DNA Microarray synthesis

[@sanjuktapaul6176]

Sir, because of your tutorial videos, I got first class in my M.S.C. Thank you sir!

[@shomusbiologyofficial]

That's a wonderful news. Congratulations

[@강아지-m5j]

Congrats!

[@tehreemfatima1776]

@@강아지-m5j .............. mm

[@nandinichaudhary2962]

M. Sc which subject?

[@malaikasubhani4341]

I’ve no words literally!! Istg your videos are so informative and i passed all my exams just bcz of your lectures. Your skills of describing the thing are excellent and superb! Lots of love from Pakistan. I owe you alot, thnk you so much.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@DamjanManevski]

I'm currently prepping an exam for a MSc program at TU Munich in Germany. This was a super nice explanation, thank you very much!

[@Singh-j5g1s]

The bestest and the easiest explanation i ever got

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@cathieangella3708]

You really God sent. You make my learning to easy. Stay blessed and knowledgeable. Thank you

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@himanshugupta9656]

I am here exactly after 2 years from the date of the being uploaded....thnks for the descriptive lecture in short duration cheers :)

[@shomusbiologyofficial]

+Himanshu Gupta thank you. Glad you liked my lectures

[@daizydaizy6079]

Shomu's Biology

[@nooriyajameel8035]

Absolutely amazing the way you talk and try to explain each n everything briefly, kudos 👏 It's been 5 years now thay i have started studying again,i took a gap of 5 years so i had difficulty in understanding many things,but thanks to you.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@funnywaves8765]

Thanks from Bangladesh. You are doing a great job man. :)

[@shomusbiologyofficial]

+Funny Waves thank you.

[@sriradhaganguli7660]

thank you so much... it gets easier when u teach.. n fortunately u have videos on most of the topics we require.. great job!!

[@shomusbiologyofficial]

+Sriradha Ganguli glad to hear that. Keep enjoying lectures

[@kainatjameel8992]

What a wonderful teacher he is❤️learn a lots from him❤️

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sadiamumtaz5057]

Iam a big fan of u sir i learn alot from ur videos keep it up and God bless u ....

[@shomusbiologyofficial]

Thank you very much.

[@sadiamumtaz5057]

my pleasure...

[@bijayalaxmisahoo7541]

plz sir add a lecture on oligonucleotide synthesis

[@bts_rollercosterlifepurplearmy]

U just show me different angle of Microarray technique 👍👍

[@shomusbiologyofficial]

You're welcome

[@강아지-m5j]

Thank you. This helped me understand microarray experiment itself.

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@muhdshafiq3597]

you make it seem so easy bro, thank you

[@user-zh4mu7ki4t]

U are the best sir.. Thank you for explaining..

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@tithibhattacharjee9104]

Your explanation are the best sir ❤️👍

[@shomusbiologyofficial]

Thank you

[@suraali4108]

Thanks a lot for this easy-to-understand video!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@divyasingh-jq2rh]

Thankyou so much Shomu . Your video tutorial helps a lot in understanding subtle nuances of the topic .Learning Simplified by Shomu's Biology (y)

[@shomusbiologyofficial]

+divya singh thank you. Glad it helped

[@gaurikavimandan3597]

Very nice... Thanks for the simplified explaination.

[@shomusbiologyofficial]

You're welcome

[@mayukhroy5393]

This Video was very helpful Sir.

[@shomusbiologyofficial]

You're welcome

[@alihussainallawati5743]

very interesting teaching on microarray experiment. Thanks.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@SaintNath]

Very easy to follow explanation. I think you could have made it a little bit shorter, since only the last 5 minutes are important for understanding the in situ approach, but when you fast forward through the video it's a quick way to relearn the principles. Helped me for my exam, thanks!

[@aboutstudies123]

Thank you very much Sir. It is very helpful and easy to understand.

[@rrsrk2424]

Marvelous .....Thankyou very much sir.

[@shomusbiologyofficial]

You're welcome

[@princessesjan2780]

Great job Understand very well Thanks

[@shomusbiologyofficial]

You're welcome

[@nooruikhuda3188]

thanku so much sir .... respect from kashmeer

[@kugelblitz7832]

Thank you Suman, much appreciated

[@sajibroy1917]

What a beautiful lecture sir, thank you

[@shubhamkundu6154]

it decreses ur level

[@priyankaparihar5954]

thanks shomus!!

