How to Prepare a batch of Petri Plates from Dehydrated Culture Media

[@Aakif862]

Love from India ❤

[@anniesorkin]

This is a very good tutorial, thank you!

[@HardyDx]

Glad it was helpful!

[@MargretWanjiru-yo1zc]

❤ easy to understand good work

[@kaelthuzad4640]

thank you for the video , i usually never autoclave the media , specially not the selective. i test a small quantity for contamination and if it is okay i do not autoclave the , i just heat it for 10 muntes after the agar has totally melted and it does the job.

[@HardyDx]

For sterility assurance, we do recommend autoclaving the media. If you have any questions or want to dive deeper into the "why" of it, feel free to reach out to our Technical Services team at [e-mail], they're an amazing team who'd be happy to explain more!

[@ceciliaovansa2738]

It was nice watching

[@HardyDx]

Thanks!

[@kimondoDen]

Very insightful

[@HardyDx]

Glad it was helpful!

[@BAD5RO]

Informative

[@NiyibugingoValentin-dk7sk]

Thank your brother is here with me and I will get it more information

[@khushiak-4782]

The vedio is too awesome

[@JimohRahama-ur6kz]

Thank for the video, I really benefit from it

[@HardyDx]

You're welcome Jimoh Rahama! I'm glad you found it helpful

[@ZainabLambe-dj5hx]

Thank you for the video

[@salahaldinmohamed6959]

Thanks from Libay

[@HardyDx]

You are very welcome!

[@md.mehedihasansiam4489]

Make a video of spread plate & pour plate method.

[@user-hm2rl9ef5w]

Why did you put the hose inside the autoclave into Erlen?

[@HardyDx]

Thank you for your question. The hose is actually a temperature probe. As the video explains the probe can be placed directly into the media (which is in the Erlenmeyer flask aka “Erlen”). As the directions state: “Place the flask of boiling media into an autoclave to sterilize. If your autoclave is equipped with a temperature probe, the temperature probe can be placed directly into the media and the cycle parameters will be based on the readings of the probe.”

[@user-mj4co3ll6t]

4:34 Is it okey to add the supplement outside of the clean bench?

[@mirhina8727]

Thnks for such useful video

[@HardyDx]

I am glad it was helpful.

[@miaab7143]

Is it ok to autoclave for 1 hour and a half??

[@user-ch4wv4nn3c]

Очень интересная плита для приготовления сред

[@miltonkunda8912]

Why can't I work in one of your labs

[@Vijay-ol5se]

Should we add additional amount of agar agar for solidification?

[@redvelvet3479]

I made cetrimide agar and there are moisture on media but idk why

[@coffee_sloth]

Why does boiling for too long damage the media, but autoclaving at 120 degrees doesn't?

[@selenabulanart]

You can boil it longer than one minute, we do it all the time with no issues. However bubble formation should be avoided

[@LandonStrauss-hc1sc]

Sounds like John Lithgow.

[@roshanjannath6954]

How to pop up bubbles if don't have flame.....

[@blueraine13]

try using a pipette :)

[@HardyDx]

You can also try misting the inside of your empty flask with 75% ethanol, see if this helps!

[@ChrishaMacreeba]

Sterile Loop may help you out

[@kaelthuzad4640]

quicjly blow torch it , i use it in my lab

[@majumundurchanel2908]

gak ngecek ph mas?

[@HardyDx]

Good eye! We do actually check the pH of all our media for specific pH ranges. That video is in the works, and is coming soon. Stay tuned!

[@raomasoodahmad1221]

❤❤❤🎉

[@user-ju8jk1kl5w]

You are make a only false media make a real culture media

[@AmanExplorerBoy]

What do you mean by that