Functional Annotation of Sequences using Blast2GO (ver.4.1)

togotv.dbcls.jp...
Blast2GO is a user-friendly bioinformatics platform. This tool can run high-throughput functional annotation of novel gene/protein set.
This video introduces fundamental procedures of Blast2GO (ver.4.1) using sample sequences. #togotv#DBCLS#bioinformatics #togotv#DBCLS#bioinformatics

[@lizcumpa-velasquez4381]

Thanks, it's a good Video! I have a question. How do you know the time that Blast2Go needs for the Blast´s aligment?. I mean, a week is a very long time, if you are not using the cloud ... How Do you figure out with the pc? Does it never turn off?

[@togotv]

Thank you so much for watching. This is the actual time that was measured when this video was created. Using Blast2go, it doesn't seem to be possible to get estimated time in the software when submitting a query to NCBI BLAST. (probably as well as running it on the NCBI BLAST website). In our example, the fact that NR is the target database has also affected the increase in execution time. Selecting the best size database for your BLAST search may help to shorten the execution time.

[@lizcumpa-velasquez4381]

@@togotv Thanks for the answer! But, what about the computing time? Does the computer turn off while the program was running?

[@togotv]

@@lizcumpa-velasquez4381 We missed your other question. In the example in the video, if you shut down the computer while it's running, the calculation results will be lost. If you need a long calculation time, it is recommended to use local Blast. Blast2Go seems to have that option available for a fee. Blast2GO Command Line Tools www.biobam.com/blast2go-command-line-tools/

[@lizcumpa-velasquez4381]

I see, I am running a Blast search just now (3 hours ago) for 700 genes . Well, thanks for the quick answers!

[@togotv]

@@lizcumpa-velasquez4381 We're sorry to say that text-only information doesn't tell us much about your situation. Can you contact the provider of BLAST2GO (which seems to be one of the functions of the OmicsBox)? www.biobam.com/quote/