[@chandhoksahil]

The microarray grooves will also require free OH groups to which new nucleotides can be added, right? The blocking agent blocks these OH groups, right?

[@archijain744]

Sir , this video is very helpful but can u give one more lecture for microarray methodology

[@hinaraja7612]

Please sir answer me ...I've to present this topic tommorow I've an question sir ... If we have to remove the blocking agent from 3 unmaskes boxes then why we had added that blocking agent in those boxes?

[@asmafatimah1881]

Why the mask and blocking agent both are used? Both have the same purpose not to add nucleotide.. Let suppose why we can't only use mask as mask will also not allow the nucleotides to bind with grooves.. We have to remove the Blocker everytime before adding any nucleotide.. then Why we can't skip Blocker? And 2nd thing why blocker is added with every nucleotide which we are adding in the grooves.. Kindly make it clear plz

[@asmafatimah1881]

And we do we rinse the slide? I mean all the material may also remove with water😓🤔

[@shekarsubramanian9562]

Sir I have one doubt - If blocking agent is going to stop any further nucleotides from binding and masking is also going to stop any nucleotide from binding, then why add both?

[@prekshavashistha3955]

please sir ...give a short lecture on dna microarray methodology

[@iamnotblindbhakth1538]

please explain Radioisotope tech such as Geiger Millar and scintillation counter tech and tracer studies etc

[@priyaslife9603]

Very very useful video sir

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@verogart6177]

I always understand with your videos! Thanks!!

[@shomusbiologyofficial]

+VeRo GaRt thank you. Glad you liked my lecture.

[@venkatswamy6440]

GOOD LECTURE

[@hirenpatel2553]

Which chemicals are used as blocking agent and why ? What is mask and how to cover whole chip by mask ? How and why gaps are there ?

[@lucygomango899]

Tq so much sir .....💐💐💐🙏🏻🙏🏻

[@shomusbiologyofficial]

You're welcome

[@akashchobe8749]

Thank you sir ...u much very helpfull

[@dakshitagarg3734]

Sir can you please tell how can we analyse hormone through microarray for example dopamine

[@slguy501]

You explain very well. . Almost all our batch-mates watched ur videos on chromatography. .

[@violason811]

I m ur big fannnnnn sir....u r just awesome

[@shomusbiologyofficial]

Thank you

[@sabakiran3162]

A scientist designs an experiment using a yeast genomic DNA array. The scientist wants to compare gene expression between two samples of the same yeast strain grown in hot versus cold temperatures. Which best describes the experimental conditions? Plz ans Sir

[@gayathrisenanayake4988]

Thank you sir for the nice explanation given. Also can you explain what is called the curse of dimensionality in micro array?

[@vaishalithukral9129]

Sir what is the purpose of adding blocking agent if we can block the addition of nucelotides by simpky adding the mask

[@khushbuislam6941]

A very good lecture..

[@vineetajoshipant4865]

Pls lemme know, Whether a RNA can be hybridised with probe cDNA previously immbolized, and can we detect such hybridization?

[@juliawang3055]

why do you need blockers to begin with

[@jayaprakashc.a2941]

Very interesting class

[@shomusbiologyofficial]

Thank you

[@manishayadav-oc9rp]

Sir u are great

[@shomusbiologyofficial]

You're welcome

[@Kawka1122]

Very interesting. Thank you.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Kawka1122]

@@shomusbiologyofficial by the way, do you have( or plan to prepare) lecture on solid base oligonucleotyde synthesis (like the one from Thermofisher) for synthetic biology ? How those machines function? From what I read process is similar, although they deprotect with chemicals rather than laser, but that is not my area of expertise :) so I may speak gibberish xD

[@proneyex]

Great videos. Thanks! Just wanted to ask how do you learn so many concepts? Is it through a lot of self study, research and reading text books?

[@Hussein_Reveiw]

Thank you Mr 🌹

[@shomusbiologyofficial]

You're welcome

[@Unpopular-channel]

can you explain Randox biochip array technology

[@aheedan9957]

Can you make some videos on disease modelling and disease mechanism? It would be so helpful. Thanks

[@mubinmirza9741]

Is it oligonucleotide microarray?

[@mrwolf31]

Hi Shomu, I have a question. Do you know how the Micro-array slide is prepared? I have a Micro-array scanner, but I can't seem to find information on how to get a specific type of Micro-array slide. I have found some slides, but they seem like regular slides.

