FIJI (ImageJ): Image Enhancement with CLAHE [Contrast Limited Adaptive Histogram Equalization]

[@polyfishcat7179]

I learnt from your video and made my first 3D animation of a z-stack imaging via 3Dscript. Also, all the music in your videos is so amazing!

[@johanna.m.dela-cruz]

Thank you for your support. It means a lot.

[@barrigaverde6957]

Hi I need to analyze the shape (circularity, roundness, esphericity etc) of particles in a few hundreds images, the usual filters and masks are not enough to reduce the pixelation so the Analyze Particle will return a value of 0.901 even for a perfect circle while using Morphological Segmentation from MorphoLibj, the values of circularity will be 1 or .999. The error is directly proportional to the particles size, the larger the particle, the greatest is the error. (The perimeter measumerent error is greater than the Area). Circularity = 4*PI(Area/Perimeter^2) But the Morphological Segmentation can be only done once image at the time. I tried to make a Macro, but it didn't work. I need to slighty smooth the particles boundary.

[@johanna.m.dela-cruz]

Hi there! I believe your question was aptly answered by Herbie500 (Reddit). I agree with him that a macro would be ideal.

[@barrigaverde6957]

​@@johanna.m.dela-cruzYes, but thanks anyway for your attention. His macro and explaination couldn't be more helpful.