FISH - Fluorescent In Situ Hybridization

[@henrikslab]

Which topics should be next? Please make suggestions here:

[@harikabharadwaj98]

DNA fingerprinting

[@nbijetadevi1719]

Microarray

[@philippphilipp9832]

Bacteriophage typing method

[@darshanhegde7717]

Karyotype versus FISH versus microarray versus targeted exome vs clinical exome

[@shukuraopeyemi6670]

Qq% 1%q@a1@100

[@gabrielanunes146]

English is my second language and the way you talk very slow was just amazing for me, because I'm studying about FISH in my university in Brazil and I'm so proud of me to studying in English. By the way I have a test today wish me luck!

[@mariociencia12]

This is the best video about FISH that I saw on KZbin!

[@siyabendunus6443]

Thank u. Nice german accent ;-)

[@thetrollpatrol8799]

Why the wink

[@niels1318]

@@thetrollpatrol8799 ;-)

[@cfuenza4106]

Extremely awesome and relevant to the preimplantational genetic testing environment. Well done, clearly explained!

[@Augetie]

This helped me so much thank you for explaining in such a calm voice and tempo

[@Augetie]

@Mixkopf 38 it helped me lmao

[@Vagolyk]

Never thought this is how fish are made.

[@telugufunnymoji5564]

How the deletion site is known . And what confirms that probe is bound like how it gives signal after binding to dna

[@utrlohan]

Excellent work, thank you very much for your time and effort.

[@耿显]

really clear and helpful....solved my problem about why we used small DNA fragments ,thanks a lot

[@englishspoken8168]

Cell fixation Adding probe Denaturation Hybridisations

[@doodoobearlove]

Thank you totally clear my confusion! and right to the points!

[@alaechda9056]

An exemple of how to design a probe for a specific gene will be nice

[@lilbits661]

Crazy German scientist over here. Thanks tho, it was very helpful!

[@nmz61]

Thanks, that was easy to follow and understand.

[@deniseoliver-stergiou7842]

Wonderful easy to understand video! Thanks Henrik!

[@RahulKumar-mm5vm]

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

[@anusharamdurga6943]

Really Thank u so much sir for this video ☺️ it helped me a lot to do my seminar ....God bless you 🙏

[@عزالدينالشيخسليمان]

Nice explanation, You made the concept easy and digestible, thanks

[@priyasaha183]

This video is very helpful to understand FISH easily

[@vlr003]

So helpful and clear, thanks!

[@MinhLe-nw2zz]

You try to give the video more brightness it will be great if you do

[@hollyashton7073]

Very helpful! have you done one of CISH?

[@rosette_renah]

This was soooo helpful,,thank you

[@deivibarci2093]

Thanks a lot :))) You just saved me 3 hours 🌟🌟 Keep up the good work !!

[@lexa5950]

This video was so helpful, thank you for your effort

[@Swati.ss567]

Very clear n concise... Thanks a lot really

[@meeromeer8812]

Thanks. I need to know how to figure out the used probes! For example: we have thousands of syndromes, do i have to make probes for all of them (especially if I have no idea about symptoms)?

[@ruanfamilytv9658]

2 Quick questions 1) how can the ligase seal the fluorescent nucleotides. They need to be bound first right? So my question is how do these labelled nucleotide bind? DNA polymerase will just randomly come along? 2) if i do the labelling before I amplify, how will the PCR be able to copy the fluorescent dntp? thank you

[@Pearlblack6387]

in my opinion, ligase has some recognition site that recognizes the specific sequences and then it binds and causes ligation. DNA polymerase also has some specific sites that recognize first and then do polymerization. is this correct?

[@anirudhmy4528]

Wow man very nicely done It was very helpful for me Thank a lot.

[@henrikslab]

anirudh my thank YOU for watching it and the feedback!

[@kui635]

Kurz und knackig, danke!

[@thaliahurtadozegarra8516]

thank you very much for this video!!!!,I understand all

[@franciafenovavy6608]

After denaturation, why will the target chromosome will hybridized with the probe instead of rebinding to its original strand?

[@dr.qaisarjan2440]

Greatly well explained

[@rosedeleema4217]

Warm greetings. The short video is very helpful . But how to identify the specific complementary site in a chromosome . Or how do we know to prepare the complementary probe , on what idea this can be produced? If possible kindly send the answers. Thank you. Dr. Rise de leema .

[@henrikslab]

Usually you know the sequence of the complementary site since it is a gene you can look up in genome databases! Best Henrik

[@trivapatel1915]

Helped a lot thank you so much☺️

[@meeromeer8812]

Thanks. Is it possible to tell which test do I have to do when I'm facing a syndrome?

[@henrikslab]

I would ask that a medical doctor!

[@santyadel6323]

That was very helpful, thank you

[@erkegirl]

This video was real good!

