Gene expression analysis

[@googly83in]

I am a pathologist. In 3 years of residency,no faculty or book was able to explain molecular techniques in the crystal clear simple way that you do. Anyone can make a presentation super tough and intimidating. Real knowledge lies with a person who is able to come down to the simplest of the terms and explain something as alien as molecular diagnostics so easily.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@robindex360]

U r the only few persons in youtube who is explaining deep topic of biology at best in regular serialized manner..it helps many students...Thnx

[@sanjanapandey882]

You have got the gift of teaching sir. Not everyone has the ability. More power to you.💚

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@fedDuke94]

In 15 minutes you have helped me to understand this topic more than my university lecturer did in 1 hour. Thank you sir, sub earned

[@taliaroman4564]

Thank you a lot. I'm psychologist and when I need to understand something about molecular biology I look for you. Congratulations! You explain very well.

[@guilhermebartolomeugoncalv6497]

I'm a pHD student from Brazil. Thank you for sharing great content, sir. I've been learning a lot w/ you.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Stay tuned for more

[@sarahaddad4279]

Why have I not found you before this point in my life? Thank you!

[@ekimkara4503]

This is an excellent review before the midterm! Thank you very much, I had already studied the material but you made me notice some of the spots I have been missing-- I hadn't paid attention that hybridization occurred with the cDNA and the complementary cDNA strands we synthesized. It makes even more sense now. Thank you. :)

[@yt.abhibhav]

I am a data scientist trying to get my hands in biology and your videos are helping me invaluably. Thank you very much

[@TheCherrytree123]

thank you! I major in IT and about to start Bioinformatics research. This is best place to learn

[@jayaprakashc.a2941]

This speaks about your depth in knowledge. Wonderful class

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Jubokay]

Omg 😳 10 years old video and today, you look the same. Btw best video, watching after 10 years, still best video so far

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@nixi7688]

Shomu, you're a legend!

[@Latentleo]

U are awesome sir...i was struggling for this topic and not understanding at all from good books like kuby ...bt you made it so simple....

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@yahyam7170]

sir .. i dont know how to thank you .. you made it very easy to understand

[@abumohammed9659]

Dr. Shamus you are amazing thank you I have understood the microarray after this video. I hope you keep up doing beneficial videos especially in Plant Biotechnology field thanks

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@ravinaa315]

One small correction: Cy3 is a red colored dye, while Cy5 is blue. When excited at a proper wavelength, Cy3 fluoresces and is seen green while Cy5 is red

[@anupamameher8330]

Yaa dts rytt

[@KountayDwivedi]

I'm a research scholar. My area of research is AI in Healthcare; n believe me, so far most of the concepts that I have cleared, are due to your explanation. Thanks a lot. :-}

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@kamithagunarathna6421]

Wow! Superb Explanation! Thank you so much and Wish you all the best!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@misslaaaura]

Thank you so much Shomu. You save me for my genomics courses.

[@nooriyajameel8035]

Mine too😂 else i wouldn't be able to get even passing marks in genomics!

[@andrewrichards2572]

You're a legend! Keep up the good work.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@liujiangping9086]

Thank you so much. It's so helpful for an interdisciplinary student.

[@shomusbiologyofficial]

You're welcome

[@abhisheksinha6969]

Such a beautiful explanation of a difficult topic like microarray analysis. Thank you for such content.

[@shomusbiologyofficial]

You're welcome

[@kinkinib]

You have lovely videos Shomu,thanks!

[@shomusbiologyofficial]

+Kinkini Bhaduri thank you. Glad you liked my lectures

[@mailaralingams7896]

excellent sir ... u r saviour

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@inesgonzalez2246]

You're saving my life 🙂

[@shomusbiologyofficial]

You're welcome

[@gdp2102]

A very good job Shomu. Gd luck

[@shomusbiologyofficial]

Thank you

[@desithugbaba9136]

M a pathology pgt ... lv ur video. Thanks a lot

[@markmcdonald1371]

Excellent job explaining it.

[@shomusbiologyofficial]

Thank you

[@dhahihusain6463]

Best ever explained,,, Thanks a lot

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@nidhi1454]

@@shomusbiologyofficial can you explain protein level gene expression

[@agentgunnso]

Amazing! how you easy explain, I was reading a scientific article. But your video saved me! know I understand it all! thank you so much!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@saman7032]

Thank you dear Shomu. Very helpful.

