Endospore Stain Procedure (Moeller and Schaeffer-Fulton Methods)

[@rosea3285]

I pay for a college course and your a better professor than what I currently have. I've been teaching myself and your videos have been so helpful! thank you!

[@gracejohane9063]

You explain stuff so well with the sweetest voice ever.thank you so much you've helped me a lot.

[@sciencenerd752]

I searched this topic so much and finally found this video. Thanks a lot... Only gram staining is much talked about but you made me help endospore staining ❤️

[@kuruptgt]

calm. concise. clear. I love all your lectures on biology! thank you

[@joleenaah]

love your videos! I got a micro lab midterm on monday and these helped sooo much ( :

[@RaviranjanKumar-nd3pj]

Nice video, i definitely hats of ur talent , the way u explains the topics really amazing ❤️

[@sharadsingh96]

Your video is very very best mam

[@idrisgamous4895]

Brilliant is she, I like her way in teaching

[@BiologyProfessor]

Deon Murray Some species of bacteria (but not all) go into an endospore state when conditions for growth become really unfavorable and, yes, high temperatures are one such condition.

[@raymoleon]

You're a wonderful teacher ! I'd like to know if heating the bacteria would put it in an endosporic state?

[@artisticjunction6903]

No... Endospores form only when bacteria are in very harsh conditions... Just like lack of nutrition..(This stage is a survival strategy) Heating just kill the bacteria.. Hope you understand...

[@prathapagiriharish2687]

Nice explanation than our college teacher explanation.

[@raymoleon]

Biology Professor Sorry I meant when it is heat fixed to the slide.. wouldn't that put all the bacteria in an endosporic state?

[@sogolghiasvand7205]

your videos are fairly useful and understandable , thank you so much

[@sallyrahma608]

Thank You

[@omaralrawi9855]

Time for ather stage?

[@thecatepillar]

You are wonderful. Thank you for the help.

[@solangeabbate7099]

I'm a little confused as to how this is different from the acid-fast stain. Doesn't that also use carbolfuscin and methylene blue?

[@BiologyProfessor]

Yes, the Moeller endospore stain uses the same stains as the acid-fast stain. There is a longer "steaming" step between dyes than in acid-fast, but they are pretty similar! en.wikipedia.org/wiki/Moeller_stain

[@solangeabbate7099]

Interesting.

[@gilong1311]

Hello, is it preferred to get the sample being placed on the broth be from a slant or a broth?

[@BiologyProfessor]

I don't think it matters.

[@catiedodge6661]

I love your videos, but I think this one should be using Malachite Green and Safranin to identify endospores. The one you are describing here is the Acid-Fast stain. Or am I wrong?

[@BiologyProfessor]

I go over that at 4:30. That’s the Schaefer-Fulton method.

[@lunarhealer6667]

Do bacteria decompose? Why are we able to keep slides for so long without them breaking down?

[@BiologyProfessor]

Yes, bacteria decompose. Microscope slides last a long time because the bacteria are “fixed” or “preserved” in some way.

[@장한해]

Hi I'm a high school student and I'm trying to do this experiment (endospore staing) and i got problems buying malachite green soultion. So the point is do u know where to buy it or how to make it? And one more thing do u know the difference between malachite green and malachite green oxalate??? Fast answers would really help me thanks !!!!!!

[@hfjones2663]

Very very helpful !!

[@michaelkebede552]

you are the best!! Thanks!!

[@anilkundu7207]

Mam please teach in hindi or simple words

[@sajibislam1733]

Still alive ❤️❤️❤️

[@mateba5647]

I thought the primary stain was MALAQUITE, and the SAFRANIN?

[@shivamsahil3660]

Would normal home detergent work?

[@BiologyProfessor]

I really don't know. I guess you can try it and see... Good luck!

[@djinn8660]

Awesome video. thanks

[@ewwxaca]

Thanks so much

[@Sassy-Vixxen]

Your videos are great and easy to understand. However, you have a mistake in this video. In the Schaeffer-Fulton method, you do not decolorize with acid alcohol. I would adjust your video to reflect this, as the way it is described sounds like both methods require the destain.

[@mohdislamansari5615]

Hello mam

[@agnumajumder9000]

u r lovely

[@adamescobar7554]

Came here to learn about staining.......now completely distracted by how beautiful you are. Not getting any studying done now :p