Radial Immunodiffusion (Mancini Technique) (FL-Immuno/57)

[@RoshanSingh-rv3vx]

worthful video and explanation...very easy to get idea about Immunodiffusion but in incubation require 48-72 hr which depends on the mol.wt of Ag, for IgG quantitation require 48 hrs and IgM requires 72 hrs of incubation and concentration of Ag is related to the square of the diameter of the precipitin ring.

[@FrankLectures]

Thank you for your feedback and the additional information.

[@hfhf94]

Thank you,well explained ,do more videos🤗🤗

[@rohinayameen6961]

So easily explained ♥️

[@Ben_Palmer.-ON-TE.LEGRAM.]

Wonderful explanation. But need the video to always watch it for more understanding and retaining. How do o download it

[@يارب-ف3س1ش]

Thank you very much جزاكِ الله خيراً♥️🤲

[@nirmalaselladurai1433]

This video is very helpful to me...

[@Benitahbherosecondchannel]

Thank you for this simple explanation

[@viktorpirozi2546]

very useful and helpful video ,Thank you

[@FrankLectures]

+Viktor Pirozi thank you for your feedback

[@organicspectroscopy7242]

Thank you very much for this video! But I have had a question for a long time. Usually, the standard curve is done on a Semi-log graph.. with the concentration being plotted on the logarithmic X-Axis. But I wish to know why it is done so, instead of using a normal graph. Does anybody know why?

[@FrankLectures]

You're welcome.

[@nishapaudel5572]

You can draw on normal graph as well but they need really reallybig graph sheet ..there diffrence is in log ratio so its convinient to use log sheet .

[@bernardberne353]

Corrected message: There are two methods for quantitating antigens in radial immunodiffusion. In one method (kinetic method or timed-diffusion method), the diameters of the precipitin rings are measured while all rings are expanding (usually within 24 hours, depending upon the antigen and the temperature). In that method, a graph plotting the diameters of the rings on a linear X-axis and the antigen concentrations on a logarithmic Y-axis produces a straight line. In the other method (equivalence method or end point method), the diameters of the precipitin rings are measured after all rings have reached their end points and have stopped expanding. In that method, a graph plotting antigen concentrations on a. linear X-axis and the squares of the diameters on a linear Y-axis produces a straight line. When some precipitin rings are still expanding, but other rings have reached their end points, both types of graphs will produce lines that are partially straight and partially curved. See the Wikipedia article entitled "Radial immunodiffusion" at @t for more information about these measurement methods.

[@bernardberne353]

Second corrected message: There are two methods for quantitating antigens in radial immunodiffusion. In one method (kinetic method or timed-diffusion method), the diameters of the precipitin rings are measured while all rings are expanding (usually within 24 hours, depending upon the antigen and the temperature). In that method, a graph plotting the diameters of the rings on a linear X-axis and the antigen concentrations on a logarithmic Y-axis produces a straight line. In the other method (equivalence method or end point method), the diameters of the precipitin rings are measured after all rings have reached their end points and have stopped expanding. In that method, a graph plotting antigen concentrations on a linear X-axis and the squares of the diameters on a linear Y-axis produces a straight line. When some precipitin rings are still expanding, but other rings have reached their end points, both types of graphs will produce lines that are partially straight and partially curved. See the Wikipedia article entitled "Radial immunodiffusion" at dial immunodiffusion" at @t for more information about these measurement methods.

[@organicspectroscopy7242]

@@bernardberne353 Thank you very much for your informative and detailed reply! In the last two years, I did finally get the answer, but nonetheless your reply is more detailed and thus still much appreciated. Thank you very much! I am sure others who have the same question will appreciate your reply too.

[@aniokechukwudi2472]

Thank you so much for this ☺️

[@hephsibajyothirudrapogu9616]

Nice video.. Easy to understand.. Thank you

[@FrankLectures]

Thanks

[@bioworld8132]

Ur video are just awesome thanku

[@loannguyen7739]

thanks you so much, this video is very helpful for me

[@docshaheen6741]

awesome explanation

[@FrankLectures]

Thank you

[@nikitadorzhin7969]

Character In the video It's great, I like it a lot $$

[@saoodakashmirii2569]

Very helpful video .. ❤️

[@vladimirkocic9952]

Thank you so muh!

[@FrankLectures]

You're Welcome.

[@shoshohamad9324]

Thank you very much for this offer

[@FrankLectures]

thanks for your feedback

[@wafaqa4236]

Brilliant. . As u used to be🌹🌹🌹

[@aartiyadav4367]

Best one👌🙌

[@FrankLectures]

Thanks

[@localasian]

Thank you. What are the books you're using in immunology

[@SoniaDiversion]

Thank you!

[@FrankLectures]

You're welcome

[@anniezhu3961]

Anda boleh memilih untuk salah satu daripada hadiah di atas

[@HarpreetKaur-cj6if]

it is also known as single diffusion in two dimensions?????

[@ياسررياضحازمفتحي]

What is the amount of Abs in the gel & and how the Ag reach the optimum concentration with Ab in the gel ?? 🤔🥰

[@shrutikaumredkar2593]

Thanks:)

[@AJeetSingh-gj7hs]

what happen with antigen of unknown conc.

[@prabjotkaur5244]

thnkuuu so much

[@FrankLectures]

You're welcome.

[@thedolphinbro6668]

ty!

[@rawyhjamal5215]

Thanks

[@thewaytoallah4159]

good

[@cruzealone4252]

Tq

[@japoniasirinita5070]

😊😊😊

[@مؤملعلي-ش6ر]

Not video in his head 60 luky

[@user-sn7qv3gf6l]

هاي شون دبرتها هههههههههه

[@anishagulgule04]

Best ... thankyou