[@fips001]

blue is the new YOLO XD

[@rahayupertiwi4299]

You help me so much for my scholarship interview

[@shomusbiologyofficial]

You're welcome

[@habtishagripa5552]

my confusion have been removed Concerning to gene expression thank you my dear !

[@theloyaldisciple2543]

thanks shomu carry on the great job ur doing god bless u!!!!!!!!!!!!!!!

[@elkanahhaon1790]

Shomu you are awesome.

[@TheMaggiemagdalena]

do you have an explanation of SAGE? Serial analysis of gene expression

[@sohamomeni3701]

Thanks for your amazing video,it helped me a lot,🙏🏿🙏🏿🙏🏿🙏🏿🙏🏿

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@rajdev4507]

I just love the content.

[@shomusbiologyofficial]

Thank you

[@jayashreeugalat8636]

Sir, I am fan of your teaching, really your teaching is wonderful, you make very complex things to be understood by even very poor person in biological sciences. Really you are doing great service by doing such a teaching, people like us getting lot of benefits out of this.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@sewarkhassawneh9051]

thank you so much for your videos, I need help to understand overexpression and misexpression

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sewarkhassawneh9051]

@@shomusbiologyofficial can i have more explanation of over expression ?? Any reference

[@robelahmed1082]

greatly explained,,,,thanks a lot

[@shomusbiologyofficial]

You're welcome

[@BoomisTales]

Thank u pa...😊

[@n.dcreations2805]

Excellent lecture thank you sir

[@shomusbiologyofficial]

You're welcome

[@sujatasarangi6055]

In which book we get the proper note of it ..

[@samferrer]

Use Facebook live ... you are a very good lecturer ...

[@shomusbiologyofficial]

+Samuel Ferrer thank you. Glad you liked my lectures

[@deepthirao5378]

Another excellent presentation. Thank you so much!

[@shomusbiologyofficial]

+Deepthi Rao thank you

[@qaisarsbiology5267]

Sir amazing explanation but you said in the yeast mRNA extraction, the cDNA is single straned. I think cDNA is double stranded please correct me....watch 8:25

[@rafiquealangiyam3644]

Sir! Could u pls explain about DATA MINING

[@trishlabhati9738]

is gene expression analysis is the technology behind DNA chip..???

[@njana007]

Can microarray be used to find the differential functions of a gene?

[@cryptanoidexe.953]

Literally thankyou so much

[@shomusbiologyofficial]

You're welcome

[@hemantkumarsonawane9997]

Please make separate video on serial analysis of gene expression

[@lamia9466]

thank you so much, that is very helpful !

[@shomusbiologyofficial]

Thank you.

[@HunterDriguez]

Awesome video! So one would use 3 different chips in this case? If you are using two dyes, one for the control sample and one for the three sporulation time points, would you be able to compare expression among the three sporulation times?

[@naturalindia-ow6cz]

Excellent cover topic

[@shomusbiologyofficial]

Thank you

[@drtrpadi1]

Very nice presentation

[@shomusbiologyofficial]

+TIRUPATHI RAO Padi thank you very much

[@abiramijothiramalingam1453]

this video s very useful sir..

[@shomusbiologyofficial]

You're welcome

[@shahramniknafs6840]

Very very nice. Thanks a lot

[@Etrueyeei]

can you explain how dchip software works?

[@nidhi1454]

Can you explain what is protein level gene expression

[@Botanophilist]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@amonoracheal9118]

thank you for the video!

[@shomusbiologyofficial]

Glad you liked my lectures

[@nevi1708]

very helpful, thankyou

[@lamjennygrace623]

Thank you so much for such a wonderful video. I wonder is it correct to say that transcriptomics is the study of gene expression at RNA level? Why don’t we use proteomics to study gene expression at protein level instead because protein is the thing that make it happen, which is more certain than RNA - the thing that appears to happen?

[@lamjennygrace623]

and for non-coding DNA that does not code for any protein but only code for RNA, if that non-coding DNA is expressed, will they become rRNA and tRNA? In the case that I wanna study the transcriptome of food spoilage microbe, which produce spoilage exzyme, sbstances, do the non-coding RNA matter?

[@ttnot2005]

Thank you for SMART lecture

[@shomusbiologyofficial]

+Tesfay Teklehaymanot glad to hear that

[@MrsPixie-ni4lb]

YOU'RE AWESOME! THANK YOU!!!

[@shomusbiologyofficial]

+Vanessa Marroquin you're welcome

[@bhargabgogoi1692]

you mention that c DNA is single stranded... is the cDNA is single stranded?

[@baratchian]

No, it's not!

