DIY tissue culture but it's my *most expensive* plant

[@jakes.4517]

Lion’s mane mycelium is always kinda sus looking, but it looks totally fine. You can usually tell contamination because the mycelium will form a boundary with the contaminant

[@Jay7Rocka]

Lions mane even if it has a bit of contamination is usually fine.

[@joshrempel2076]

agreed. Lions mains always very wispy and pathetic looking. But it's very resilient, think I've had a bunch of cultures of a few different hericium sp. that's 3 years old at this point. All show great life when tested on agar.

[@rrrajlive]

Watching this just like a meditation/zen music. I'm a PhD with expertise in tissue culture, genome editing, etc. Although I'm not learning anything new here, but i like your enthusiasm. Wish you all the best.

[@digitalportraitist]

You exemplify why I have never and will never hire a PhD. What an arrogant ass.

[@aidennymes6335]

welcome to the mycology family

[@cameronl9698]

keep up the good work!! you’re videos are fun and educational to watch! I grow mushrooms for fun and its cool to see what else I can do with all this equipment! I’m looking forward to your next video and all the mememememees

[@DaltonEMain]

Great video! I love seeing the care you put into the process. I'm pretty envious of all of your equipment! XD

[@sparklessparkles728]

I support your refusal to do any extra steps! Another great video!

[@pobjuice4683]

for myc as long as its not green, blue, or pink (unless ur growing pink oyster) ur good c: im gonna join the discord server eee im so happy you shared your dishes

[@stoian9]

I love that you edited out flipping off the lid you hate but left just enough so we know it happened.

[@Anonymous-m9f9j]

yay my favorite comedy science show

[@michaelvonfeldt9629]

Lol always hilarious and entertaining as well as informative.

[@larrygeran6964]

Wow, I would definitely look at your node! Your shirt is fire

[@jkoblivion4175]

All hail The Tissue Culture Queen!

[@nackedgrils9302]

Ouch, I feel for you, I just found this plant for less than a $100 in Canada and I had no idea it even existed. Epipremnums are quite underrated!

[@ryan_roga]

4:09 I feel like hands are always going to be dirty and add contamination. Would it be better to do that with a clean container?

[@opipico9144]

great video, do you know where to find the cloning protocol for Salvia Divinorum?

[@charliemagoo7943]

Have u tried research gate or Google scholar???

[@lucash7012]

Awesome vid! Where’d you get your laminar flow hood, how much was it?

[@plantsinjars]

It’s from Air Science but I don’t remember the exact $$$

[@amfy7616]

I so so love your content, you have me want to get into tv myself! I'm just worried about the phytohomones, especially the auxins. I really wanna do tc but I don't want to risk my health...

[@plantsinjars]

As long as you handle them carefully, it's not a big deal! i.e. wear gloves and I wouldn't store them near my food, but I also have more health anxiety than most people lol

[@ChipsandIcecream37]

Is there a reason you use an overhead flow hood vs a horizontal one ? I'm in the market and was wondering what your think was.

[@EmilioRodela]

Could you do a video about your aseptic technique?

[@barcaisbest10]

Great content 🙌🏻

[@philup6274]

The decision to film with jet lag is why i suscribed ... Thats a boss move,

[@metasamsara]

Do you have a plan against viroids? I just learnt that iso alcohol increases propagation risk of viroid infections considerably.

[@markregan7639]

When me and my wife came back from Japan we both slept 14 hours lol

[@bradxwx]

I've only grown the magic varieties, so I have no experience with Lion's Mane, but I would be very suspicious of that culture. Mycelium is mostly always white. The only exception I know of is Pink Oyster mycelium. It's pink!

[@jaqhass]

If you culture just for aquarium plants and not sales, would a hotplate be worth it? Or is a stirrer without heat just as good?

[@plantsinjars]

stirrer without heat is perfectly fine. I rarely use the hot plate

[@marygorchidsmore2058]

Hi Lauren, great video as always. What are those plastic containers called that you grow your tissue caltures in? Are they autoclavable? Or do they come sterile?

