DNA Extraction Protocol - Part 2

[@gubatron]

it'd be cool to have a video like this but which would explain (briefly) the "why" of each step.

[@Jumanji98]

You think they know that? They can't even image DNA clearly enough to see anything of note. Science has been hijacked since the mid 1850s. Good luck finding a paper that leaves no doubt in your mind that the mystical double helix exists, nevermind a paper proving DNA/RNA protein synthesis is occurring as the many animations portray.

[@JatnaRD]

This video is so much in first person view! I wonder, where in the world are you placing the camera? In front of the technician? It is a great angle.

[@avianbirds]

probably slightly further and then zoomed in

[@christianmontoya7033]

or maybe they just used a go pro

[@xerxes_sama]

Why did we wash the DNA pellets with 70% ethanol

[@gowthamm7200]

this video describe the stepwise learning and it can be describe the step program

[@afreenkhan4998]

before Dna extraction how can I form pilletes from fungal culture??

[@jhonmarkcecilio8028]

Hello Good day can i get a softcopy of list material and reagents of this protocol thanks 😊

[@Heyygurlitsme]

Wish I knew what all these tools were called

[@oenologist]

very helpful

[@aminlone6330]

Orchestration is missing in the protocol

[@kalilohazemba2589]

Very helping

[@ahrorbekkarimov4731]

Best👍🏻

[@Ann-un9nh]

Please add closed caption in your videos. Thank you!

[@croschko]

What If you spill the Dna solution on the Table, how to remove It, with a towel? What If your gloves have a hole and you get DNA on your skin, what Happens? Are there Information Materials for what do to when Things Like this Happens ?

[@kanmanisabapathy102]

What happens when the DNA touches your skin? Is it dangerous?

[@atirahabdullah4414]

Hi! Asking your permission to take some of your video content for education purpose.Tq in advance

[@amalibrahim8503]

What is function of vortex?

[@AA-wd2or]

A vortex mixer, or vortexer, is a simple device used commonly in laboratories to mix small vials of liquid. If sample sit long time can be to stiff , so you mix it to relax it.,It consists of an electric motor with the drive shaft oriented vertically and attached to a cupped rubber piece mounted slightly off-center. As the motor runs the rubber piece oscillates rapidly in a circular motion. When a test tube or other appropriate container is pressed into the rubber cup (or touched to its edge) the motion is transmitted to the liquid inside and a vortex is created. Most vortex mixers are designed with 2 or 4-plate formats, have variable speed settings ranging from 100 to 3,200 rpm, and can be set to run continuously, or to run only when downward pressure is applied to the rubber piece.

[@MrOrangeQueen]

Why should anybody try air-drying those DNA-pellets, if the workplace is not sterile? lmao btw: It wont take 20min to evaporate a tiny drop of 70% EtOH

[@raahul1303]

If you're that, worried do it in a biosafety cabinet

[@MunsKi]

ok where is the dna?

[@Jumanji98]

This particular field relies heavily on the "trust me bro" model.

[@НикитаМаксимов-т2я]

Calm night 81

[@mohamedgalal7863]

It's a very slow process

[@bloominglotus6280]

MUHAMMAD galal

[@markwillentdion]

Mark Zuckerberg, DNA

[@mandeepgill8861]

Ppllllll,,,

[@smallbeginnings3497]

Any reference papers for this protocol so that we could understand the biology behind the steps. Please provide the link to that. Regards.

[@ghinwaosman]

@eternal footman DNA isn’t always in the supernatant, it can be in the pellet or the supernatant (depends on the solvent you’re adding before the centrifugation)

[@ixayla]

Why did you discard the supernatant this time, not the pellet?

[@lilna7444]

Because pur ethanol precipitates DNA, thus the pellet contains DNA, and we want to keep it ;)

[@gilian2587]

The centrifuge separates heavier material from lighter material. The first time through the centrifuge, the dissolved cell walls and larger cellular structures were clumped to the bottom. The second time through; the only two components left were the ethanol and the DNA, and DNA is heavier, so it clumped to the bottom the second time. (When I say heavier in this context, I mean 'more dense')

[@arjunpal8340]

Isn't the ethanol supposed to be ice cold?

[@primawidagdo7679]

What is the function of cold ethanol?

[@arjunpal8340]

@@primawidagdo7679 Ethanol changes the dielectric constant which causes the DNA in the solution to precipitate out. The coldness is just to keep the temperature of the solution low.

[@hmmwila]

My thoughts exactly

[@ot8479]

It's all great and good.Thanks very much, BUT that loop going on, and on, and on, and on, and on.... is driving me nuts. Maybe no music is another option. I mean, repeating a loop to infinity doesn't make it more listenable.. Just saying..

[@Ideal_cmls]

Is spin column tube not used in dis extraction process?

[@elenaabbott2693]

im sooooooo dumb lol

[@سبحاناللهالحمدلله-م8ح]

ياليت يكون بالعربي

[@maltreated1]

I can help you if you need

[@hadiadouar7595]

@@maltreated1 hey can you help me as well!

[@maltreated1]

@@hadiadouar7595 tell me what you need exactly, and I can explain it. With my all pleasure

[@hadiadouar7595]

@@maltreated1 i mean if you’re good with biology molecular (SEQUENCING ,PCR and RT PCR , CLONING..etc) i have a lot questions and your help would mean the world to me