes at the time of transferring. To protect the hole, we recommend using a DMF luer lock cap that fits onto the top just like the syringe would. Another option is a stop cock, but do note that it’ll add a few inches to the headspace. If you’re looking for a cost-effective solution, micropore tape works wonders. And, of course, don’t forget to cover with aluminum foil when pressure cooking. I hope that clarifies things! Let us know if you have any more questions and have a wonderful day!

[@ChuckFreeman0102]

@@TheMushroomNetwork Thanks this stuff is wild and your advice is truly appreciated. much love

[@ShouldaWaved]

I heard those style ports arent as food as the hockey puck foam black ones that are sticky, any thoughts

[@TheMushroomNetwork]

Hey there, Thanks for reaching out about the ports! We've actually experimented with both the rubber and the hockey puck foam black sticky versions for our LC jar lids. Both styles have their merits, and it really comes down to your usage and wear patterns. Needle gauge is also crucial, as repeated use can start to wear down the self-healing ports. We're excited to share that we have a new video, DIY-TEC: LC Jar Lids 2.0, that's currently navigating its way through our Myco-Verse Pipeline. This upcoming release dives deeper into this topic and offers additional tips and tricks for crafting your own Myco-Lids. It's shaping up to be a great resource, so stay tuned! Finding what works best for you and what materials you have available is key. Thanks again for your comment and for being part of our community. We can’t wait to share more with you soon!

[@RubenGarcia-kd6ff]

Where do you buy the luer locks?

[@TheMushroomNetwork]

Hey @RubenGarcia-kd6ff, Great question! Luer locks are indeed commonly available at medical supply stores in bulk. However, if you're in need of smaller quantities, we've got you covered! We offer them as part of our inventory, tailored to meet the needs of our community without the necessity of a bulk purchase. Feel free to check out our selection and find exactly what you need for your projects.

[@davideluciano7761]

What flow hood is that

[@FredzDeadBaby]

Amazon has these for about 130

[@TheMushroomNetwork]

Hey there! Sorry for the delay in responding but this is actually an electronics dust remover, and isn't really at true flowhood. We use this system for demonstrations as it has several LED lighting settings that make it very easy to see what is being done on the camera. That isn't to say that some people do actively use this and similar devices with great success. We just want you to be aware that this is not technically a proper mycology flowhood and we personally only use it for demonstration purposes. Happy foraging!

[@MycSailor]

Next thing you’ll be putting these on your agar plates.

[@valp729]

Hu. we have 3 holes. One for injection, one for 0.22 filter, I really do not understand what is the function of thr third hole

[@TheMushroomNetwork]

Thank you for your great question about our Liquid Culture Lids and the presence of three holes in the design. You're absolutely right; technically, you don't always need three holes as long as there's a sterile method for adding/removing contents and a proper ventilation port. However, in our specific design, each hole serves a distinct and important purpose: Self-Healing Injection Port: This is used to safely initiate the liquid culture. It's designed for easy and sterile access, ensuring that contaminants are kept at bay during the injection process. Luer Lock Bulkhead: Positioned at the center of the jar, this feature is used for withdrawing the liquid culture. It offers a secure and sterile way to extract the culture when it's ready. 0.22 Micron Filter: This filter is crucial for ventilation. It allows for gas exchange while filtering out potential contaminants, maintaining the sterility of the culture environment. These components not only make our Liquid Culture Lids highly efficient for their intended purpose but also versatile enough to be used in other systems we offer that utilize similar parts. And exciting news - we have just completed recording our 2.0 video, which delves into these details even more comprehensively! This video is set to launch later this week, and it will provide a clearer understanding of the functionality and advantages of our Liquid Culture Lids. So, stay tuned for its release, and thank you for your interest in our DIY-Mycology Projects!

[@WhiteBeardHashtag1205]

Out of curiosity, why do you call it "tec"? The rest of the community calls them "teks". Just found it odd, and figured I would ask

[@TheMushroomNetwork]

Hey there! Ah, the great 'TEC' vs. 'TEK' mystery. Here's the scoop: it was actually just a typo in one of our early videos. We've been updating our content to use the more commonly accepted 'tek' terminology. When we roll out the 2.0 version of this video, we plan to correct this and share some additional insights and tips we've picked up along the way, like how stopcocks have been a game-changer for us. Thanks so much for pointing it out, and for your curiosity. It's great to have keen-eyed folks like you in our community!

[@SparQz]

But when you unplug your syringe it will suck air back into the jar.

[@TheMushroomNetwork]

Thank you for bringing up an important point regarding the use of our LC Jar system with luer locks. You're correct that unplugging a syringe directly from the jar can sometimes create a vacuum effect, inadvertently drawing air back into the jar. To address this, we recommend integrating a stopcock into the setup. The stopcock acts as an effective valve, allowing for more controlled airflow and reducing the risk of unwanted air intake. Additionally, you can customize the setup with a length of 1/4" tubing that suits your needs, providing further flexibility and control in managing the pressure inside the jar. This combination of a stopcock and tubing not only mitigates the issue you've mentioned but also enhances the overall usability and efficiency of the LC Jar system.