Рет қаралды 63,910
RIEP known as electro immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen in an unknown sample. Various concentrations of antigens are loaded. On electrophoresis, the antigen begins to migrate towards anode. Antigen and antibody interacts, when it reaches the equivalence point, Ag-Ab complex precipitates. The precipitation line is seen in the form of a rocket.