[@shomusbiologyofficial]

+U tube You I have a video on that also in my channel

[@shubhanginiraj284]

Sir , i hv query that , at one tme we hv to remove only 2 different blocking agent at different blocks

[@yuvaneshv2117]

is this part of any playlist or just a single video? thanks

[@manishamathur3941]

sir i have a ques when we are using ssDNA then what is theneed of fabrication of chip .... i mean sysnthesis of probes

[@shrutishukla722]

Can you make vedio on intragenic crossover in rii locus on T4 phage.

[@denischeptim5447]

i love your tutorial sir

[@AS-ct9yq]

bhari hai boss...😎😎

[@kajaldhawan7735]

Sir you do a great job please make vedio on animal biotechnology full topics of bsc final year 5 sem biotech

[@nguyenanthuan8730]

Dear Sir, thank you for your video. Could you please let me know the chemistry of this synthesis? the chemical structure of Blocker and Mask? Thanks

[@mujahidiqbal4822]

really a nice concept elaboration thnx,

[@shomusbiologyofficial]

Thank you.

[@manipanigrahi4308]

sir, as you said that each nucleotide which is added has a blocker.... so how come the next nucleotide can be added to it in the same groove?

[@tonial5789]

i have the same question. I have noticed a few times this guy is not clear on such details

[@hariprasath7652]

Plzzz... Make a video about protein nucleic acid interaction

[@dildarhussain6396]

thanks sir g for such a leacture

[@shomusbiologyofficial]

+dildar hussain glad to hear that

[@anjubhargav1144]

Osam explanation

[@shomusbiologyofficial]

Thank you

[@srikrishna2258]

Hai sir please suggest how design a work/ project title

[@sanzharaitbay7267]

So there only 760bp in the subpart of chip ? But genes are actually far larger ...

[@sanzharaitbay7267]

kokoko

[@hunnykakar2822]

Thank u so much very helpful

[@dr.hemalpatel2170]

How to use microarray for genotyping....??& How we use this genotype data to produce phylogenic tree .......plz plz plzzz explain.....

[@priyaslife9603]

Pls ecology evolution and diversity se aur video banay sir...jo NET k exam k liy helpful ho

[@shomusbiologyofficial]

Sure

[@cynthianaeem]

I requested you to please made video on homopolymer tailing but still I m waiting 😓😓😔😔😔😫😫😫

[@asmafatimah1881]

I have a question and I need to know it today.. can any body help me?

[@shikhagill555]

Sir dna Microarray and DNA microarray synthesis is the same

[@krishnapatel-qn8on]

can u pls explain more about micro array pls

[@danishbhat9260]

Which type of laser is used to knock out that shit

[@piyalidas5286]

pls make vidio on biochips and biosensor

[@shomusbiologyofficial]

You're welcome

[@meharkhan521]

Protein and dna arrays ka full concept py video bnae??

[@shomusbiologyofficial]

Ek video se nehi Hota. Pura molecular biology samjho

[@AdityaRaj-zk9eh]

thanks sir

[@shomusbiologyofficial]

You're welcome

[@swatigavimath85]

Sir, CDNA is double stranded, how it will bind to oligonucleotide in microarray?

[@priyankamanuja1617]

It is single stranded complimentary DNA

[@monismukhtar4424]

Anyone there?? i have some queries about microarray topic.. pls help me

[@gasperkosmac7672]

thank you man

[@TechnilTechsil]

when You can"t confess confuse them .

[@gyanikashukla8315]

The concept is not clear. You made it too much simplified.

[@thedefender7087]

he simplified so that u can understand it well...

[@myimmortal9488]

اهم شي استاذ محمود يرضى عليه xD

[@irshanaureen7847]

Anyone else in 2019

[@discouniverse]

wrong explanation...mask is used to selectively unblock, but if LASER is used then you do not need a mask

[@MySiva01]

stupid discounviverse

[@MySiva01]

stupid wiki is not a good reference

[@discouniverse]

fornix eagle yes you are stupid...go find good source for you and read you moron

[@bnravindra1989]

Nyc

[@shomusbiologyofficial]

Thank you

[@poojashivhare2872]

please ise Hindi me smjhaie

[@ML8ooof1]

how the hell you know every thing??

[@shomusbiologyofficial]

I don't know everything. Just sharing little facts

[@ML8ooof1]

Shomu's Biology Honestly, I’m really impressed by you. You look too young, yet you know ALOT.