[@plantsinjars]

The big ones are from Plant Cell Technology. They are autoclavable and reusable. I typically stack 20 of them together, roll them up in a pressure cooker bag, and autoclave them all at once to sterilize them (planning to show that process in an upcoming vid)

[@azamsheikh5]

What in side two orenge cap bottle

[@nicolemckay6022]

So I finally gave making medium a go, but I forgot to adjust the PH level. Should I scrap the whole batch or do we think it might still be worth giving a try? (followed your begonia tutorial - except the ph part ahhh).

[@plantsinjars]

Sadly you must restart 🥲 I’ve done the same thing

[@charliemagoo7943]

Annnnd. The big question. If you just add more hormone that might get killed by sterilization, how much more do you add.??? If pgr$ have an expiration date how much more can u put in to make up

[@weedlipe]

amazing video! where is the link of discord?? =)

[@plantsinjars]

discord.gg/nEs8aYts49 make sure to introduce yourself when you join!

[@TheGlitterBeeBoutique]

How many plants come from each node typically? Just 1? Or lots?

[@plantsinjars]

A lot

[@TheGlitterBeeBoutique]

@@plantsinjars so it's typically worth sacrificing a node? I have a gorgeous Monstera Albo that's pretty big so I might just give it a try.

[@Gts2pro]

Looks ok that lions mane

[@jimbasaysay4285]

where did you purchased your jars?

[@plantsinjars]

the deli containers in this vid are from plant cell tech

[@robertfrazee232]

Hope the Japan trip was good, Queen!

[@batmanofperth2647]

Hello fellow organisms😂😂😂😂

[@ericnyamu9981]

A leopard is not teaching tc . Wow . 😁😁😁

[@paulocoelho558]

Hi plant lover... Can't help but wonder, since you just came back from Japan, where's your kimono? Just trying to throw in a silly comment... Anyway, great video! Now, a question for the expert: What's currently considered the rarest plant in the world and could you clone it? 😉😜👘🪴

[@oakleydubois6726]

Ughh your stuff keeps popping up on my feed. I keep watching it. You see the problem right? I'm gonna have a full room of tissue culture equipment and be known as the crazy plant lady. The conversion has begun. This is your fault!!! I'm still trying so hard not to buy all the stuff lol.

[@plantsinjars]

I think it's a sign that you need to try tissue culture :)

[@lydialiu521]

I wonder if this recipe works or not, and Would love to see your follow up video of the results

[@plantsinjars]

I've used it before and it works but I'll make updates on my instagram as they grow :)

[@NaatClark]

Can't speak to Lion's Mane specifically but in my admittedly limited experience with culturing mycelium, "probably fine" usually means "should start over"

[@plantsinjars]

oh lord

[@iandangelo-o3d]

So if it were a different transgenic plant, with a different color like pink orange or orange with red dots then maybe I would pay 1.5000, but paying a high price for a natural variation I wouldn't do it.

[@spencercurtis86]

Does it take a long time to do succulents? I can donate the tissue haha

[@plantsinjars]

Succulents grow really well in TC. I have some echeverias in TC rn

[@Sama_09]

Wait how to identify if my plant is not epipremnum aureum !! 😂😂😂

[@josephfcarrillo]

Is that engagement 💍 real? It is Massive!! 😮

[@Anythingforfreedom]

I see $10 books listed on Amazon for $700 from time to time. This is a bit different.

[@turbidPumpkin]

Hi Matt

[@Planty-Mandy]

Hi Matt!

[@lolgh4499]

bye Matt

[@sjoer]

99% of these varieties are found on the side of the road. don't fall for Big Variety Inc!

[@sjoer]

Isoproperol? :D

[@michaelcollins8650]

It's ironic you are allergic to everything, and yet grow plants..

[@GabrielSilva-fr9fk]

reach out im starting my lab, and need real face time experience talk. pick ur noggin'

[@fanyoktavia1703]

? what make those stupid plant so expensive? its a weed