EdvoTech Tips: What is a DNA Ladder and how do you use it?

[@christopherlofgren9747]

Correct me if I am wrong, but I was looking through the Edvotek 301 lab and based on the EcoRI digestion sites, wouldn't a single insert in either direction result in the same fragment sizes of 3000 bp for the vector ad 1282 for the insert. Determining orientation of insert would not be possible with gel electrophoresis. In the video, at 1;57 if the blue region represents the insert, there is a digestion site for for EcoRI that is within the sequence of the insert and not at the end (I believe this contradicts the sites found in the Edvotek 301 manual.) Thanks for your help with this matter.

[@EdvotekInc]

Hi! Great catch. I can see why that was confusing. The complex plasmid map was show as an example of a plasmid map, but the actual restriction digest performed and analyzed was a simpler one that could be used to show directionality of an insert. As it says in the video description, the plasmid used in this exercise was not for commercial sale.

[@kalukaluigwe9752]

Thanks 🙏. Good

[@EdvotekInc]

Glad it was helpful!

[@MrPianoMatt12]

Dr daniel snowflake 😍😍😍😍😍😍

[@EdvotekInc]

Yes, that is her name!

[@shahonur9432]

b

[@EdvotekInc]

Hey, think you might not have finished your comment?

[@sofiasmiley]

Why is it called DNA Ladder? Is it just because it looks like a ladder? I know nothing about biology or science (and I don't know why I'm watching this video 🤣)

[@EdvotekInc]

Yes, that's it! Each band is a different size and they look like a ladder when they are all run in the same lane. I hope you enjoyed the video! science is fun, and now you know a little more. Definitely check out more of our videos!

[@sofiasmiley]

@@EdvotekInc Thank you for answering my question!

[@EdvotekInc]

@@sofiasmiley Always happy to help a new science enthusiast! You can check out our blog for more information! blog.edvotek.com/