I find a mistake in the statement made at 3:19 min "If we get arc-shaped band..." because if the arc-shaped band is obtained it simply means that the two antigens have a set of identical epitope groups with respect to the antibodies used. Although the two antigens can differ in other epitope groups but the antiserum used may lack antibodies to those groups. So this arc pattern does not mean that the antigens are identical and doing so is wrong.
@FrankLectures6 жыл бұрын
+Shubham B. Deshmukh thank you for pointing out the mistake. Yes, the correct statement should be... "If we get arc-shaped band, this means the given antigens have identical epitopes or antigenic determinants".
@shubhamb.deshmukh79206 жыл бұрын
Hey! Thanks for appreciating. Well, your video made me think better :)
@FrankLectures6 жыл бұрын
thanks and hope for more feedback like this from you in future.
@pamelamkhize2855 жыл бұрын
@@FrankLectures Hi there! I am a Lecturer at the University of KwaZulu-Natal. I have used some of your videos for educational purposes. Thank you for your excellent videos.
@FrankLectures5 жыл бұрын
@@pamelamkhize285 Hi. Glad to know that these videos are helpful to you. Thanks you for appreciating my efforts. It really means a lot. 🙂
@littleluck35275 ай бұрын
I didn't find your videos when I was taking Immunology class, but I find them when I'm preparing my certification exam. Thank you so much for those short, clear, direct videos. I like the logic and the steps that how you present these to students.
@dikshitkapil46864 жыл бұрын
This was really the first time i could understand this procedure. Thank you for making this topic so easy.
@arghayeaye4591 Жыл бұрын
such a clear and time efficient explanation
@t-alimichael3363 Жыл бұрын
OMGosh all these videos and finally a robot explained it best that makes sense.
@husseinqasim41723 жыл бұрын
I'm dr ruqaya from Iraq , thank you so much for excellent video 📹
@shruti574 Жыл бұрын
Thank you Ma'am for your videos. They are soo good 💫. Stay healthy and happy 💫💫
@icshahid3 жыл бұрын
To be honest before watching the video I had no idea of what DID was despite doing it in lab . But after watching o felt that I know everything about it. Bravo creater !
@peaa6663 жыл бұрын
This channel is so freaking good. Illustrations are so good to understand. I wish the videos were in human voice. Keep up good work👍👍❤❤❤❤
@knoshamaryam2666 жыл бұрын
very simply put and illustrated to understand , great work , thank you :)
@FrankLectures6 жыл бұрын
Glad to know this. Thank you for your feedback.
@cathg.9563 жыл бұрын
im so happy youtube exist nowadays with all this illustrations and graphics lol on my college days 12 yrs ago it is difficult to understand by just reading the book
@wafaqa42365 жыл бұрын
Very clear explanation for non-English speakers
@FrankLectures5 жыл бұрын
Thank you for your valuable feedback.
@sherilramos91677 жыл бұрын
Thanks for sharing it! It was really helpful to look at this perspective
@FrankLectures7 жыл бұрын
You are welcome :)
@suhagroy50884 жыл бұрын
THAT IS A VERY NICE VIDEO WHICH HELP ME TO UNDERSTAND THE TOPICS MORE EASYLY.JUST CARRY ON
@sanjanagupta8372 Жыл бұрын
Great info ... Thank you
@inna76104 жыл бұрын
Thank you so much to save my quarantine semester :,v
@albertamensah90484 жыл бұрын
Thank u very much.....Really helpful
@MohammadHQadir6 жыл бұрын
Thank you so much for such a useful video 🌹💜
@FrankLectures6 жыл бұрын
Thank you for your feedback.
@mattamounika29846 жыл бұрын
Fantastic explanation
@FrankLectures6 жыл бұрын
Thank you.
@notanurse23735 жыл бұрын
For the love of god, find someone to read your script who is human and can pronounce such complex words as "agar."