[@bhargabgogoi1692]

sir .. if the cdna is not single stranded. then how it is hybridiged with its complemenary RNA in case of study in gene expresssion.. plz explain

[@Zawaf]

You are confusing circular DNA with complementary DNA. cDNA is single stranded DNA which was reverse transcripted from RNA so we can sequence it. Circular DNA is what we call plasmid in bacteria, it's a double stranded DNA that is circular in shape.

[@migueltaranto9679]

I think you may be confusing terms, gene expression correlates with levels of proteins, and not with levels of mRNA, since there are processes of sequestration or degradation of mRNAs after their synthesis and exporting from the nucleus.

[@uzmamahar8804]

Thank you 😊

[@shomusbiologyofficial]

You're welcome

[@tasnovatabassom7612]

Thank you sir. I want to know about locus heterogeneity.

[@emkahuda776]

Thank you for the explanation. Im mathematics student trying to understand about biological things. Anyway, when you said more control genes and less sporulation genes, how do we know what type of control genes are there (os is control genes only one type?) Also, how many percentage of each types if there is different type of control genes?

[@happygarlic13]

not what i was looking for, but helpful nonetheless! You explain really well! I would suggest you specialise further in biology and teaching. Really nice top-down-thinking - never losing oversight over problem/task/exp./... at hand.

[@iffatazim6722]

Sir pls explain protein microarray and its types

[@ncertsciencebylaxman8502]

Thanks guru ji

[@shomusbiologyofficial]

You're welcome

[@shantanudas1884]

Please make one video on DArT

[@rafaelamachadotugores4769]

thank you so much !

[@shomusbiologyofficial]

You're welcome

[@nabanitabardhan7423]

Thanks a lot.

[@shomusbiologyofficial]

You're welcome

[@KevinLopez-rl6wq]

Do you have any videos that explain why measuring proteins is difficult in contrast with measuring mRNA? Is it because there are many more analytes in the case of proteins vs mRNA?

[@MrRisha100]

I have a question. DNA chip/microarray has ssDNA embedded, however, we make cDNA from isolated RNA, as mRNA is free from introns so how this cDNA bind to its respective ssDNA present in DNA chip because the ssDNA also has intronic regions. Please explain. Thank you

[@monikakennedy0012]

Sir can you please do a video on sage analysis

[@shomusbiologyofficial]

Okay may be later

[@monikakennedy0012]

@@shomusbiologyofficial thank you sir

[@guylainedoucet9634]

Thank you! you help me a lot. I have a question for you: after denaturation of ds DNA, we obtain two ss DNA (one that is exactly the same as the c DNA synthesized by adding reverse transcriptase to mRNA, and the other one complementary to c DNA), but if I put the two ss DNA to my DNA chip, the cDNA of my yeast expériment will hybridize only with the ssDNA that is not the same as the cDNA...so why do we put at first both ss DNA on the DNA chip? Guylaine from Canada (P.S you should teach at Harvard University)

[@1207sanjana]

Most amazing videos. Has always helped me through every course. Thank you so much! :)

[@lukisansuaraalam1274]

thank you for nice sharing

[@AliRaza-kw9dm]

When we put Cdna in chip so it will bind with dna from where that dna to which it will bind comes?

[@mazinalasadi1461]

Many thanks

[@bolesathvika3708]

Plssss explain in detail cap analysis, Cage

[@dr.wazihahmad786]

Massab, it will take 1000 INR to buy a muffler-microphone or better if you get a wireless microphone and a good camera.your knowledge is very good so try to get better recording environment, even you can hire a professional on very nominal cost.

[@gokhandemirkiran1134]

Very articulate.

[@siamecourage5842]

You saved me 👏

[@shomusbiologyofficial]

You're welcome

[@gangaraju2731]

sir plz eplain TED and TDD algorithms

[@bolesathvika3708]

Plss explain the cap analysis topic plsssdddd

[@fahadalam482]

what is RNA expression???

[@KinGamerBoy568]

Discuss how you would assess whether your target gene is being translated Can you please answer this Q🙏

[@dr.pratishthasharma6443]

please do a lecture on microbeads

[@mjustsarifizu]

what about differential gene expression analysis?

[@tonial5789]

I don't understand, so if you take cDNA at3 time gaps do you need to use three different chips since you are tagging all cdna red then you will now know what time point the resulting color in chip will come from.

[@pratyushabehera4561]

Sir..whats the need of isolating mRNA..why can't we take the dna of the sample cells directly..plzzz help

[@bolesathvika3708]

Please explain cap analysis with diagram plssssss