[@tanjiawen7467]

very detail and clear. thanks a lot

[@riamirto5002]

Thank you! Really helpful!

[@taongamvula3313]

Where is the DNA that the probe originates come from?

[@henrikslab]

The DNA probes for FISH can be ordered by companies which will synthesize them. Is that what you mean?

[@mohammedal-hammadi5085]

It's so helpful and clear video, thank you so much really

[@dortartakovsky7123]

How you can amplify the flourocent probe by PCR? it wouldn't increase the amount of the probe which is tagged

[@sonalimali8160]

Very nice for understanding thank you

[@mithratpmithra]

Is this a technique in genome mapping?...

[@henrikslab]

One of many, yes

[@Bror円]

This is great!

[@zglrd8938]

Does interphasic fish allow us to detect both anuploidie and microdeletions or just anuploidie?

[@medicaleducation8978]

some time it gives false negative results because some time probe are not properly designed and sometime there is change in annealing temperature ? Am I right sir or any other reason of false negative result ?

[@ameliac504]

THANKS! NEEDED FOR A PAPER

[@kubranuraydn5036]

very good video.

[@wuming_yd]

do you heat the cells/tissue up to 95 C? will the cells/tissue be destroied by such a high temperature?

[@xaviasturm7248]

Gibt es auch ein Video auf deutsch ?

[@lexc8714]

So FISH can help in karotyping too?

[@henrikslab]

Yes

[@siniorashani]

Thanks very much ❤

[@anisa7c]

how to make sure that the probe is fully hybridized not the complimentary DNA ? thanks :)

[@henrikslab]

First of all: Good question! My answer is based on this paper: www.sciencedirect.com/science/article/pii/S259000721830008X If you make sure that the concentration of the probe is way higher than the concentration of the target dna (e.g. 10000 probes per dna strand) it should be way more likely that one of these probes bind after denaturation compared with the single sister strand of the dna Hope you could understand my explanation!

[@sangeethap5197]

Useful sir 👏

[@ravioli6301]

Is it correct that a ARRAY CGH is kinda a reversed FISH? U should def do a video about micro array especially the array CGH as it is also used for chromosomal DNA deficts :)

[@zaysanatomy]

So cool! Thank you!

[@Zdnew]

👏👏

[@Meowmeows24]

What is the principal and the concept of the FISH technique ?

[@henrikslab]

It is to detect specific sequences on the patients chromosome (which are altered in disease). With FISH we make this sequence of interest visable using a "fluorescent probe".. this labels the sequence of interest

[@Meowmeows24]

@@henrikslab u help me , thank you SM😣🤍🤍🤍

[@sapthakathilakarathne242]

thank you !!

[@zoew7872]

Can FISH detect mosaicism in an adult?

[@englishspoken8168]

Fixation of cell et formaldehyde

[@priyankaburange5832]

Thank youu !!

[@daenalund1613]

Very helpful thank you so much!

[@idrisbagaruwagwarzo4363]

I really do appreciate you Dr Igudia youtube,your commitment towards saving human lives is steadfast ,all the knowledge I got from you and,the help with the herbal meds, I was recently tested negative of Hepatitis B infection,I’m so happy thanks doc

[@arnotrihalder1237]

very helpful

[@jaafar2562]

Did anybody learn this in grade 12? because in lebanon we do .

[@sylvia_forest]

Yes I do. I'm learning this as a new chapter.

[@jaafar2562]

@@sylvia_forest ah ok thx

[@manishjadonrajput3095]

Thank sir explain mutations.types like this.

[@buddha7390]

Thank u sir

[@bushrazaman9882]

Thank you

[@ashirt2100]

Thank you:)

[@englishspoken8168]

Fluorescent in situ hybridization

[@leilaaryan1575]

👍

[@sadahauch910]

Is Nick translation And FISH hybridization is same

[@henrikslab]

That is a good question, but here is the answer: So nick translation is just the process of getting your labelled probe. With this probe you can do whole process of hybridization then (FISHybridization).

[@Chelssums]

THANK YOU!

[@tinomaster12]

Thankyou!

[@حيدرعباسعلى-و5ف]

THANKS FOR YOU

[@fabianperson]

Nice accent man!

[@herbalvision2134]

it's really interesting, please stay connected :-)

[@anamoirangthem3817]

Sir I want videos for chromosome walking

[@englishspoken8168]

Add probe

[@whitetomato]

I don't understand... You never explained about the fish. Clickbait

[@englishspoken8168]

Denaturation

[@chemistrytutorvideos3801]

you sound soo german :)

[@sagarsanse8701]

Please hindi me bolo

[@earthworm-filledstomachbyc4254]

Seek Christ Jesus YHVH.

[@leonneumann2872]

sprich doch deutsch henrik

[@sostyles14]

thank you

[@Federico-mj3si]

Thanks!

[@tlechem9]

THANK YOU 👍👍👍