@vinamratasharma76982 жыл бұрын
😂
@wanderer372 жыл бұрын
For the love of god , find some gratitude in your heart 😊
@jaydeepdumadiya24643 жыл бұрын
Jordar video hto.... Love from gujarat , INDIA🔥🔥🔥
@aaliabhatt58456 жыл бұрын
Thanks loads... For the video... Helped me alot... 😊
@FrankLectures6 жыл бұрын
Thank you for your feedback
@manognakuchibhotha70183 жыл бұрын
very informative video and clear explanation... thank you
@mohitsinha19836 жыл бұрын
Thank you so much
@FrankLectures6 жыл бұрын
You're Welcome
@chaimamouas218 Жыл бұрын
Great work thnk u 🇩🇿🇩🇿
@superbscientist7434 жыл бұрын
For non-identical antigens (a and b), do we need to have information about their epitope... ? So that we can decide that which two antibodies to be used (as you said antibody A and antibody B to be put together).
@amaniewallace70215 жыл бұрын
This was helpful. Thanks!!!
@FrankLectures5 жыл бұрын
Thanks
@rohinayameen69614 жыл бұрын
Thankyou so much, very easily explained ♥️
@nhanguyenlt72256 жыл бұрын
thank you, very comprehensive
@FrankLectures6 жыл бұрын
Thank you for your feedback.
@vikasraghuvanshi85516 жыл бұрын
Nice way of explanation...
@FrankLectures6 жыл бұрын
Thanks
@vikasraghuvanshi85516 жыл бұрын
Frank Lectures your welcome.
@drrrajkumar262 Жыл бұрын
Wonderful explained ❤
@nemanjatepac2 жыл бұрын
What is unknown Ag or Ab? What we want to find?
@israchan4474 Жыл бұрын
Thank you 🌸
@mustajabalam37944 жыл бұрын
Great help plz make videos on QC of RID , Elisa ,IF , SPE ,and flow
@yahyakhaled75974 жыл бұрын
One confused point for me , is ab is specific to both non identical ag at same time !! And if that is true so how can we depend on precipitation test for diagnosis of ag or it's used only for determination of concentration and identification !!
@superbscientist7434 жыл бұрын
Me too have same query....
@vadoelz4 жыл бұрын
Hello, thank you for this lecture, but can you explain or list the equipment that need to use and also the reagents that will need to use? Thank you.
@wonderful38412 жыл бұрын
Tq so much. It helps me a lot.
@somashishsaha2 жыл бұрын
Excellent
@immortalspirit97788 ай бұрын
Thank u well explained
@aldanarosenbrock65375 жыл бұрын
Me encantó! Muchas gracias !!!
@FrankLectures5 жыл бұрын
Thanks
@jeremymoore1454 ай бұрын
thank you!!!
@bhajan42903 жыл бұрын
Awesome!
@josef91415 жыл бұрын
thanks alot this was so helpful
@FrankLectures5 жыл бұрын
Thanks
@user-vg2nn1bu7b6 жыл бұрын
thank you soo much you helped me many times
@FrankLectures6 жыл бұрын
Glad to know this. Thank you for your feedback.
@sainirajni7835 жыл бұрын
Thnkuu so much for making video on this topic
@FrankLectures5 жыл бұрын
Thanks
@docshaheen67416 жыл бұрын
Awesome video again,
@FrankLectures6 жыл бұрын
Thanks
@magdahassan8052 Жыл бұрын
Thanks❤
@mansijogi4603 жыл бұрын
Thank you 😊
@bornsupremacy84 жыл бұрын
Thank you
@maramnawaiseh80516 жыл бұрын
Awesome.. go on♥
@FrankLectures6 жыл бұрын
+Maram Nawaiseh Thank you.
@dusk01352 жыл бұрын
Perfect!
@HaneenFaizalHaneenFaizal9 ай бұрын
How can we use 2 antibodies in 2nd case, since we dont know whether the antigens are identical or not🙄 we are testing that no?
@fatimao3li9553 жыл бұрын
Mercie
@bluegirl80586 жыл бұрын
I'm MLT student, thank you
@FrankLectures6 жыл бұрын
+Blue Girl you're welcome.
@viktorpirozi25466 жыл бұрын
Many thanks for this video, it was very helpful to me.
@FrankLectures6 жыл бұрын
+Viktor Pirozi thank you for your feedback.
@dariocosemans91516 жыл бұрын
Hi, Sorry for my Flemglish (English with some Flemish in it) :) Here I go :P Can someone help me with this question? What makes the diffusion possible? The difference in concentration of course, but does the form, mass or friction forces take part as well or are these factor negligible? For example: When doing a non identity, you have three gaps. In the first one you put epitope 1 , the second one you put epitope 2 and epitope 4 (2 different antigens). In the third gap you put, three different antibodies, respectively Ab1 Ab2 and Ab4. When getting a pattern you will obtain 3 lines that will cross. You have 2 lines that are closer to each other (one line is from Ag2-Ab2 and the other one is from Ag4-Ab4. But the question is which line is from which antigen-antibody interaction? In class the professor told us that the line that is most far away of the antigengap (epitope 2 and epitope 4 ) is the one of the antigen with the highest concentratie. But is this it, or can there be something to add at the profs explanations ?
@FrankLectures6 жыл бұрын
What I have studied is that the rate of diffusion is affected by other factors also such as size of the molecules, the temperature, gel viscosity, amount of hydration. And regarding your second question, it is difficult to answer. Need to study more for this :)
@wafaqa42365 жыл бұрын
Thank u 👍 🌷
@FrankLectures5 жыл бұрын
You're welcome
@Girlinterrupted9554 жыл бұрын
Thanks 🙏🏻
@rupalipawar27874 жыл бұрын
Good!!!
@rupalipawar27874 жыл бұрын
Good
@simrankaur24713 жыл бұрын
Ty
@mustajabalam37946 жыл бұрын
Really helpful ...thanks
@FrankLectures6 жыл бұрын
Thank you for your feedback
@evaortega56315 жыл бұрын
Hi, this this technique is not cuantitative??thanks
@FrankLectures5 жыл бұрын
It is a quantitative technique.
@stz12386 жыл бұрын
thanku😊😊
@FrankLectures6 жыл бұрын
You're Welcome.
@user-bp4oi9hb3c2 жыл бұрын
Thnx
@rouaalharby29872 жыл бұрын
Tnx✔️
@ibrahimfaisal53154 жыл бұрын
How can i get the antigen in the test we used two antigen and one antibody. one of these antigens is control with the kit ok,, but the secined how i can get it?
@rakshitapawar12426 жыл бұрын
Apt explanation!
@FrankLectures6 жыл бұрын
+Rakshita Pawar thank you for your feedback.
@k.rajkumar86504 жыл бұрын
Please tell us about Rocket immunoelectrophoresis
@alyaae.h35336 жыл бұрын
شكرا جزيلا
@FrankLectures6 жыл бұрын
بكل سرور
@thedolphinbro66684 жыл бұрын
ty!!!
@sarasettou67173 жыл бұрын
Thank yoouuu❤
@imenemezioud24605 жыл бұрын
Thanks
@FrankLectures5 жыл бұрын
You're Welcome
@Dhanapraveen8514 жыл бұрын
Super
@neemabisht90916 жыл бұрын
I didn't understand the non identical immunodiffusion reason why they make cross sign
@FrankLectures6 жыл бұрын
There are two different antibodies in the antibody well. e.g. antibody A and antibody B. Each of the two antigen wells contain different antigen. e.g. antigen a and antigen b. When immunodiffusion will occur. Antibody A will form immune complex with antigen a for which it is specific. Similarly, Antibody B will form immune complex with antigen b. They will form precipitation lines at their respective zone of equivalence. One precipitation line will be of immune complexes made up of antigen a and antibody A. And second precipitation line will be of immune complexes made up of antigen b and antibody B. So, these precipitation line formed are actually two different precipitation lines which appear to cross each other. To understand better imagine these two precipitation line having two different colours.
@auxjosemexixi10894 жыл бұрын
Tnq
@habibinoor73496 жыл бұрын
Thankoo so much
@FrankLectures6 жыл бұрын
you're welcome.
@habibinoor73496 жыл бұрын
😊😊😊😊
@rohanerohane91424 жыл бұрын
Traduction français avec la description svp
@norahhakami36473 жыл бұрын
ياليت الصوت يتغير
@yusra94283 жыл бұрын
Very well explained but the voice is so annoying! Anyway thank u🤙🏽
@mubeenajasif87754 жыл бұрын
Mubeena
@carlavargaszavaleta94195 жыл бұрын
Excellent
@FrankLectures5 жыл бұрын
Thanks
@yahyakhaled75974 жыл бұрын
One confused point for me , is ab is specific to both non identical ag at same time !! And if that is true so how can we depend on precipitation test for diagnosis of ag or it's used only for determination of concentration and identification !!